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Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: PRDX3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 KDa Exposure time:5min
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Immunofluorescence staining of Hela cells with(CSB-MA018656A1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Immunofluorescence staining of MCF7 cells with(CSB-MA018656A1m)at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
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Overlay Peak curve showing Hela cells stained with CSB-MA018656A1m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Overlay Peak curve showing MCF7 cells stained with CSB-MA018656A1m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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