Human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit

Product Details

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Target Name

leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4

Alternative Names

CD85 antigen-like family member K ELISA Kit; CD85k ELISA Kit; HM18 ELISA Kit; ILT-3 ELISA Kit; ILT3 ELISA Kit; Immunoglobulin-like transcript 3 ELISA Kit; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 ELISA Kit; Leukocyte immunoglobulin-like receptor 5 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 4 ELISA Kit; LILRB4 ELISA Kit; LILRB5 ELISA Kit; LIR-5 ELISA Kit; LIR5 ELISA Kit; LIRB4_HUMAN ELISA Kit; Monocyte inhibitory receptor HM18 ELISA Kit

Abbreviation

LILRB4

Uniprot No.

Q8NHJ6

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LILRB4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-98
1:2	Average %	97
	Range %	93-102
1:4	Average %	84
	Range %	81-89
1:8	Average %	88
	Range %	83-95

Recovery

The recovery of human LILRB4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-104
EDTA plasma (n=4)	92	89-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.188	2.288	2.238	2.113
1000	1.833	1.899	1.866	1.741
500	1.314	1.392	1.353	1.228
250	0.954	0.945	0.950	0.825
125	0.478	0.460	0.469	0.344
62.5	0.320	0.344	0.332	0.207
31.25	0.211	0.212	0.212	0.087
0	0.120	0.129	0.125

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LILRB4 ELISA Kit was designed for the quantitative measurement of Human LILRB4 protein in serum, plasma, cell culture supernates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Background

Function
(From Uniprot)

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Gene References into Functions

Results suggest that ILT3 played an important role in tumor progression in colorectal cancer by possible influence on CD45RO+ T cells in the tumor microenvironment. PMID: 30126665
These results suggest that tyrosine phosphorylation may be critical in FcgammaRI-dependent endocytosis/phagocytosis that may be regulated by LILRB4 by triggering dephosphorylation of key signalling proteins. PMID: 27725776
ILT3 may functionally contribute to a regulatory network controlling tumor progression by suppressing the Akt pathway. PMID: 28931525
The ILT3 PBs/PCs were suggested to be developmentally equivalent based on the simultaneous generation of these populations upon activation of memory B cells in vitro ILT3 expression was found to be induced efficiently by IL-2, while IFN-alpha effectively induced ILT3 PBs/PCs in vitro Utilizing the elevated ILT3 will support opening a new avenue for molecular markers for, pathogenic cells. PMID: 27742834
this study shows that LILRB4 might have dual inhibitory and activating functions, depending on the position of the functional tyrosine residues in its immunoreceptor tyrosine-based inhibitory motifs and/or the nature of the stimuli PMID: 28409541
LILRB4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
Identification of ILT4 as a cellular receptor for CSP C4d PMID: 26678451
ILT3Fc inhibits T cell activation and induces the generation of suppressor T cells targeting multiple inflammatory miRNA pathways. PMID: 22387553
Crystal structure of leukocyte Ig-like receptor LILRB4 (ILT3/LIR-5/CD85k): a myeloid inhibitory receptor involved in immune tolerance. PMID: 21454581
Data show that LILRB4 is a potent inhibitor of monocyte activation via FcgammaRI. PMID: 19833736
ILT3 precursor RNA is expressed and retained in nuclei of resting endothelial cells. PMID: 16433759
Our findings indicate that expression of ILT3 and ILT4 on CLL B cells represents a phenotypic abnormality that may play a role in tolerization of tumor-specific T cells. PMID: 17266150
The inhibitory effect of serum and membrane ILT3 in a humanized SCID mouse model describes an immune-escape mechanism that could contribute to impaired T cell responses in patients with cancer. PMID: 17513794
Both membrane and soluble ILT3 are proteins with potent immunosuppressive activity which are of importance for treatment of rejection, autoimmunity and cancer. PMID: 17923119
describe an immune-escape mechanism mediated by the inhibitory receptor immunoglobulin-like transcript 3 (ILT3) which may be responsible for failure of pancreatic cancer therapy PMID: 17993722
Progenitor mast cells expressed cell surface inhibitory LILRB4. Mature cord-blood-derived mast cells had detectable mRNA encoding multiple LILRs, none were expressed on the cell surface. PMID: 17998301
ILT3-Fc is a potent immunoregulatory agent that suppresses islet allograft rejection in humanized NOD/SCID mice. PMID: 18420485
15 single nucleotide polymorphisms are identified in the extracellular domain of immunoglobulin-like transcript 3 gene from healthy individuals. PMID: 18486764
Expressed on cultured osteoclast precursor cells derived from peripheral blood monocytes; could be inhibitory for osteoclast development in presence of receptor activator of NF-kappaB ligand (RANKL) and macrophage colony stimulating factor (M-CSF). PMID: 18802077
A significant association is observed between ILT3 dendritic cells in kidney transplants and donor age. PMID: 19010139
ILT3 may play a critical role in the control of inflammation. PMID: 19380766
A tryptophan-deprived environment generates monocyte-derived dendritic cells with a marked up-regulation of inhibitory receptors ILT3 and ILT4 and enhanced capacity to induce CD4+CD25+Foxp3+ regulatory T cells in an ILT3-dependent manner. PMID: 19535644

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Note=Ligand binding leads to internalization and translocation to an antigen-processing compartment.

Tissue Specificity

Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.

Database Links

HGNC: 6608

OMIM: 604821

KEGG: hsa:11006

STRING: 9606.ENSP00000270452

UniGene: Hs.67846

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-E11812h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.