Sheep Immunoglobulin G,IgG ELISA Kit

Code CSB-E14400Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Sheep IgG ELISA Kit was designed for the quantitative measurement of Sheep IgG protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15 μg/mL-240 μg/mL and the sensitivity is 7.5 μg/mL.

Target Name Immunoglobulin G,IgG
Abbreviation IgG
Species Ovis aries (Sheep)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 15 μg/mL-240 μg/mL
Sensitivity 7.5 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 94  
Range % 88-97  
1:200 Average % 96  
Range % 91-100  
1:400 Average % 98  
Range % 95-106  
1:800 Average % 93  
Range % 87-98  
Recovery
The recovery of sheep IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-100  
EDTA plasma (n=4) 95 92-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
240 0.182 0.185 0.184    
120 0.337 0.341 0.339    
60 0.556 0.564 0.560    
30 0.832 0.841 0.837    
15 1.297 1.313 1.305    
0 2.207 2.215 2.211    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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