What is ACE2?
Angiotensin-converting enzyme 2 (ACE2), also known as ACAH, is a protein in humans that is encoded by ACE2 gene. It was identified as the first reported ACE homologue in 2000. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases. Both ACE2 and ACE belong to the renin-angiotensin-aldosterone system (RAAS) and kallikrein-bradykinin system (KKS) families. ACE2 is a metalloproteinase with a total length of 805 amino acids. It is a type I transmembrane glycoprotein with a single extracellular catalytic domain. The human ACE2 gene has been cloned and mapped to the X chromosome.
What is the feature of ACE2 structure?
Like ACE, ACE2 has two domains: an amino-terminal catalytic domain and a carboxy-terminal domain. The catalytic domain has an active site called the zinc metallopeptidase domain (HEXH motif) and shows 41.8% sequence identity with the amino domain of ACE. The carboxy-terminal domain of ACE2 has 48% sequence identity with Collectrin. Collectrin is a non-catalytic protein that has key roles in renal amino acid reabsorption, pancreatic β-cell proliferation, and possible insulin exocytosis.
What is the function of ACE2?
Earlier studies revealed that ACE2 is mainly localized in the heart, kidney, and testis, and is expressed at low levels in many other tissues, especially the colon and lung. Later studies have also shown that ACE2 also plays an important role in other organs such as the liver and intestine. ACE2 is an important regulatory molecule of the renin-angiotensin system (RAS), which plays an important role in normal physiological activities to control cardiovascular and blood pressure. In addition, ACE2 is a receptor for SARS and other related viruses, and plays a key role in virus invasion.
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Popular Product:
● Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active) CSB-MP866317HU
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 5 μg/ml can bind human ACE2, the EC50 of human ACE2 protein is 31.80 - 44.69 ng/ml.
LSPR
SARS-CoV-2 Spike protein RBD his/sumostar tag (CSB-YP3324GMY1) captured on COOH chip can bind Human ACE2 protein Fc tag (CSB-MP866317HU) with an affinity constant of 100 nM as detected by LSPR Assay.
● Recombinant Macaca fascicularis Angiotensin-converting enzyme(ACE2),partial (Active) CSB-MP3414MOV
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD (CSB-MP3324GMY1b1) at 5 μg/ml can bind Cynomolgus-ACE2, the EC50 is 5.638-9.496 ng/ml.
● Recombinant Paguma larvata Angiotensin-converting enzyme 2(ACE2),partial (Active) CSB-MP684964PAL
Validated Data
SDS
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA
Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 μg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.
HPLP
The purity of ACE2 was greater than 90% as determined by SEC-HPLC
