ACE2

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S (CSB-MP3324GMY) at 2 μg/ml can bind human ACE2, the EC₅₀ of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 5 μg/ml can bind human ACE2, the EC₅₀ of human ACE2 protein is 31.80 - 44.69 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind human ACE2, the EC₅₀ is 2.785-9.139 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 μg/ml can bind SARS-CoV-2-S1-RBD (CSB-MP3324GMY1), the EC₅₀ of human ACE2 protein is 8.363-12.82 ng/ml.

SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.

SARS-CoV-2 Spike protein RBD his/myc tag (CSB-MP3324GMY1b1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 13.8 nM as detected by LSPR Assay.

IHC image of CSB-RA866317MA1HU diluted at 1：50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA866317MA1HU diluted at 1：50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA866317LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA866317LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA866317LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)