Sheep Somatotropin(GH1) ELISA kit

Instructions
Code CSB-EL009407SH
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name growth hormone 1
Alternative Names GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
Abbreviation GH1
Uniprot No. P67930
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 2.5 ng/mL-40 ng/mL
Sensitivity 0.675 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep GH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 90-101  
1:2 Average % 93  
Range % 87-98  
1:4 Average % 96  
Range % 95-102  
1:8 Average % 95  
Range % 89-99  
Recovery
The recovery of sheep GH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-102  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
40 0.173 0.175 0.174    
20 0.308 0.312 0.310    
10 0.639 0.644 0.642    
5 1.401 1.408 1.405    
2.5 2.261 2.265 2.263    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
Gene References into Functions
  1. Association analyses showed that both g.498G>C and g.616G>A GH polymorphisms were significantly associated with several growth traits (at P < 0.01 or P < 0.05) in three investigated sheep breeds. PMID: 27706626
  2. The effect leptin, orexin-A, and ghrelin on the secretion of luteinizing hormone (LH) and growth hormone by adenohypophyseal explants obtained from normally fed or fasted ewes during the breeding and nonbreeding seasons. PMID: 24906931
  3. The results showed that the growth hormone genotypes are associated with estimated breeding values of growth traits. PMID: 21328104
  4. The simultaneous increase of expression of hypothalamic NPY suggests NPY involvement in the regulation of psychoemotional stress through the somatotrophic axis in the female lambs. PMID: 20675289
  5. These data are consistent with the effects of LPS on GH secretion originating through peripheral cytokine presentation to the pituitary, as well as a potential to act directly on selective populations of pituitary cells via CD14. PMID: 15899940
  6. Results show that in comparison to the product of the ovine growth hormone (GH)2-N gene copy, the postulated product of the GH2-Z gene copy, the G9R/G63S mutein, has a higher affinity to the growth hormone receptor and elevated biological activity. PMID: 17850798
  7. Results describe thet pulsatility of cerebrospinal fluid and plasma ghrelin levels in relation to growth hormone secretion and food intake in the sheep, and the effects of exogenous ghrelin on GH and feeding behavior. PMID: 18673413
  8. study has demonstrated for the first time that ghrelin differentially modulates growth hormone secretory response to growth hormone-releasing hormone according to feeding states in ruminant animals PMID: 19342191

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Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

KEGG: oas:443329

UniGene: Oar.641

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