Recombinant Human Plasminogen activator inhibitor 2 protein(SERPINB2) (Active)

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Code CSB-AP000151HU
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Product Details

Purity >95% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biologically active was determined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37 °C. Half maximal inhibition against 1.0 μg/ml of single chain tPA was at a concentration of 1.0 μg/ml.
Target Names SERPINB2
Uniprot No. P05120
Research Area Immunology
Alternative Names HsT1201; Monocyte Arg serpin ; Monocyte Arg-serpin; Monocyte Arginine-serpin; Monocyte-derived plasminogen activator inhibitor; PAI; PAI-2; PAI2 ; PAI2_HUMAN; Placental plasminogen activator inhibitor; PLANH2 ; Plasminogen activator inhibitor 2; plasminogen activator inhibitor; type 2; plasminogen activator inhibitor; type II (arginine-serpin); serine (or cysteine) proteinase inhibitor; clade B (ovalbumin); member 2; Serpin B2; serpin peptidase inhibitor; clade B (ovalbumin); member 2; Serpinb2; Urokinase inhibitor
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-415aa
Complete Sequence MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP
Mol. Weight 46.6 kDa
Protein Length Full Length
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM Cysteine, with 5 % Trehalose
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.
Gene References into Functions
  1. miR-15a was identified as a downregulated miRNA in CCFs. Moreover, PAI-2 was identified as a novel target gene of miR-15a. PMID: 29347950
  2. Single nucleotide polymorphisms in ICAM1 (rs1799969) and SERPINB2 (rs6103) genes were found to be protective against thalidomide-induced peripheral neuropathy (TiPN). In children with inflammatory bowel disease, TiPN is common but mild and generally reversible. Cumulative dose seems to be the most relevant risk factor, whereas polymorphisms in genes involved in neuronal inflammation may be protective. PMID: 28817461
  3. serpinB2 bound to and stabilized p21 to mediate senescence in a proteasome-independent manner, indicating that serpinB2 has a direct role in senescence. Thus, this study reveals a unique mechanism by which serpinB2 maintains senescence through stabilization of p21 protein levels. PMID: 28794016
  4. findings demonstrate the prospective role of SerpinB2 as a novel biomarker for acquired gefitinib resistance and a potential target for non-small cell lung cancer treatment. PMID: 27558531
  5. SerpinB2 promotes miR-200c/141 cluster overexpression-induced breast cancer cell metastasis PMID: 28427146
  6. PAI-2 likely plays a key role in cardiovascular disease through multiple pathophysiologic processes including racial dependency, platelet clot initiation and propagation, oxidative stress, inflammation effects on HDL metabolism and coagulation PMID: 27174532
  7. This study is the first to associate enhancer RNAs with SERPINB2 and is the first demonstration of acquisition of NELF binding by enhancer RNAs on chromatin. PMID: 28578223
  8. This study establishes a novel role for SerpinB2 in the stromal compartment in pancreatic ductal adenocarcinoma invasion through regulation of stromal remodelling PMID: 28346421
  9. The variant of PAI-2 gene was associated with coronary artery disease and recurrent coronary event risk in Chinese Han population, in Xinjiang. PMID: 26573152
  10. SerpinB2 plays an important role in proteostas PMID: 26083412
  11. Polymorphisms in this fibrinolytic system gene are associated with recurrent spontaneous abortion in Sinhalese women, probably through impaired implantation. PMID: 25444509
  12. PAI-2 was up-regulated in tensioned keloid fibroblasts and normal fibroblasts, but more so in keloid cells. Knockdown of PAI2 reduced cell proliferation in fibroblasts under tension. PMID: 25046655
  13. Soluble guanylate cyclase activators might alleviate or reverse vascular remodeling in pulmonary hypertension through the up-regulation of PAI-2. PMID: 25704756
  14. We found no association between allele frequency and risk of multiples sclerosis for any single nucleotide polymorphism investigated for serpinb2 PMID: 23897640
  15. A role for SERPINB2 in patients with head and neck squamous cell carcinoma. PMID: 23661500
  16. Plasminogen activator inhibitor-2 polymorphism associates with recurrent coronary event risk in patients with high HDL and C-reactive protein levels. PMID: 23874812
  17. PSMB1 is part of the transcriptional machinery required for gastrin stimulated expression of PAI-2 and Reg1. PMID: 23544109
  18. SerpinB2 can be induced by lentiviral infection in vivo. PMID: 23460840
  19. Low expression of PAI-2 serves as a novel marker of portal vein tumor embolism and poor prognosis for hepatocellular cancer. PMID: 23188538
  20. Increased expression of SerpinB2 by an inflammatory stimulus is sufficient to generate structures that resemble secretory vesicles. PMID: 23474086
  21. PAI-2 expression may be negatively associated with the invasive potential of hepatocellular carcinoma (HCC). PMID: 23527801
  22. a model for the transcriptional control of the human PAI-2 gene PMID: 22334683
  23. Data suggests that PAI-2, in endothelial cells induced with inflammatory stimuli, can inhibit proteasome and thus tilt the balance favoring proapoptotic signaling. PMID: 21976669
  24. High PAI-2 expression is associated with poor treatment response in colorectal carcinoma. PMID: 21744990
  25. Plasminogen activator inhibitor-2 (PAI-2) secreted from activated mast cells induces alpha-smooth muscle actin (alpha-SMA) expression in dermal fibroblasts PMID: 21477997
  26. Our results suggest that P. intermedia may contribute to periodontal tissue destruction by upregulating tPA and PAI-2 expression in hPDL cells via multiple signaling pathways. PMID: 21314733
  27. The decrease in plasma PAI-2 observed in preeclampsia does not precede the clinical onset of the disease. PMID: 20205627
  28. Results describe periodontal treatment effects on gingival crevicular fluid (GCF) interleukin-6 (IL-6), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor-2 (PAI-2), albumin levels in type 2 diabetic patients. PMID: 20845058
  29. HPV-transformed CaSki cells express high levels of SerpinB2, with cellular distribution, glycosylation, secretion, cleavage, induction and urokinase binding similar to that for primary cells; SerpinB2 efficiently binds the proteasomal subunit member beta1 PMID: 20974129
  30. PAI-2 has a role in promoting the differentiation of human epidermal keratinocytes. PMID: 20494554
  31. It is feasible to detect fetal trisomy 18 non-invasively by maternal plasma SERPINB2 RNA-SNP analysis provided that sufficient quantities of plasma samples are used. PMID: 19650060
  32. regulator of monocyte proliferation and differentiation PMID: 11929770
  33. plasminogen activator inhibitor type 2: potential prognostic factor for endometrial carcinomas PMID: 11949838
  34. Identification of plasminogen activator inhibitor-2 as a gastrin-regulated gene PMID: 12105855
  35. post-transcriptional regulation of the PAI-2 gene is modulated by tristetrapolin PMID: 12578825
  36. Data suggest that the CD-loop functions as a redox-sensitive switch that converts plasminogen activator inhibitor type 2 (PAI-2) between an active stable monomeric and a polymerogenic conformation. PMID: 12682008
  37. These results indicate that PAI-2 may enhance Rb's tumor suppressor activity and suggest a potential therapeutic role for PAI-2 against HPV-transformed lesions. PMID: 12944478
  38. evidence that a polymorphism of the PAI-2 gene is associated with an increased risk of myocardial infarction PMID: 14653443
  39. PAI-2 has a role in scleroderma, as seen by its expression in fibroblasts PMID: 15500643
  40. PAI-2 expression has a potent suppressive effect on human papillomavirus type 18 oncogene transcription mediated by Rb and LIP, a finding with potential implications for prognosis and treatment of human papillomavirus-transformed lesions. PMID: 15767426
  41. PAI2 is present in normal conjunctiva. PMID: 15887231
  42. investigated the unique mousetrap inhibition mechanism of serpins through saturation mutagenesis of the P8 residue for PAI-2 PMID: 16214170
  43. the urokinase/PAI-2 complex is a new high affinity ligand for the endocytosis receptor low density lipoprotein receptor-related protein PMID: 16459332
  44. SerpinB2 is a potentially important inducible host factor that significantly promotes HIV-1 replication PMID: 16923810
  45. in patients with complex congenital malformations amniotic fluid levels of plasminogen activator inhibitor type 2(PAI2) PMID: 17141398
  46. Data indicate that this family did not follow the Mendelian inheritance pattern; the Ser(413)/Ser genotype in 60% of the affected members might increase the risk for autoimmune syndromes such as anti-phospholipid syndrome or systemic lupus erythematosus. PMID: 17657675
  47. PAI-2 is able to inhibit and clear urokinase plasminogen activator activity without initiating mitogenic signalling events through the very-low-density-lipoprotein receptor (VLDLr). PMID: 17696882
  48. Type 2 diabetes in this study seems not to increase gingival crevicular fluid levels of the evaluated inflammatory mediators PAI2. PMID: 18472001
  49. SerpinB2 is a cell survival factor that modulates Rb repression of proapoptotic signal transduction PMID: 18632617
  50. PAI-2 can inhibit cell-bound tPA activity in vitro and thus prevent plasmin formation PMID: 18690354

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Subcellular Location Cytoplasm, Secreted, extracellular space
Protein Families Serpin family, Ov-serpin subfamily
Database Links

HGNC: 8584

OMIM: 173390

KEGG: hsa:5055

STRING: 9606.ENSP00000299502

UniGene: Hs.594481

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