Product Details

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

Less than 0.1 EU/ug as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.

Target Names

GDF7

Uniprot No.

Q7Z4P5

Research Area

Signal Transduction

Alternative Names

bmp12; bone morphogenetic protein 12; GDF-7; Gdf7; GDF7_HUMAN; growth differentiation factor 7; Growth/differentiation factor 7

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

322-450aa

Complete Sequence

TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR

Mol. Weight

14.0 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

GDF7 (BMP12) plays a critical role in tendon and ligament differentiation, making it a key tool for studying musculoskeletal and connective tissue biology. This tag-free recombinant human GDF7, covering the full mature protein (residues 322–450), demonstrates an ED50 of less than 1.0 μg/ml in ATDC5 alkaline phosphatase induction assays, corresponding to a specific activity exceeding 1000 IU/mg—confirming robust BMP receptor engagement suitable for osteogenic and chondrogenic differentiation studies, receptor-ligand binding assays via SPR or ELISA, and in vivo regenerative medicine models. E. coli expression yields a non-glycosylated homodimer that provides a defined, consistent baseline for mechanistic signal transduction experiments without glycosylation-related variability. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 0.1 EU/μg satisfies the criteria typical for cell-based differentiation assays and supports use as a reliable ELISA standard for antibody characterization workflows.

Target Background

Function

May play an active role in the motor area of the primate neocortex.

Gene References into Functions

BMP-12 activates tenogenic pathway in human adipose stem cells. PMID: 28214472
The Barrett-associated variants at GDF7 and TBX5 also increase esophageal adenocarcinoma risk. PMID: 26783083
closest protein-coding genes were respectively GDF7 (rs3072), which encodes a ligand in the bone morphogenetic protein pathway, and TBX5 (rs2701108), which encodes a transcription factor that regulates esophageal and cardiac development PMID: 25447851
BMP12 induces tenogenic differentiation of adipose-derived stromal cells via the Smad1/5/8 pathway. PMID: 24155967
studies show that even though tenogenic (BMP 12 and BMP 13) and osteogenic (BMP2) BMPs bind the same receptors with high affinity they signal much differently and result in differential activation of osteogenic and tenogenic markers PMID: 21702718
induces ligamentogenic differentiation in mesenchymal progenitors PMID: 20334610
stimulates expression of both chondrogenic and osteoblstic markers in pluripotent mesenchymal C3H10T1/2 cells. PMID: 20102312
In mouse, the expression of Gdf7 in roof plate cells is required for the fidelity of commissural axon growth. PMID: 12741987

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Subcellular Location

Secreted.

Protein Families

TGF-beta family

Database Links

HGNC: 4222

OMIM: 604651

KEGG: hsa:151449

STRING: 9606.ENSP00000272224

UniGene: Hs.447688

Code	CSB-AP005961HU
Abbreviation	Recombinant Human GDF7 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Growth/differentiation factor 7 (GDF7)(Active)

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