Human leukocyte immunoglobulin-like receptor subfamily B member 4,LILRB4 ELISA Kit

Instructions
Code CSB-E11812h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
Alternative Names CD85 antigen-like family member K ELISA Kit; CD85k ELISA Kit; HM18 ELISA Kit; ILT-3 ELISA Kit; ILT3 ELISA Kit; Immunoglobulin-like transcript 3 ELISA Kit; Leukocyte immunoglobulin like receptor subfamily B (with TM and ITIM domains) member 4 ELISA Kit; Leukocyte immunoglobulin-like receptor 5 ELISA Kit; Leukocyte immunoglobulin-like receptor subfamily B member 4 ELISA Kit; LILRB4 ELISA Kit; LILRB5 ELISA Kit; LIR-5 ELISA Kit; LIR5 ELISA Kit; LIRB4_HUMAN ELISA Kit; Monocyte inhibitory receptor HM18 ELISA Kit
Abbreviation LILRB4
Uniprot No. Q8NHJ6
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LILRB4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-98
1:2Average %97
Range %93-102
1:4Average %84
Range %81-89
1:8Average %88
Range %83-95
Recovery
The recovery of human LILRB4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10198-104
EDTA plasma (n=4)9289-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.188 2.288 2.238 2.113
10001.833 1.899 1.866 1.741
5001.314 1.392 1.353 1.228
2500.954 0.945 0.950 0.825
1250.478 0.460 0.469 0.344
62.50.320 0.344 0.332 0.207
31.250.211 0.212 0.212 0.087
00.120 0.129 0.125
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. Involved in the down-regulation of the immune response and the development of tolerance, e.g. towards transplants. Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation. Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
Gene References into Functions
  1. Results suggest that ILT3 played an important role in tumor progression in colorectal cancer by possible influence on CD45RO+ T cells in the tumor microenvironment. PMID: 30126665
  2. These results suggest that tyrosine phosphorylation may be critical in FcgammaRI-dependent endocytosis/phagocytosis that may be regulated by LILRB4 by triggering dephosphorylation of key signalling proteins. PMID: 27725776
  3. ILT3 may functionally contribute to a regulatory network controlling tumor progression by suppressing the Akt pathway. PMID: 28931525
  4. The ILT3 PBs/PCs were suggested to be developmentally equivalent based on the simultaneous generation of these populations upon activation of memory B cells in vitro ILT3 expression was found to be induced efficiently by IL-2, while IFN-alpha effectively induced ILT3 PBs/PCs in vitro Utilizing the elevated ILT3 will support opening a new avenue for molecular markers for, pathogenic cells. PMID: 27742834
  5. this study shows that LILRB4 might have dual inhibitory and activating functions, depending on the position of the functional tyrosine residues in its immunoreceptor tyrosine-based inhibitory motifs and/or the nature of the stimuli PMID: 28409541
  6. LILRB4 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  7. Identification of ILT4 as a cellular receptor for CSP C4d PMID: 26678451
  8. ILT3Fc inhibits T cell activation and induces the generation of suppressor T cells targeting multiple inflammatory miRNA pathways. PMID: 22387553
  9. Crystal structure of leukocyte Ig-like receptor LILRB4 (ILT3/LIR-5/CD85k): a myeloid inhibitory receptor involved in immune tolerance. PMID: 21454581
  10. Data show that LILRB4 is a potent inhibitor of monocyte activation via FcgammaRI. PMID: 19833736
  11. ILT3 precursor RNA is expressed and retained in nuclei of resting endothelial cells. PMID: 16433759
  12. Our findings indicate that expression of ILT3 and ILT4 on CLL B cells represents a phenotypic abnormality that may play a role in tolerization of tumor-specific T cells. PMID: 17266150
  13. The inhibitory effect of serum and membrane ILT3 in a humanized SCID mouse model describes an immune-escape mechanism that could contribute to impaired T cell responses in patients with cancer. PMID: 17513794
  14. Both membrane and soluble ILT3 are proteins with potent immunosuppressive activity which are of importance for treatment of rejection, autoimmunity and cancer. PMID: 17923119
  15. describe an immune-escape mechanism mediated by the inhibitory receptor immunoglobulin-like transcript 3 (ILT3) which may be responsible for failure of pancreatic cancer therapy PMID: 17993722
  16. Progenitor mast cells expressed cell surface inhibitory LILRB4. Mature cord-blood-derived mast cells had detectable mRNA encoding multiple LILRs, none were expressed on the cell surface. PMID: 17998301
  17. ILT3-Fc is a potent immunoregulatory agent that suppresses islet allograft rejection in humanized NOD/SCID mice. PMID: 18420485
  18. 15 single nucleotide polymorphisms are identified in the extracellular domain of immunoglobulin-like transcript 3 gene from healthy individuals. PMID: 18486764
  19. Expressed on cultured osteoclast precursor cells derived from peripheral blood monocytes; could be inhibitory for osteoclast development in presence of receptor activator of NF-kappaB ligand (RANKL) and macrophage colony stimulating factor (M-CSF). PMID: 18802077
  20. A significant association is observed between ILT3 dendritic cells in kidney transplants and donor age. PMID: 19010139
  21. ILT3 may play a critical role in the control of inflammation. PMID: 19380766
  22. A tryptophan-deprived environment generates monocyte-derived dendritic cells with a marked up-regulation of inhibitory receptors ILT3 and ILT4 and enhanced capacity to induce CD4+CD25+Foxp3+ regulatory T cells in an ILT3-dependent manner. PMID: 19535644

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells.
Database Links

HGNC: 6608

OMIM: 604821

KEGG: hsa:11006

STRING: 9606.ENSP00000270452

UniGene: Hs.67846

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