CUSABIO's product CSB-E04489h is a ready-to-use ELISA kit designed for the quantitative measurement of ACE2 concentrations in serum, plasma, or cell culture supernates. It provides a detection range of 0.156 ng/mL-10 ng/mL. It has high sensitivity as low as 0.039 ng/mL. Validated parameters of this kit also include precision, linearity, and recovery.
This kit contains all reagents necessary for quantitatively detecting ACE2 levels. In this assay, an anti-human ACE2 antibody has been pre-coated on the microplate. Samples and standards are respectively added to wells. After thorough washing, a biotin-conjugated ACE2 antibody is added to wells. ACE2 in the samples or in the standards is sandwiched between the twp antibody pairs. Following a rigorous washing, HRP-avidin conjugate is pipetted into wells. The solution turns blue after the addition of the TMB substrate. The color reaction is terminated when adding the stop solution, and the solution turns blue to yellow. The color intensity is positively correlated to ACE2 concentrations in the sample and is measured by reading the absorption value at 450 nm in a microplate reader.
ACE2, on the one hand, catalyzes the conversion of Ang I and Ang II into the inactive Ang 1-9 and Ang 1-7 as a carboxypeptidase in the renin-angiotensin-aldosterone system (RAAS). Through the breakout of Ang II into other molecules, ACE2 regulates blood pressure, maintains blood pressure homeostasis, and reduces inflammation and tissue injury. On the other hand, ACE2 has been found to serve as a receptor for SARS-CoV, NL63 coronavirus, and SARS-CoV-2. In the SARS-CoV and SARS-CoV-2 infection, ACE2 on the host cells binds to viral spike (S) protein promoting the viral entry into host cells.