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| Code | CSB-E07996r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 92 | ||||
| Range % | 88-108 | |||||
| 1:10 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 89-100 | |||||
| 1:40 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 93-105 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Rat soluble E-selectin (sE-selectin) ELISA Kit is a powerful tool for immunology researchers to measure the levels of sE-selectin in a variety of sample types from Rattus norvegicus (Rat).
This ELISA kit has a detection range of 78 pg/mL to 5000 pg/mL and a sensitivity of 19.5 pg/mL, providing accurate and reliable results for even low levels of E-selectin. The assay time is 1-5 hours, and the sample volume required is only 50-100ul, making it a convenient and easy-to-use product.
The sE-selectin ELISA Kit utilizes a sandwich assay principle, where the E-selectin antigen is captured by a specific antibody and detected by a second antibody labeled with a detection reagent. The detection wavelength is 450 nm, providing a reliable and consistent readout.
This ELISA kit is suitable for a range of sample types, including serum, plasma, cell culture supernates, and tissue homogenates, making it a versatile solution for researchers in the field of immunology. It has been cited in over 9 publications, highlighting its reliability and utility in research.
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