Code | CSB-MA007670A1m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:5000-1:640000 |
IF | 1:50-1:300 |
FC | 1:100-1:600 |
IP | 2μl-4μl |
In the production of the ENO1 monoclonal antibody, the mouse was injected with recombinant human ENO1 protein (2-434aa) to stimulate its immune system. Following this, B lymphocytes were harvested from the mouse spleen and fused with an immortal myeloma cell line to create hybridomas. The hybridomas were then screened to find those that secreted antibodies with the desired specificity for ENO1. The selected hybridomas were injected into the abdomen of the mouse to generate ascites, which contained the ENO1 monoclonal antibody. This ENO1 monoclonal antibody has been purified with protein G and validated in ELISA, WB, IF, FC, and IP. Its purity is over 95%.
The ENO1 protein is involved in glycolysis by catalyzing the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its metabolic function, ENO1 also plays non-metabolic roles in various cellular processes such as gene transcription, DNA replication, cell migration, and apoptosis. Additionally, ENO1 has been implicated in several disease states, including cancer, autoimmunity, and infectious diseases.
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