HA-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000141M0m
See More Details Free Antibody trial simple
Size US$200
Image
  • Western Blot
    Positive WB detected in: 3 different overexpression lysates with HA tagged
    All lanes: HA-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35, 35, 48 kDa
    Observed band size: 35, 35, 48 kDa

  • Western Blot
    Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
    All lanes: HA-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Western Blot
    Positive WB detected in: HA-tagged fusion protein
    All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Western Blot
    Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
    All lanes: HA-Tag antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution

  • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

  • Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Product Details

Target Names HA-Tag
Alternative Names HA-Tag
Raised in Mouse
Species Reactivity All
Immunogen YPYDVPDYA synthetic peptide conjugate to KLH
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2b
Clone No. 18B11H6
Purification Method >95%, Protein A purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IF, IP, FC
Recommended Dilution
Application Recommended Dilution
WB 1:5000-1:160000
IF 1:50-1:200
IP 1μg-5μg
FC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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