rat Endothelin 1,ET-1 ELISA Kit

Code CSB-E06979r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name endothelin 1
Alternative Names Edn1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
Abbreviation EDN1
Uniprot No. P22388
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell lysates, tissue homogenates
Detection Range 1.25 pg/mL-80 pg/mL
Sensitivity 0.31 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %85
Range %84-91
1:2Average %96
Range %91-102
1:4Average %84
Range %81-87
1:8Average %92
Range %88-96
The recovery of rat ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)9590-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
802.085 2.086 2.086 1.951
401.540 1.649 1.595 1.460
201.111 1.156 1.134 0.999
100.718 0.750 0.734 0.599
50.407 0.405 0.406 0.271
2.50.231 0.237 0.234 0.099
1.250.181 0.173 0.177 0.042
00.129 0.140 0.135  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Target Data

Function Endothelins are endothelium-derived vasoconstrictor peptides.
Gene References into Functions
  1. Results suggest that increases in central ET-1 activity could possibly play a role in chronic estradiol-17beta-induced increase in blood pressure. PMID: 28273940
  2. liraglutide suppresses ET-1 and enhances eNOS/sGC/PKG pathways in treatment of monocrotaline-induced pulmonary arterial hypertension PMID: 27581840
  3. In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. PMID: 28508833
  4. the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETA and ETB receptors, were quantified by real-time PCR in these veins... exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETA activation PMID: 28012855
  5. tonus of the remodeled spiral artery determined by the endovascular trophoblast response to the ET1 and NO systems PMID: 27697218
  6. EDN-1 overexpressed bone marrow-derived mesenchymal stem cells showed increased proliferation and significantly increased osteogenesis potential ability than vector transfected control PMID: 28432001
  7. Activation of renal medullary P2Y2/P2Y4 receptors promotes ET-1-dependent natriuresis in ovariectomized rats. PMID: 28468962
  8. The results of this study indicated that ET1 signaling is critical in maintaining the excitability of glutamatergic neurons in the hippocampus and, thus, in modulating anxiety-like behaviors. PMID: 28302570
  9. the increased contractile response via ET-1 receptors in the ophthalmic artery after 48 hours may elicit negative retinal consequences due to a second ischemic period. PMID: 27322388
  10. ET-1-ROCK interactions contribute to decreased alveolar and vascular growth. PMID: 27760762
  11. Nur77 decreases ET-1 expression by suppressing NF-kappaB and p38 MAPK. PMID: 27765761
  12. Fermented red ginseng ameliorated endothelial dysfunction by downregulation of endothelin-1 (ET-1) and adhesion molecules in the aorta. PMID: 27322312
  13. ET system contributes to pathological neovascularization in diabetes as evidenced by restoration of functional angiogenesis by bosentan treatment and prevention of linagliptin-mediated improvement of angiogenesis in the in vitro model PMID: 26631506
  14. Linagliptin lowered plasma ET-1 levels in diabetes, and reduced ET-1-induced vascular contraction. TLR2 antagonism in diabetic basilar arteries reduced ET-1-mediated cerebrovascular dysfunction and improved endothelium-dependent vasorelaxation PMID: 26898123
  15. Lipopolysaccharides significantly promoted ET-1-induced pulmonary arterial smooth muscle cells proliferation. PMID: 27470334
  16. ET-1-mediated elevation of intracellular Ca(2+) is strongly linked to renal microvascular contraction and is crucial for ET-1-induced contraction of SMC PMID: 26682937
  17. ET-1 may be one of the upstream effectors for programmed neuronal necrosis through abnormal LIMK2 over-expression by ROCK1. PMID: 26438559
  18. Our data demonstrates that endothelin-1-mediated influx of extracellular Ca(2+) activates transient receptor potential canonical channels 1 and 6 in cerebral vascular smooth muscle cells. PMID: 25939574
  19. cerebral microvasculature is relatively insensitive to ET-1-induced functional down-regulation PMID: 25014910
  20. Study showed that store-operated ion channels Orai1 and TRPC3 contribute to endothelin/bradykinin effects in vitro and in vivo PMID: 25873305
  21. hypoxia and anoxia via activation of endothelin-1 at the critical window of heart development inhibits cardiomyocyte proliferation and decreases myocyte endowment in the developing heart PMID: 25692855
  22. in the myocardium of old rats the gene expression of endothelin-1, iNOS and TNF-alpha was increased, and the gene expression of endothelin B receptors and eNOS was reduced, compared with young rats PMID: 25446983
  23. ET-1 and ETAR are potential targets responsible for the observed synergism effect in the hypertensive atherosclerotic process through enhancement of ET-1 binding, ET-1 binding, ETAR expression, and ET-1-induced mitogenic actions in aortic VSMCs PMID: 25824048
  24. ET-1 stimulates ETA -mediated NADPH oxidase-dependent ROS generation, which inhibits endothelial NO bioavailability and contributes to ET-1-induced contraction in healthy penile arteries. PMID: 25091502
  25. ROCK-2 inhibition restores ET-1-mediated NO production after the LPS pretreatment, in part, through an increase in actin depolymerization PMID: 25243430
  26. Salt or water loading increased ET-1 mRNA in inner medullary collecting duct PMID: 25587122
  27. Data show an inappropriate secretion of endothelin 1 (ET-1) in obese animals with a parallel DNA damage in their lungs. PMID: 25517973
  28. High glucose induced ET-1 production that mediated the EMT induced by high glucose in renal tubular epithelial cells, and HIF-1alpha acted as the upstream signal to regulate ET-1. PMID: 24447812
  29. We conclude that enhanced ET-1 signaling is part of a pathologic mechanism associated with adverse cerebrovascular outcomes of obstructive sleep apnea ( PMID: 25425077
  30. Restriction in cerebral blood flow was observed with upregulation of endothelin-1 in cerebral ischemia. PMID: 25228257
  31. These findings identify a novel mechanistic interaction between the ET-1/ETB receptor axis and CX3CL1/CX3CR1 in mediating pulmonary angiogenesis and vascular monocyte accumulation in experimental HPS. PMID: 24731444
  32. The combination therapy prevented pulmonary artery injury and angiogenesis of the arteries by reducing the level of ET-1 and promoting the level of 6-keto-PGF1alpha in the blood PMID: 24854661
  33. Endothelin-1 and endothelin-2 initiate and maintain contractile responses by different mechanisms in rat mesenteric and cerebral arteries. PMID: 23941276
  34. Ang II enhances ET-1-induced vasoconstriction by upregulating ETAR expression and ET-1/ETAR binding, which may be because of the AngII/Ang II receptor pathways and the activation of PKC or ERK. PMID: 25088996
  35. Altered pulmonary vascular reactivity in hypertension may be related to a loss of endothelial buffering of vasoconstriction and decreased leptin-induced vasodilation in conditions of increased endothelin-1. PMID: 24499246
  36. The insulin/endothelin-1 vasoconstrictor pathway is more active in GFA than in SFA. PMID: 23995100
  37. These results indicated that apocynin alleviated CIH-induced hypertension by inhibiting NADPH oxidase, further leading to the reduced vasoconstrictor ET-1 level and oxidative stress. PMID: 23592126
  38. COX-2 and NFATc3 signaling are involved in ET-1-induced hypertrophy of neonatal rat cardiomyocytes. PMID: 24291639
  39. These findings support the hypothesis that vascular endoplasmic reticulum stress-mediated activation of ET-1 may be an underlying cause of impaired vasomotor responsiveness to insulin and endothelial dysfunction. PMID: 24304569
  40. ET-1 induces a glycolytic switch in pulmonary arterial endothelial cells via the redistribution of uncoupled eNOS. PMID: 24392990
  41. Endothelin-1 has a role in activating ERK1/2 via transactivation of platelet-derived growth factor receptor in rat L6 myoblasts PMID: 24735959
  42. Levels of ET-1 peptide increase in kidney tissues after lipopolysaccharide (LPS) administration time-dependently with a rise in plasma ET-1 concentration, compared to control rats. PMID: 24641950
  43. ET-1-induced cardiomyocyte hypertrophy is mediated through peptidylprolyl cis/trans isomerase (Pin1) activation. PMID: 24657892
  44. Status epilepticus induces vasogenic edema via TNF-alpha/endothelin-1-mediated two different pathways. PMID: 24040253
  45. CIH induced increased ET-1. PMID: 23662699
  46. NO, PGI2 and ET-1 expression are regulated by 17beta-estradiol in pulmonary hypertension PMID: 22936708
  47. ET-1 enhances the phosphorylation of epidermal growth factor receptor (EGFR) in cultured vascular smooth muscle cells. PMID: 23537435
  48. The increased responsiveness to ET-1 induced by intermittent hypoxia in pulmonary arteries of rats was due to increased expression of ETA receptors PMID: 23555567
  49. Epididymal fat and soleus muscles from rat were incubated with ADM at concentration of 100 nM for the study of the gene expression and secretion of tumour necrosis factor (TNF-alpha), EDN-1, leptin, adiponectin, interleukin 1beta (IL-1beta), and IL-6. PMID: 22956308
  50. In conclusion, the cardioprotective effects of EGb761 on markers of ADR-induced acute cardiotoxicity appeared to have been mediated by the regulation of inflammatory and vasoactive mediators, as well as the inhibition of membrane lipid peroxidation. PMID: 23898483
  51. C-terminus of ETA/ETB receptors regulate endothelin-1 signal transmission. PMID: 23436727
  52. HIF-1alpha, ET-1 and iNOS are all involved in the occurrence and development of hypoxic pulmonary hypertension (HPH) in neonatal rats. PMID: 23428131
  53. ET-1 is a factor regulating astrocytic vascular endothelial growth factor (VEGF)-A and angiopoietin (ANG)-1. PMID: 22927341
  54. Silencing of brain klotho potentiated and expedited cold-induced elevation of blood pressure by upregulation of endothelin-1 and the subsequent activation of the sympathetic nervous system. PMID: 20086041
  55. responsiveness to ET-1 declines with age in retinal microvasculature. In males, this is likely mediated by age-related changes in the ET(A) receptor signaling pathway. PMID: 22906761
  56. Endothelin-1 acts in an intracrine fashion on endolysosomal ET(B) to induce nitric oxide formation, thus modulating endothelial function PMID: 23086942
  57. ET-1 and NO mediate myocardial dysfunction in isolated hearts and decrease [Ca2+]i transients in cardiac myocytes from LPS-treated rats. PMID: 21189559
  58. ET-1 and ET-2 cause long-lasting arterial contractions with comparable sensitivities and maximal responses PMID: 22406300
  59. These results suggest that NF-kappaB/ET-1/ETA receptor-mediated actions are responsible for the increased susceptibility to DOCA-salt induced renal injuries in the case of reduced NO production PMID: 22569294
  60. ET-1-induced cardiac hypertrophy and C-terminal domain phosphorylation level are functionally regulated by transcription factor IIF-interacting CTD phosphatase1 PMID: 22569295
  61. These data provide evidence that ET-1 directly increases glomerular permeability to albumin and that albuminuria is prevented by increased proximal tubule albumin uptake in the rat PMID: 22727794
  62. Excessive vasoconstrictive effects of ET-1 via ETA and upregulation of iNOS, are partly responsible for the impaired recovery of renal function following ischemia in diabetic rats. PMID: 22365956
  63. ET-1-mediated basilar artery dysfunction may contribute to neurovascular damage after stroke PMID: 22365958
  64. Chemerin increases contractile responses to PE and ET-1 via ERK1/2 activation PMID: 22521290
  65. Endothelin-1 induces hypoxia inducible factor 1a expression in pulmonary artery smooth muscle cells. PMID: 23041290
  66. Endothelin-1 may play an important role in neurogenic pulmonary edema. PMID: 21192421
  67. HIF-1 and c-Src mediate increased glucose uptake induced by endothelin-1 and connexin43 in astrocytes PMID: 22384254
  68. Intermittent hypoxia-induced carotid baroreceptor dysfunction involves reactive oxygen species-dependent upregulation of endothelin-1 signaling in the carotid sinus region. PMID: 22016368
  69. L-arginine prevents up-regulation of endothelin-1 during acute myocardial ischemia/reperfusion injury. PMID: 22153010
  70. Atf3 (activating transcription factor 3), Egr1 (early growth response 1) and Ptgs2 (prostaglandin-endoperoxide synthase 2) are rapidly and transiently up-regulated by endothelin-1 in cardiomyocytes. PMID: 22390138
  71. Furthermore, IFNgamma reduced JNK phosphorylation, which tightly was associated with decreased phosphorylation of downstream factors c-Jun and Smad3 and decreased binding activity of c-Jun and Smad3 in the preprpET-1 promoter. PMID: 22301113
  72. Hyperleptinemia increase hepatic endocannabinoids production, promote hepatic fibrogenesis, enhance the hepatic vasoconstrictive response to endothelin-1, and aggravate hepatic microcirculatory dysfunction PMID: 22183953
  73. Both ET-1 activation of ET(A)/NADPH oxidase pathway and diminished antioxidants critically contribute to epithelial progenitor cells reduction and dysfunction via increased oxidative stress in salt-sensitive hypertension. PMID: 22431579
  74. Endogenous ET-1 aggravates hypoxia-induced cardiomyocyte apoptosis and this effect is mediated through ET(A) receptor-dependent pathways. PMID: 21162225
  75. VEGF and ET-1 participate the muscularization of pulmonary vessels during hypoxia and play an important role in the process of hypoxic pulmonary hypertension in rats. PMID: 21186584
  76. Endothelin-1 elevation is not only a conserved phenomenon in a rat traumatic brain injury (TBI) model, but it is a likely target for understanding the observed enhanced vascular response to TBI. PMID: 21801588
  77. Although the pressor effect of chronic central endothelin-1 is similar to that reported with acute endothelin-1, plasma vasopressin levels do not increase, at least in part, due to downregulation of hypothalamic vasopressin gene expression. PMID: 21801591
  78. An important role for astrocytes in mediating the actions of ETs on the magnocellular neurosecretory system. PMID: 22007724
  79. Endothelin-1 induces both a transcriptional and posttranslational activation of the three major arms of the unfolded protein response. PMID: 21778413
  80. Chronic intermittent hypoxia produces a transient 2-fold increase of ET-1 immunoreactivity in the carotid body at 7 days. PMID: 21555119
  81. Pravastatin down-regulated ET-1 mRNA expression, as well as ET-1 synthesis and release induced by aldosterone in a process specifically related to mevalonate in cardiac fibroblasts. PMID: 21162281
  82. ET-1 and VEGF play important roles in the pathogenesis of hypoxic pulmonary hypertension. PMID: 21180137
  83. Endothelin-1 may participate in the instant decline of cardiac function of rats at the early stage of severe burn, and plays an important role in the instant myocardial damage after injury. PMID: 20510028
  84. PKC stimulates collecting duct ET-1 synthesis via transcriptional activation of the ET-1 promoter PMID: 21818684
  85. Interaction between ET-1 and adrenomedullin indicates their involvement in a local, fine-tuning regulatory system (together with LH and prolactin) for progesterone production in corpora lutea throughout pregnancy. PMID: 21824440
  86. our findings suggest that endothelial ET-1 expression may contribute to status epilepticus-induced vasogenic edema formation via brain-blood barrier disruption at AQP4/MCP-1 independent manners PMID: 21742016
  87. ET-1 acts as an anti-apoptotic factor on olfactory sensory neurons, directly through ETB and indirectly by limiting non neuronal cells death through ETA receptors. PMID: 21035524
  88. Endothelin 1 is involved in the long-term regulation of tyrosine hydroxylase, leading to an increase in catecholaminergic activity implicated in diverse pathologies involving the olfactory bulbs. PMID: 21129429
  89. These data indicate that a decrease in medullary production of ET-1 in the Dahl salt-sensitive (DS) rat could play an important role in the development of salt-sensitive hypertension observed in the DS rat. PMID: 21613578
  90. adult astrocytes that ET-1 production is driven by calcium influx through stretch-activated ion channels (SACs) and the ET-1 production correlates with cell proliferation. PMID: 21640709
  91. Ulinastatin may protect myocardium from the damage resulted from sepsis in a rat model, probably by lowering expressions of cTnI, TNF-alpha and ET-1. PMID: 20594472
  92. Endothelin-1 markedly decreased ABCG1 levels via endothelin type A and B receptors and activation of the phosphatidylinositol 3-kinase pathway. Endothelin-1 may have also enhanced ABCG1's degradation. PMID: 21520072
  93. The role of ET-1 and associated signaling pathways in leiomyoma pathology. PMID: 21084714
  94. Data show that TNFalpha stimulates activated stellate cells to produce ET-1 via a novel IKK-JNK-dependent signaling pathway. PMID: 21216243
  95. ET-1 augments O-GlcNAcylation and this modification contributes to increased vascular contractile responses via activation of the RhoA/Rho-kinase pathway PMID: 20978008
  96. Data show that synthetic FXR agonist significantly inhibited the HSC transdifferentiation, which was associated with an inhibition of of ET-1 expression. PMID: 21085652
  97. The enhanced levels of endogenous ET-1 in VSMC from SHR increase oxidative stress, which contribute to the enhanced expression of Gialpha proteins. PMID: 20959139
  98. ET may play an important role in the process of the cochlear microcirculation disorder caused by noise. PMID: 16200958
  99. Data indicate that ERK5 signaling regulates glucose-induced ET-1 expression in diabetes. PMID: 20628425
  100. Prolonged pain tachyphylaxis induced by endothelin-1 is not due to reduced responsiveness of the central nervous system, but rather depends on the functional sensitivity or availability of peripheral ET(A) receptors. PMID: 20628433

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Subcellular Location Secreted
Protein Families Endothelin/sarafotoxin family
Database Links

KEGG: rno:24323

STRING: 10116.ENSRNOP00000019361

UniGene: Rn.10918

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