Recombinant Human Platelet-derived growth factor subunit B protein(PDGFB) (Active)

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Code CSB-AP002631HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of >3.3x105 IU/mg.
Target Names PDGFB
Uniprot No. P01127
Research Area Cancer
Alternative Names Becaplermin; c sis; FLJ12858; Oncogene SIS; PDGF 2; PDGF B chain; PDGF Beta; PDGF subunit B; PDGF-2; PDGF2; Pdgfb; PDGFB_HUMAN; Platelet derived growth factor 2; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide (oncogene SIS); Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog); Platelet derived growth factor beta polypeptide; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Platelet-derived growth factor subunit B; Proto-oncogene c-Sis; Simian sarcoma viral (v sis) oncogene homolog; SIS; SSV; v sis platelet derived growth factor beta polypeptide
Species Homo sapiens (Human)
Source E.Coli
Expression Region 82-190aa
Mol. Weight 12.4 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin
Gene References into Functions
  1. we identified two potential susceptibility loci for PC risk, which are located in PDGFB at 22q13.1 PMID: 29168174
  2. Treating HepG2 cells with hepatotoxicants resulted in a significant increase in mRNA expression of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta (TGFbeta). PMID: 29558627
  3. The morphology and immunophenotype of all 6 cases was analogous to those with the canonical COL1A1-PDGFB fusion; none of the cases showed fibrosarcomatous transformation. This study illustrates that the COL6A3-PDGFD fusion product is rare in dermatofibrosarcoma protuberans, and associated with an apparent predilection for breast PMID: 30014607
  4. PDGF-B expression was found in ovarian tumor microvessels in 72% of cases. High expression of PDGF in pericapillary cells was strongly associated with high expression of this marker in cancer cells. Significant correlations between PDGF-B and nestin expression in malignant tumor microvessels were also found. PMID: 28397199
  5. SOX7 transcription factor mediates PDGF-BB-induced IL-33 expression. PMID: 27150562
  6. High expression of PDGF-BB may be involved in the pathogenesis of Graves' disease. CCR2-positive macrophages may induce the expression of PDGF-BB through HIF-1alpha signal. PMID: 29319128
  7. rhPDGF-BB promoted the proliferation of hADSCs via miR-363/PI3K/Akt pathway, indicating that rhPDGF-BB combined with ADSCs could treat Achilles tendinitis via miR-363/PI3K/Akt pathway. PMID: 28766166
  8. This study identifies the mbPDGF-BB in cell derived extracellular vesicles as a relevant mediator of diabetes-associated vascular smooth muscle cell resistance to apoptosis. PMID: 29386225
  9. This review showed that PDGFB was one of the common gene involved included with brain calcification. PMID: 28162874
  10. Rare Skin Tumor Dermatofibrosarcoma Protuberans (DFSP) is characterized by the translocation of the PDGFB gene to the collagen 1A1 gene. PMID: 28940884
  11. Transglutaminase type 2 affects cell migration through post-translational modification of PDGF-BB. PMID: 27633721
  12. PDGF-B rs1800818 polymorphism might play a role in mediating the susceptibility to severe fever with thrombocytopenia syndrome in Chinese individuals. PMID: 27147565
  13. PDAP-1 as an effecter of PDGF signaling in glioma cells PMID: 27448842
  14. High PDGFB expression is associated with gastric cancer. PMID: 28423550
  15. We observed that regenerating and necrotic muscle fibers in muscle biopsy samples from Duchenne muscular dystrophy patients expressed PDGF-BB. PMID: 28618254
  16. Elevated PDGFB expression was noted in 29% of patients with papillary renal cell carcinoma. PMID: 27989785
  17. high-throughput affinity plasma proteomic profiling is a valuable research strategy to identify potential candidate biomarkers for thrombosis-related disorders, and our study suggests a novel association of PDGFB plasma levels with venous thromboembolism. PMID: 27742707
  18. High PDGFB expression is associated with glioma. PMID: 26951930
  19. A cell-autonomous positive-signaling circuit is associated with the PDGF-NO-ID4-regulatory axis in glioblastoma cells. PMID: 28327358
  20. SphK1 is regulated by PDGF-BB in pulmonary artery smooth muscle cells via the transcription factor Egr-1, promoting cell proliferation. PMID: 27099350
  21. PDGF-BB regulates the proliferation and differentiation of human melanocytes in a differentiation-stage-specific manner. PMID: 27289338
  22. this study shows that the expression of PDGFB is significantly downregulated in keloid fibroblasts compared to normal human fibroblasts PMID: 27465069
  23. this study demonstrated that knockdown of SCARA5 inhibits PDGFBBinduced HASMC proliferation and migration through suppression of the PDGF signaling pathway. PMID: 27035566
  24. The simultaneous action of PDGF-B/PDGFRbeta and VEGF165b on the same type of receptor may explain the resistance to antiangiogenic therapy, which depends on the degree of modulation of PDGFRbeta phosphorylation. PMID: 27127135
  25. The expression of angiogenesis markers VEGF-A, VEGFR, PDGFbetabeta, PDGFR, CCND1 and CA9 was assessed by immunohistochemistry and correlated with overall survival and progression-free survival in patients with renal cell carcinoma undergoing therapy with Sunitinib, but no correlation was found between expression of angiogenesis markers and clinical outcome. PMID: 28011500
  26. ore than 65% of cases had PDGF-BB mRNA amplification, confirming immunohistochemical results. We herein validated PDGF-BB as a potential therapeutic and prognostic tool of ovarian cancer aggressiveness. PMID: 27807074
  27. Case Report: congenital atrophic dermatofibrosarcoma protuberans with COL1A1-PDGFB rearrangement. PMID: 26932148
  28. Placental endothelial cell-derived PDGF-BB recruits human placental multipotent mesenchymal stromal cells involved in vascular development via PDGFR-beta/STAT3 activation PMID: 26353894
  29. Suggest that PDGF-B signaling may play a role in endothelial and cardiomyocyte recovery from ischemia reperfusion injury after heart transplantation. PMID: 26371596
  30. Lessons from SLC20A2, PDGFB, and PDGFRB mutation carriers. Three causative genes have been identified: SLC20A2, PDGFRB and, recently, PDGFB, whose associated phenotype has not yet been extensively studied. PMID: 26129893
  31. loss-of-function mutations in PDGFB or PDGFRB cause Primary Familial Brain Calcification. PMID: 26599395
  32. Three factor model revealed significant gene-gene interaction for PDGFB +286A>G, PDGFB +1135A>C and HER2 Ile165Val SNPs with GBC. Protein-protein interaction showed significant association of PDGFB and HER2 with EGFR receptor signaling pathway. PMID: 26320430
  33. Regulation of Hyaluronan (HA) Metabolism Mediated by HYBID (Hyaluronan-binding Protein Involved in HA Depolymerization, KIAA1199) and HA Synthases in Growth Factor-stimulated Fibroblasts. PMID: 26518873
  34. Electron microscopy structure of PDGFRB [a full-length human platelet-derived growth factor receptor], in complex with its ligand PDGF-B. PMID: 26463591
  35. TM expression in corneal epithelium was modulated during the corneal wound healing process, and may be regulated by PDGF-BB. In addition, rTMD23 has therapeutic potential in corneal injury PMID: 25816372
  36. Our results demonstrated that PDGF-B tumor growth and progression in clear cell renal cell carcinoma PMID: 25766258
  37. PDGFB and IL18R1 represent plausible candidates for studying the pathophysiology of these disorders in the context of TLR4 activation PMID: 25327457
  38. TAZ promotes neuroblastoma cell proliferation and tumorigenicity through up-regulating the expression of PDGF-beta genes. PMID: 25940705
  39. Phloretin inhibits PDGF-BB-induced thoracic aorta smooth muscle cell proliferation and migration. PMID: 25945863
  40. COL1A1-PDGFbeta translocation is specific to dermatofibrosarcoma protuberans. Platelet-derived growth factor may have acted in an autocrine manner to cause cell division, which may have led to the development of dermatofibrosarcoma protuberans PMID: 25924890
  41. the present study, demonstrated for the first time, to the best of our knowledge, that ligustrazine downregulated PDGF-BB-induced VSMC proliferation and migration partly, at least, through inhibiting the activation of the ERK and P38 MAPK signaling. PMID: 25738255
  42. cytoplasmic expression of VEGF, VEGFR2, PDGF-B, and PDGFR-beta in RCC tumour cells is different in various pathologic stage and cell type. Notably, VEGF and PDGF-B expression are higher in papillary than in clear cell renal cell carcinoma. PMID: 25550804
  43. COL1A1-PDGFB is a useful and accurate tool in diagnosing DFSP[ Dermatofibrosarcoma protuberans ] in Koreans. PMID: 25683993
  44. Significant diurnal variations in platelet counts and TGF-b1 and PDGF-BB levels were not observed in platelet-rich plasma. PMID: 24878758
  45. Report lowered PDGF-BB levels in acute pancreatitis and increased PDGF-BB levels in chronic pancreatitis. PMID: 25278706
  46. rhPDGF-BB delivery on a collagen scaffold enhanced cellular proliferation and angiogenesis during the early phase of healing after rotator cuff repair. PMID: 25349036
  47. The mutation of PDGFB cause primary familial brain calcifications. PMID: 25212438
  48. Gain of PDGFB is associated with response to therapy in metastatic renal cell carcinoma. PMID: 24524969
  49. These results suggest that PDGF-BB promotes pulmonary artery smooth muscle cells proliferation and survival, which is likely to be mediated via the JNK pathway. PMID: 24804810
  50. PDGFB hypomethylation is a favourable prognostic biomarker in primary myelofibrosis. PMID: 25498506
  51. Data show that the CXC chemokine receptor 4 (CXCR4) antagonist AMD 3100 disrupted the stromal cell-derived factor 1 (SDF-1alpha)/CXCR4 axis and decreases platelet-derived growth factor B (PDGF-B) protein expression in vivo in two xenograft Ewing tumor models. PMID: 24282276
  52. Antiplatelet therapy or selective inhibition of PDGFB might reduce biliary fibrosis in patients with liver disease. PMID: 25173753
  53. Coexpression of both PDGF-BB and VEGF-C was associated with lymphangiogenesis and poor prognosis in NSCLC. PMID: 24972450
  54. This study showed the importance of the methylation status of the PDGF-B gene promoter, and suggests that the epigenetic regulation of the PDGF-B gene may serve as a potential therapeutic target for the inhibition of glioma proliferation. PMID: 24502980
  55. After treatment with recombinant human PDGF-BB (rhPDGF-BB), diabetic Bone marrow stromal cells demonstrated enhanced cell proliferation and osteogenic differentiation based on increased expressions of osteogenic genes PMID: 24605332
  56. These findings thus implicate a novel role of PDGF-BB in the migration of pericytes, resulting in loss of pericyte coverage from the endothelium with a subsequent breach of the blood-brain barrier PMID: 25164676
  57. The data indicate a dual role of Lhx2 during EMT and tumor progression: by inducing the expression of PDGF-B, Lhx2 provokes an autocrine PDGF-B/PDGFRbeta loop required for cell migration, invasion and metastatic dissemination PMID: 24423492
  58. The Semaphorin 4D-Plexin-B1-RhoA signaling axis recruits pericytes and regulates vascular permeability through endothelial production of PDGF-B and ANGPTL4. PMID: 24114199
  59. PDGF-BB significantly inhibits the osteogenic differentiation of mesenchymal stem cells and miR-138 gene transcription is controlled by PDGF-BB signaling. PMID: 24792185
  60. The comparison between the cytokine profiles of ET and PV patients showed a statistically significant increase of IL-4, IL-8, GM-CSF, interferon -gamma, MCP-1, PDGF-BB, and VEGF in the ET group PMID: 24463275
  61. The present study developed an efficient strategy for differentiating human hair follicle stem cells into contractile smooth muscle cells by stimulation with TGF-beta1 and PDGF-BB. PMID: 24084832
  62. Found PDGF-bb protein down-regulated in the serum of patients with colorectal cancer compared with the control group. Thus, PDGF-bb might play an essential function in the progression of CRC. PMID: 24331325
  63. TIE2-mutant endothelial cells are deficient in the production of PDGFB. PMID: 23633549
  64. PDGF-BB regulation of the mesenchymal stem cell differentiation is via Erk signalling PMID: 22805337
  65. Mutations in the gene encoding PDGF-B cause brain calcifications in humans and mice. PMID: 23913003
  66. Overexpression of platelet-derived growth factor-B increases the growth, invasion, and angiogenesis of gastric carcinoma cells through protein kinase B. PMID: 23906294
  67. These data support the view that PDGFB, a mitogenic growth factor, has a limited ability to induce senescence. PMID: 23934686
  68. The present study demonstrated that the PDGF-beta alpha-Syn transgenic mouse model presents with early and progressive accumulation of human alpha-Syn that is accompanied by motor deficits. PMID: 23302418
  69. Our results confirm that analysis of the COL1A1-PDGFB status is a useful tool in the confirmation of a Dermatofibrosarcoma protuberans diagnosis PMID: 23207290
  70. HBXIP promotes the proliferation of breast cancer cells via upregulating PDGFB. PMID: 23537647
  71. Hepatitis B viral (HBV) particles and HBV c and x proteins promote stellate cell proliferation and fibrogenesis in vitro and the PDGF-B/PDGFR-beta signaling pathway is important in this process. PMID: 23042547
  72. Case Report: fusion between COL1A1 exon 31 to exon 2 of PDGFB in atrophic dermatofibrosarcoma protuberans. PMID: 23199263
  73. PDGF-BB has an important role in the development of hypertrophy of ligamentum flavum in lumbar spinal canal stenosis. PMID: 21764712
  74. PDGF-B may enhance tumor proliferation by modulating the expression of oncomiRs and tumor suppressor miRNA-21 in U87 human GBM cells. PMID: 22922228
  75. this study provides a molecular insight into signal amplification of the proteolytic network and PDGF signaling loop during cancer progression.PDGF D is a more potent inducer of prostate epithelial cell migration and invasion than PDGF B PMID: 22689130
  76. Elevated serum levels of PDGF-BB can reflect degree of liver damage and degree of liver fibrosis in chronic hepatitis B. PMID: 22688015
  77. Neutralization of PDGF-B inhibited both the proliferation and the activation of hepatic stellate cells in the fibrotic mouse livers. PMID: 22711911
  78. antiapoptotic gene Bcl-2 enhances tumor formation in a PDGFB-dependent model system of oligodendroglioma. PMID: 21171016
  79. Transcriptionally active beta-catenin regulates expression of PDGF-B in endothelial cells PMID: 22908324
  80. Suggest that phosphorylation of Coro1B and the subsequent reduced interaction with ARP2/3 complex participate in PDGF-induced VSMC migration, an important step in vascular lesion formation. PMID: 22619279
  81. PDGF-BB exhibits cooperative effects with synthetic peptides in differentiation and proliferation. PMID: 22104124
  82. Data show that Only three of these proteins EGF, PDGF-BB and MIP-1delta (CCL15) differed significantly in plasma between controls and Alzheimer's disease (AD). PMID: 22279551
  83. The anti-proliferative effect of EORP(extract of Reishi polysaccharides) was partly mediated by downregulation of PDGF-induced JNK phosphorylation PMID: 22021110
  84. Co-culturing cholangiocarcinoma (CCA)cells with PDGF-BB-secreting myofibroblasts significantly decreased TRAIL-induced CCA cell apoptosis when compared with monoculture conditions. PMID: 22133064
  85. UVC radiation inhibits PDGF-BB-induced migration by suppressing the Akt-GSK3beta pathway in pancreatic cancer cells. PMID: 22200892
  86. sPDGF-BB was significantly higher in esophageal cancer patients than controls and corresponded with the disease advancement. PMID: 21956466
  87. PDGF-BB is not superior to laminin as a potential marker of advanced chronic pancreatitis. PMID: 21921666
  88. myofibroblast-derived PDGF-BB protects cholangiocarcinomacells from TRAIL cytotoxicity by a Hh-signaling-dependent process PMID: 22038837
  89. PDGF both contributes to the osteogenic lineage and helps to stabilize newly forming vessels that act to drive the multistep, multicomponent cascade of new bone formation. PMID: 21618276
  90. PDGFBB and HGF are related to severity of acute pancreatitis, and together with Ang-2 are related to clinical evolution. PMID: 21750433
  91. Suggest the possible involvement of Hif-1alpha/PDGF-BB pathway in the development of HIV-1 associated pulmonary arterial hypertension. PMID: 21819559
  92. During progression of hPDGFb-induced murine gliomas, tumor suppressor loss can expand the recruited cell population not derived from the cell-of-origin within glioma microenvironment. PMID: 21754979
  93. High PDGFB is associated with colorectal cancer. PMID: 21766497
  94. Mesenchymal stem cell migration is regulated by fibronectin through alpha5beta1-integrin-mediated activation of PDGFR-beta and potentiation of PDGF-BB signals. PMID: 21429937
  95. Data show differential expression of interferon-gamma, TNFSF3, TNFSF10, TNFSF12 and PDGFbeta in CD8+, CD14+ and CD4+ cells. PMID: 21211989
  96. The gene that encodes for PDGFB gene is localized on chromosome 22q12.3-q13.1, regions that have reported linkage to schizophrenia. Read More: PMID: 20950212
  97. Periosteal coverage of periodontal defects is not associated with a significant increase in platelet-derived growth factor-BB levels in gingival crevicular fluid. PMID: 20681813
  98. PDGF-BB induces cell proliferation and proinflammatory cytokine expression in rat and human astrocyte cell lines. PMID: 21368226
  99. PDGF-BB and ET-1 synergistically accelerate vascular smooth muscle hyperplastic changes and lose its contractility, at least partially through ERK1/2, Akt, and mTOR activation. PMID: 21129745
  100. Data indicate that PDGF/PDGFRbeta signaling-axis as a critical element of EPC-induced endothelial angiogenesis. PMID: 21124835

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Involvement in disease Basal ganglia calcification, idiopathic, 5 (IBGC5)
Subcellular Location Secreted
Protein Families PDGF/VEGF growth factor family
Tissue Specificity Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
Database Links

HGNC: 8800

OMIM: 190040

KEGG: hsa:5155

STRING: 9606.ENSP00000330382

UniGene: Hs.1976

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