HA-Tag Monoclonal Antibody

Datasheet
Code CSB-MA000141M0m
Product Type Monoclonal Antibody
Size
Research Use The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Image
  • Western Blot
    Positive WB detected in: 3 different overexpression lysates with HA tagged
    All lanes: HA-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35, 35, 48 kDa
    Observed band size: 35, 35, 48 kDa

  • Western Blot
    Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
    All lanes: HA-Tag antibody at 1:1000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Western Blot
    Positive WB detected in: HA-tagged fusion protein
    All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 35 kDa
    Observed band size: 35 kDa

  • Western Blot
    Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
    All lanes: HA-Tag antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution

  • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

  • Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
    Lane 3: 293F transfected whole cell lysate (20µg)

  • Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Flow Cytometry (FC) Protocol
Immunogen YPYDVPDYA synthetic peptide conjugate to KLH
Raised in Mouse
Species Reactivity All
Specificity Non-conjugated
Tested Applications ELISA, WB, IF, IP, FC; Recommended dilution: WB: 1:5000-1:160000, IF: 1:50-1:200, IP: 1µg-5µg, FC: 1:50-1:200
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein A purified
Isotype IgG2b
Clonality Monoclonal
Alias HA-Tag
Target Names HA-Tag
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Clone No. 18B11H6
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