Rat heart fatty acid binding protein(h-FABP)ELISA Kit

Instructions
Code CSB-E16184r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
Alternative Names Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit
Abbreviation FABP3
Uniprot No. P07483
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %85
Range %84-92
1:2Average %96
Range %91-102
1:4Average %84
Range %81-88
1:8Average %94
Range %90-98
Recovery
The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-95
EDTA plasma (n=4)10395-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.095 2.086 2.091 1.967
5001.550 1.648 1.599 1.475
2501.121 1.156 1.139 1.015
1250.728 0.751 0.740 0.616
62.50.417 0.403 0.410 0.286
31.20.241 0.237 0.239 0.115
15.60.211 0.214 0.213 0.089
00.119 0.129 0.124  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Gene References into Functions
  1. Significant down-regulation of heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder. PMID: 21155221
  2. H-FABP might have potential to be an early and quantitative parameter of clinical severity and prognosis in CO poisoning. PMID: 20375122
  3. A complex interrelationship between glucose and fatty acids in the control of FABP gene expression; FABP3 and 5 may play a role in insulin secretion. PMID: 21099311
  4. rat myocardial FABP gene maps to chromosome 5q36 and was detected in a preparation of total RNA isolated from adult rat heart PMID: 10561574
Subcellular Location Cytoplasm
Protein Families Calycin superfamily, Fatty-acid binding protein (FABP) family
Tissue Specificity Heart, but also skeletal muscle, kidney, brain and mammary gland.
Database Links

KEGG: rno:79131

STRING: 10116.ENSRNOP00000017325

UniGene: Rn.32566

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