Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

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Target Data

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Product Details

Target Name

angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

Alternative Names

ACEH, DKFZp434A014, ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein

Abbreviation

ACE2

Uniprot No.

Q9BYF1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-99
1:2	Average %	97
	Range %	90-105
1:4	Average %	95
	Range %	91-107
1:8	Average %	93
	Range %	80-98

Recovery

The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	97	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.216	2.182	2.199	1.955
5	2.012	1.916	1.964	1.720
2.5	1.754	1.643	1.699	1.455
1.25	1.326	1.290	1.308	1.064
0.625	1.008	0.944	0.976	0.732
0.312	0.714	0.676	0.695	0.451
0.156	0.416	0.392	0.404	0.160
0	0.249	0.239	0.244

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

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Target Data

Function	Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location	Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families	Peptidase M2 family
Tissue Specificity	Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidn
Database Links	HGNC: 13557 OMIM: 300335 KEGG: hsa:59272 STRING: 9606.ENSP00000252519 UniGene: Hs.178098

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Check Code：

Code	CSB-E04489h
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.