Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

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Name: Human Angiotensin converting enzyme 2, ACE2 ELISA Kit
Brand: CUSABIO
SKU: CSB-E04489h

Product Details

Description

CUSABIO's product CSB-E04489h is a ready-to-use ELISA kit designed for the quantitative measurement of ACE2 concentrations in serum, plasma, or cell culture supernates. It provides a detection range of 0.156 ng/mL-10 ng/mL. It has high sensitivity as low as 0.039 ng/mL. Validated parameters of this kit also include precision, linearity, and recovery.

This kit contains all reagents necessary for quantitatively detecting ACE2 levels. In this assay, an anti-human ACE2 antibody has been pre-coated on the microplate. Samples and standards are respectively added to wells. After thorough washing, a biotin-conjugated ACE2 antibody is added to wells. ACE2 in the samples or in the standards is sandwiched between the twp antibody pairs. Following a rigorous washing, HRP-avidin conjugate is pipetted into wells. The solution turns blue after the addition of the TMB substrate. The color reaction is terminated when adding the stop solution, and the solution turns blue to yellow. The color intensity is positively correlated to ACE2 concentrations in the sample and is measured by reading the absorption value at 450 nm in a microplate reader.

ACE2, on the one hand, catalyzes the conversion of Ang I and Ang II into the inactive Ang 1-9 and Ang 1-7 as a carboxypeptidase in the renin-angiotensin-aldosterone system (RAAS). Through the breakout of Ang II into other molecules, ACE2 regulates blood pressure, maintains blood pressure homeostasis, and reduces inflammation and tissue injury. On the other hand, ACE2 has been found to serve as a receptor for SARS-CoV, NL63 coronavirus, and SARS-CoV-2. In the SARS-CoV and SARS-CoV-2 infection, ACE2 on the host cells binds to viral spike (S) protein promoting the viral entry into host cells.

Target Name

angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

Alternative Names

ACE 2 ELISA Kit; ACE related carboxypeptidase ELISA Kit; ACE-related carboxypeptidase ELISA Kit; ACE2 ELISA Kit; ACE2_HUMAN ELISA Kit; ACEH ELISA Kit; Angiotensin converting enzyme 2 ELISA Kit; Angiotensin converting enzyme homolog ELISA Kit; Angiotensin converting enzyme like protein ELISA Kit; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 ELISA Kit; Angiotensin I converting enzyme 2 ELISA Kit; Angiotensin-converting enzyme homolog ELISA Kit; DKFZP434A014 ELISA Kit; EC 3.4.17 ELISA Kit; metalloprotease MPROT 15 ELISA Kit; Metalloprotease MPROT15 ELISA Kit; OTTHUMP00000022963 ELISA Kit; Processed angiotensin-converting enzyme 2 ELISA Kit

Abbreviation

ACE2

Uniprot No.

Q9BYF1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-99
1:2	Average %	97
	Range %	90-105
1:4	Average %	95
	Range %	91-107
1:8	Average %	93
	Range %	80-98

Recovery

The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	97	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.216	2.182	2.199	1.955
5	2.012	1.916	1.964	1.720
2.5	1.754	1.643	1.699	1.455
1.25	1.326	1.290	1.308	1.064
0.625	1.008	0.944	0.976	0.732
0.312	0.714	0.676	0.695	0.451
0.156	0.416	0.392	0.404	0.160
0	0.249	0.239	0.244

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human AE2 antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ACE2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Target Background

Function (From Uniprot)	Angiotensin-converting enzyme 2 (ACE2), also known as ACAH, is an essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system, which is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. ACE2 is a suppressor of renin-angiotensin system (RAS). ACE2 converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, a vasodilator. Besides the role in the cardiovascular system, ACE2 also plays a role in lung diseases as a receptor for SARS-CoV-2. Recently, with the COVID19 caused by SARS-CoV-2 spreads around the world, ACE2 functions as a receptor on the cell surface to bind with spike protein of SARS-CoV-2 in SARS-CoV-2 infection. And the mechanism of SARS-CoV-2 invasion via ACE2 are gradually being revealed. Based on these studies, there are more coronavirus-host interactome targets which have been found, such as TMPRSS2 and CD147. All of these works are critical to SARS-CoV-2 treatment, and provide potential targets of drug development for SARS-CoV-2 therapy.
Gene References into Functions	Report no influence of ACE2 gene polymorphism on stroke recurrence and only find possible interaction between hypertension history and the ACE2 gene in male stroke patients. PMID: 30056001 In a Chinese Han population, five single-nucleotide polymorphisms (SNP) (rs1514283, rs4646155, rs4646176, rs2285666, and rs879922) in ACE2 gene were determined to significantly associate with essential hypertension in female participants, while no SNP locus was linked to male group. PMID: 30335025 ACE2 and other enzymes can form ANG-(1-7) directly or indirectly from either the decapeptide ANG I or from ANG II. [review] PMID: 29351514 Suggest a potential role for ACE2 polymorphism in postexercise hypotension in hypertensive medicated individuals. PMID: 27925380 ACE2 SNPs and haplotypes are associated with circulating angiotensin-(1-7) levels. PMID: 28895159 we found an interaction between ACE2 and AGTR1 in structuralatrial fibrillation patients in a Chinese Han population PMID: 29441892 results suggest that ACE2, TNNI3K and CALM3 polymorphisms are associated with increased risk of hypertrophic cardiomyopathies and dilated cardiomyopathies and may act as disease modifiers of these diseases. PMID: 28744816 Elevated plasma ACE2 is significantly associated with more advanced LA structural remodeling in atrial fibrillation. PMID: 27738071 Overexpression of ACE2 in monocytes led to reduced endothelial adhesion, transmigration and downregulation of adhesion-related molecules and results in atherosclerosis. PMID: 28186543 These results indicated that aberrant methylation of the ACE2 promoter may be associated with EH risk. In addition, sex may significantly influence ACE2 methylation. PMID: 28440441 ACE2 rs2106809 is an important predictive factor of the response to antihypertensive treatment with ACE inhibitors in Chinese female hypertensive patients. PMID: 27121444 In islets from db/db mice, ACE2 over-expression increased intracellular calcium influx and restored impaired mitochondrial oxidation, potentially causing an increase in GSIS. These results shed light on the potential roles of ACE2 in mitochondrial metabolism, moreover, may improve our understanding of diabetes. PMID: 29128354 ACE2 may have a role in silent atherosclerosis in patients with chronic kidney disease; it counterbalances the vasoconstrictor adverse effects of angiotensin II by its conversion PMID: 27615597 This study reveals an elevated serum concentration of ACE2 and independent associations between serum ACE2 and echocardiographic parameters in hypertensive patients. PMID: 28223093 The findings of this study indicate that ACE-2 activity is reduced in AD and is an important regulator of the central classical ACE-1/Ang II/AT1R axis of renin-angiotensin system, and also that dysregulation of this pathway likely plays a significant role in the pathogenesis of Alzheimer's disease. PMID: 27884212 Overexpression of ACE2 ameliorates Abeta-induced inflammatory response by activating the ACE2/Ang-(1-7)/Mas axis in human RPE cells. PMID: 28605813 Although further studies are required to determine how clusterin suppresses non-specific cellular uptake in phagocytes, our data suggest that clusterin plays a key role in the stealth effect of not only pegylated nanoparticles but also non-pegylated nanoparticles. PMID: 27983983 The ACE2 G8790A polymorphism in type 2 diabetes mellitus patients was correlated with cerebral stroke, and the A allele might be a risk factor of type 2 diabetes mellitus combined with cerebral stroke. PMID: 27500554 ollectrin, an ACE2 homolog with no catalytic activity, regulates blood pressure through an NO-dependent mechanism. Large body of experimental data confirmed sustained beneficial effects of ACE2/Ang-(1-7)/Mas receptor axis activation on hypertension and vascular injury. PMID: 27889958 The potential contribution of the ACE2 to cardiovascular disease progression was addressed. PMID: 27965422 Serum ACE2 activity was significantly lower in acute ischemic stroke as compared to both control and stroke-alert patients, followed by an increase to control levels at three days. PMID: 27488276 Here, we review the role and effects of ACE2, ACE2 activators, Ang-(1-7) and synthetic Mas receptor agonists in the control of inflammation and fibrosis in cardiovascular and renal diseases and as counter-regulators of the ACE-Ang II-AT1 axis. PMID: 26995300 ACE2 overexpression inhibited cell growth. PMID: 27460845 Downregulation of ACE2/Ang-(1-7)/Mas axis stimulates breast cancer metastasis through the activation of store-operated calcium entry and PAK1/NF-kappaB/Snail1 pathways. PMID: 27063099 The circulating ACE2 and Ang-(1-7) levels were related to neither rs4646155 nor rs879922 in female or male patients.In conclusion, the rs2106809 polymorphism of the ACE2 gene may be a determinant of the circulating Ang-(1-7) level in female patients with hypertension, suggesting a genetic association between circulating Ang-(1-7) levels and ACE2 gene polymorphisms in patients with hypertension. PMID: 27310975 These results indicated that angiotensin-(1-7)/ACE2/Mas axis may reduce liver lipid accumulation partly by regulating lipid-metabolizing genes through ATP/P2 receptor/CaM signaling pathway. PMID: 26883384 ACE-2 significantly increased when IMR-90 were hypoxic prior to hyperoxic exposure with no recovery. PMID: 27093376 Imbalanced down-regulation of ACE and ACE2 mRNA expression levels may play an important role in the development and progression of thoracic aortic aneurysmal dilatation and subsequently dissection. PMID: 25237166 These results suggest that the ACE2 G8790A and rs2106809 polymorphisms may be associated with essential hypertension risk. PMID: 25237167 ACE and ACE2 expression at the mRNA and protein levels are significantly increased in the myocardium of patients with heart failure. PMID: 25869724 Multivariable regression analysis revealed that urinary L-FABP and urinary albumin/ creatinine ratio were significantly associated with urinary ACE2 levels. PMID: 26067610 ACE2 and Ang-(1-7) significantly inhibit early atherosclerotic lesion formation via protection of endothelial function and inhibition of inflammatory response. PMID: 25721616 ACE-2 is expressed in fetal human lung fibroblasts but is significantly decreased by hyperoxic gas PMID: 25665060 urinary ACE2 increased in type 2 diabetic patients with various degrees of albuminuria PMID: 25791940 soluble ACE2 is involved in the pathomechanism of hypertension and heart failure. PMID: 24691269 Decrease in circulating ACE2 activity was associated with cardiovascular disease in patients with chronic kidney disease. PMID: 25813276 By genetic replenishment of ACE2 and pharmaceutical use of ARB, restored ACE2 level mitigated GBC growth. Our results supported the rationale for the use of ARB in GBC patients for potential therapeutic benefit PMID: 25663464 There is no clear association between ACE2 gene A9570G polymorphisms and childhood primary nephrotic syndrome. PMID: 25815490 Our results revealed that SNPs rs2074192 and rs714205 in ACE2 gene were associated with the susceptibility of DR and PDR. PMID: 25359286 Olmesartan may uniquely increase urinary ACE2 level in hypertensive patients, which could potentially offer additional renoprotective effects. PMID: 24842388 Hepatocellular carcinoma patients with higher level of ACE2 expression had longer survival time than those with lower level of ACE2 expression. PMID: 25701390 Urinary ACE2 activity and protein expression are increased in type 1 diabetes patients prior to the onset of clinical complications. PMID: 24920267 Brain endoplasmic reticulum stress does not contribute to DOCA-salt hypertension and ACE2 blunts neurogenic hypertension independently of ER stress. PMID: 25519733 Partial loss of ACE2 is sufficient to enhance the susceptibility to heart disease. PMID: 24728465 These data demonstrate that MSCs modified to overexpress the ACE2 gene can produce biologically active ACE2 protein over a sustained period of time and have an enhanced ability to promote endothelial repair after LPS challenge PMID: 25200929 ACE2 cleavage is regulated by influenza A H1N1 neuraminidase. PMID: 24662240 among females ACE2 rs2106809 polymorphisms,and among males ACE2 rs2106809 polymorphism and alcohol consumption are associated with essential hypertension PMID: 24112034 Data suggest angiotensin (ANG) converting enzyme 2/ANG-II-(1-7)/proto-oncogene protein Mas axis regulates inflammation/fibrosis, cell proliferation, and leukocyte recruitment/activation; main topic here is kidney/inflammatory renal disease. [REVIEW] PMID: 23488800 ACE2 and FZD1 are prognosis markers in squamous cell/adenosquamous carcinoma and adenocarcinoma of gallbladder. PMID: 23921915 ACE2 overexpression in the rostral ventrolateral medulla attenuates the enhanced tonically active glutamatergic input in SHRs, which may be an important mechanism underlying the beneficial effect of central ACE2 to hypertension. PMID: 24838502 Show More Hide All
Subcellular Location	Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families	Peptidase M2 family
Tissue Specificity	Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidn
Database Links	HGNC: 13557 OMIM: 300335 KEGG: hsa:59272 STRING: 9606.ENSP00000252519 UniGene: Hs.178098

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Check Code：

Code	CSB-E04489h
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.