Recombinant Human Interleukin-19 protein (IL19) is produced in E. coli, covering the expression region 25-177aa. This tag-free protein shows purity exceeding 95%, as verified by SDS-PAGE analysis. Endotoxin levels remain below 1.0 EU/μg as determined by the LAL method. The protein maintains full biological activity, with an ED50 of less than 1.5 ng/ml, determined through a cell proliferation assay—suggesting robust activity.
Interleukin-19 (IL19) belongs to the IL-10 cytokine family and appears to play a key role in immune response modulation. It works by binding to receptor complexes that include IL-20Rα and IL-20Rβ, which may influence the signaling pathways that regulate inflammatory responses. This positions IL19 as a significant protein for research aimed at understanding cytokine-mediated communication within the immune system.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. IL-20 Receptor Signaling Pathway Studies
This biologically active recombinant IL-19 protein can investigate the IL-20 receptor signaling cascade in vitro, given its demonstrated activity through IL-20Rα and IL-20Rβ receptors. Researchers might study downstream signaling events, including STAT phosphorylation patterns and gene expression changes in receptor-expressing cell lines. The defined ED50 value provides a quantitative reference for dose-response experiments examining receptor binding kinetics and signal transduction mechanisms.
2. Cell Proliferation and Viability Assays
The protein's validated biological activity in BaF3 cell proliferation assays makes it suitable for investigating IL-19's effects on various cell types expressing IL-20 receptors. Scientists can examine proliferative responses in primary cells or established cell lines, particularly those relevant to immune system function. The high specific activity (>6.7×10⁵ IU/mg) appears to ensure reliable and reproducible results in proliferation-based screening assays, though individual cell line responses may vary.
3. Antibody Development and Validation
This tag-free, high-purity recombinant IL-19 protein serves as an ideal antigen for generating and characterizing anti-IL-19 antibodies for research applications. The protein works well in immunization protocols, ELISA development, and antibody specificity testing. Its preserved biological activity allows for functional antibody screening, potentially helping researchers identify antibodies that block or enhance IL-19 biological function.
4. Protein-Protein Interaction Studies
The biologically active IL-19 protein can be used in binding assays to characterize its interactions with IL-20 receptor subunits and other potential binding partners. Surface plasmon resonance, bio-layer interferometry, or co-immunoprecipitation experiments may help determine binding affinities and kinetic parameters. The low endotoxin level suggests that observed interactions are likely specific to IL-19 rather than contaminating bacterial components.
5. Cytokine Network Analysis
This recombinant IL-19 protein allows researchers to study its role within broader cytokine signaling networks, particularly in the context of IL-20 subfamily interactions. The protein works in multiplex cytokine assays or co-culture experiments to examine how IL-19 influences the production or activity of other cytokines. Such studies might provide insights into the regulatory mechanisms governing immune responses and inflammatory processes in controlled experimental systems, though the complexity of these networks means results may require careful interpretation.
