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Rat soluble E-selectin,sE-selectin ELISA Kit

Citations (9) Protocol MSDS
Code CSB-E07996r
Size 96T,5×96T,10×96T
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Product Details

Target Name
selectin E
Alternative Names
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit
Abbreviation
SELE
Uniprot No.
P98105
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
78 pg/mL-5000 pg/mL
Sensitivity
19.5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:5Average %92
Range %88-108
1:10Average %94
Range %91-98
1:20Average %96
Range %89-100
1:40Average %84
Range %80-88
Recovery
The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9993-105
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
50002.746 2.645 2.696 2.542
25001.976 1.875 1.926 1.772
12501.228 1.204 1.216 1.062
6250.782 0.761 0.772 0.618
3120.522 0.511 0.517 0.363
1560.363 0.352 0.358 0.204
780.290 0.285 0.288 0.134
00.154 0.153 0.154 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Rat soluble E-selectin (sE-selectin) ELISA Kit is a powerful tool for immunology researchers to measure the levels of sE-selectin in a variety of sample types from Rattus norvegicus (Rat).

This ELISA kit has a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL, providing accurate and reliable results for even low levels of E-selectin. The assay time is 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.

The sE-selectin ELISA Kit utilizes a sandwich assay principle, where the E-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.

This ELISA kit is suitable for a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates, making it a versatile solution for researchers in the field of immunology. It has been cited in over 9 publications, highlighting its reliability and utility in research.

Citations

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Target Background

Function
(From Uniprot)
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Gene References into Functions
  1. The e-selectin/biliverdin reductase pathway participates in the inhibitory process of hypoxia in pulmonary artery endothelial cell apoptosis which is mediated by the mitochondrial-dependent apoptosis pathway. PMID: 27033411
  2. Decrease in uterine pinopodes and L-selectin ligand MECA-79 expression during uterine receptivity period by testosterone may cause failure of blastocyst implantation. PMID: 24966906
  3. E-selectin blockade similarly inhibits the migration of activated CCR4(negative) and CCR4(positive) CD4 T cells to dermal inflammation. PMID: 22664869
  4. The expression of E-selectin is significantly increased in lung tissue of ovariectomized rats, an effect prevented by estradiol but not by progesterone treatment. PMID: 20735828
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Selectin/LECAM family
Database Links
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