Rat apolipoprotein A1 (Apo-A1) ELISA Kit

Instructions
Code CSB-E08105r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-I
Alternative Names Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
Abbreviation APOA1
Uniprot No. P04639
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %94
Range %87-99
1:400Average %92
Range %84-96
1:800Average %84
Range %85-106
1:1600Average %97
Range %91-105
Recovery
The recovery of rat Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-101
EDTA plasma (n=4)9187-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mOD1OD2AverageCorrected
102.687 2.879 2.783 2.636
52.481 2.490 2.486 2.339
2.51.927 1.939 1.933 1.786
1.251.353 1.387 1.370 1.223
0.6250.895 0.874 0.885 0.738
0.3120.592 0.571 0.582 0.435
0.1560.372 0.399 0.386 0.239
00.148 0.146 0.147  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:


My samples are hemolyzed....could it be any problem with this samples when using the kits?

A:
Thanks for your inquiry. Serious hemolysis is not suitable for testing. You need to re-collect the samples.
If it is slightly hemolyzed, the impact is small and the samples can be used.
The sensitivity of this kit is relatively good, but we still advice you to use dual-wavelength detection to eliminate the impact.

Target Data

Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Gene References into Functions
  1. both the route of administration and the formulation of apoA-I peptide significantly affect its pharmacokinetics and pharmacodynamics. PMID: 27881716
  2. ABCA1 is diminished and ABCG1 is increased by N-acetylcysteine, which counteracts the reduction in apo A-I-mediated cholesterol efflux PMID: 25305669
  3. Apolipoprotein A-I induces tubulin phosphorylation in association with cholesterol release in fetal rat astrocytes. PMID: 24814947
  4. ApoA-I may be a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
  5. It maintains cholesterol homeostasis. PMID: 24117066
  6. on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels PMID: 22253414
  7. translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
  8. ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation PMID: 14993246
  9. The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
  10. Data show that a complex of apolipoprotein A-I and pregnenolone modulate the rate of protein biosynthesis in liver cells. PMID: 18457017
  11. ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation. PMID: 18535924

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Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons.
Database Links

KEGG: rno:25081

STRING: 10116.ENSRNOP00000065206

UniGene: Rn.10308

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