Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

Code CSB-E04489h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9BYF1
Lead Time 3-5 working days
Abbreviation ACE2
Protein Biological Process 1 Immunity
Target Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Alias ACEH, DKFZp434A014, ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein
Species Homo sapiens (Human)
Protein Biological Process 3 Host-virus interaction
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
HGNC 13557
RGD 728890
MGI 1917258
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %80-99
1:2Average %97
Range %90-105
1:4Average %95
Range %91-107
1:8Average %93
Range %80-98
Recovery
The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9288-98
EDTA plasma (n=4)9790-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.216 2.182 2.199 1.955
52.012 1.916 1.964 1.720
2.51.754 1.643 1.699 1.455
1.251.326 1.290 1.308 1.064
0.6251.008 0.944 0.976 0.732
0.3120.714 0.676 0.695 0.451
0.1560.416 0.392 0.404 0.160
00.249 0.239 0.244  
References
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Function Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidn
Database Links

HGNC: 13557

OMIM: 300335

KEGG: hsa:59272

STRING: 9606.ENSP00000252519

UniGene: Hs.178098

Pathway Renin-angiotensin system

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