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| Code | CSB-E16184r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 81-88 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 103 | 95-108 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat heart fatty acid-binding protein (h-FABP) ELISA Kit is used to quantitatively measure rat h-FABP levels in serum, plasma, cell culture supernates, or tissue homogenates. It performs well in important characteristics, including sensitivity and specificity. This assay is based on the sandwich ELISA mechanism and enzyme-substrate chromogenic reaction. The solution color develops proportionally to the amount of h-FABP in the sample. And the intensity of the color can be measured at 450 nm via a microplate reader.
h-FABP, also called FABP3, is ubiquitously expressed in the heart, skeletal muscle, and other tissues. It participates in maintaining an energy supply to the heart and other body parts, as well as in regulating the intramuscular fat content and improving insulin sensitivity. FABP3 binds to free long-chain fatty acids (LCFAs) and transports them for cell metabolism, thus protecting against lipid toxicity. Studies have demonstrated that deficiencies in FABP3 exacerbate metabolic derangement in cardiac Hypertrophy and heart failure via the PPARα pathway.
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