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Western Blot
Positive WB detected in: HeLa whole cell lysate(30µg), U251 whole cell lysate(30µg), A431 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), K562 whole cell lysate(30µg), A549 whole cell lysate(30µg), Raji whole cell lysate(30µg)
All lanes: ENO1 antibody at 1:1000
Secondary
Goat polyclonal to Human IgG at 1/40000 dilution
Predicted band size: 47/37 kDa
Observed band size:47 kDa
Exposure time:15s
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IHC image of CSB-RA007670MA3HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA007670MA3HU diluted at 1:50 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA007670MA3HU diluted at 1:50 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Immunofluorescence staining of HepG2 cell with CSB-RA007670MA3HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of Hela cell with CSB-RA007670MA3HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated Goat Anti-Mouse IgG(H+L).
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Overlay Peak curve showing Hela cells stained with CSB-RA007670MA3HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was Mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
