Rat transforming growth factor α,TGF-α ELISA Kit

Code CSB-E07289r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Alternative Names
Tgfa ELISA Kit; Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha ELISA Kit; TGF-alpha ELISA Kit; EGF-like TGF ELISA Kit; ETGF ELISA Kit; TGF type 1)] ELISA Kit
Abbreviation
TGFA
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/ml-500pg/ml
Sensitivity
1.95 pg/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Transforming Growth Factor α (TGF-α) ELISA Kit is designed for the accurate and reliable detection of transforming growth factor alpha (TGF-α) in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.

The assay is based on the quantitative sandwich method and has a detection range of 7.8 pg/ml to 500 pg/ml, with a sensitivity of 1.95 pg/ml. With a quick assay time of 1-5 hours and a sample volume of 50-100ul, this kit provides an efficient and time-saving solution for researchers in the signal transduction field.

The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of TGF-α in rat samples. The Rat Transforming Growth Factor α, TGF-α ELISA Kit is based on a sandwich assay principle that provides high specificity and accuracy for the detection of this protein.

The Rat Transforming Growth Factor α (TGF-α) ELISA Kit is ideal for researchers studying signal transduction,cell migration, growth, and differentiation. Whether you are investigating the role of TGF-α in the development of cancer or its potential as a therapeutic target, this kit offers a reliable and accurate means of quantifying TGF-α levels in your samples.

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Target Background

Function
(From Uniprot)
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Gene References into Functions
  1. 5-HT stimulates DNA synthesis and proliferation in primary cultures of adult rat hepatocytes by acting via autocrine secretion of TGF-alpha induced by the serotonin 5-HT2B receptor/Gq/PLC/Ca2+ pathway. PMID: 26804134
  2. Lipopolysaccharide exposure led to a suppression of both HDAC1 and HDAC2 expression and activity, induced TGFa expression, and disrupted alveolar morphology. PMID: 24595367
  3. results support the hypothesis that FSH stimulates granulosa cell proliferation via theca TGFalpha secretion PMID: 12021046
  4. A novel mechanism for mitogenic signaling via pro-transforming growth factor alpha within hepatocyte nuclei. PMID: 12029622
  5. TGF-alpha exerts a neuroprotective action against NMDA toxicity, in which Erk1/2 activation plays a key role PMID: 12771152
  6. TGF-alpha, laminin, and fibronectin are required for ovary follicle development and maturation PMID: 14656002
  7. TGFalpha plays an autocrine role in LH dependent development and function of Leydig cells. PMID: 15353178
  8. study adds TGFalpha to the list of dysregulated cytokines present in degrading cartilage in osteoarthritis PMID: 17968906
  9. mechanical stretch promotes fetal type II cell differentiation via ectodomain shedding of HB-EGF and TGF-alpha PMID: 19237431
  10. TGF-alpha has the ability to suppress anoikis of intestinal epithelial cells, at least in part, by reverting the loss of c-Src activity and Bcl-X(L) expression induced by detachment from the ECM. PMID: 11487584

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Subcellular Location
[Transforming growth factor alpha]: Secreted, extracellular space.; [Protransforming growth factor alpha]: Cell membrane; Single-pass type I membrane protein.
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