Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

Code CSB-E08261r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Alternative Names
Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
Abbreviation
TFPI
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 ng/mL-500 ng/mL
Sensitivity
1.95 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %91
Range %85-96
1:2Average %89
Range %84-95
1:4Average %93
Range %89-97
1:8Average %90
Range %85-94
Recovery
The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-107
EDTA plasma (n=4)9288-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1OD2AverageCorrected
5002.596 2.474 2.535 2.424
2501.978 1.902 1.940 1.829
1251.172 1.096 1.134 1.023
62.50.687 0.702 0.695 0.584
31.20.401 0.424 0.413 0.302
15.60.282 0.291 0.287 0.176
7.80.182 0.175 0.179 0.068
00.113 0.108 0.111
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat TFPI ELISA Kit was designed for the quantitative measurement of Rat TFPI protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

Citations

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
Gene References into Functions
  1. TFPI expression is induced in cardiomyocytes, but not in endothelial cells, by IL-1beta. PMID: 11816723
  2. Rapid atrial pacing acutely downregulated the gene expression of thrombomodulin and TFPI in rat atrial endocardium, thereby inducing local coagulation imbalance on the internal surface of the atrial cavity PMID: 14610015
  3. TFPI levels decreased significantly at 24 hours in burn, septic injury, and burn+septic injury groups, followed by incomplete rebound recovery at 72 hours PMID: 15497025
  4. This is the first report showing that direct inhibition of TF-mediated coagulation activation abrogates bleomycin-induced pulmonary fibrosis. PMID: 16338226
  5. analysis of tissue factor changes and the effects of tissue factor pathway inhibitor on transient focal cerebral ischemia in rats PMID: 18067952
  6. The results demonstrated that overexpression of TFPI gene might induce VSMC apoptosis in vitro through the mitochondrial pathway; meanwhile, IAP-1 expression is decreased. PMID: 18368493

Show More

Hide All

Subcellular Location
Secreted.
Tissue Specificity
Most abundant in heart, lung, kidney, and aortic endothelial cells.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1