Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

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Target

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ACE2 Antibodies

ACE2 Proteins

ACE2 Antibodies

ACE2 Proteins

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Human Angiotensin converting enzyme 2, ACE2 ELISA Kit

Specification
Target

Code

CSB-E04489h

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

Q9BYF1

Lead Time

3-5 working days

Abbreviation

ACE2

Protein Biological Process 1

Immunity

Target Name

angiotensin I converting enzyme (peptidyl-dipeptidase A) 2

Alias

ACEH, DKFZp434A014, ACE-related carboxypeptidase|angiotensin I converting enzyme 2|angiotensin converting enzyme-like protein

Species

Homo sapiens (Human)

Protein Biological Process 3

Host-virus interaction

Sample Types

serum, plasma, cell culture supernates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

Target Details

This protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

HGNC

13557

RGD

728890

MGI

1917258

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-99
1:2	Average %	97
	Range %	90-105
1:4	Average %	95
	Range %	91-107
1:8	Average %	93
	Range %	80-98

Recovery

The recovery of human ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	97	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.216	2.182	2.199	1.955
5	2.012	1.916	1.964	1.720
2.5	1.754	1.643	1.699	1.455
1.25	1.326	1.290	1.308	1.064
0.625	1.008	0.944	0.976	0.732
0.312	0.714	0.676	0.695	0.451
0.156	0.416	0.392	0.404	0.160
0	0.249	0.239	0.244

References

Evaluation of Serum Levels of Renin Angiotensin System Components in Asthmatic Patients Ceylan Ayada1. et al,Erciyes Med J ,37(3): 87-90
N-Acetyl-Seryl-Asparyl-Lysyl-Proline interaction with ACE2-Ang (1-7)-Mas axis attenuates pulmonary fibrosis in silicotic rats Xuemin G, et al,Experimental Physiology,July 9

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Function	Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function.
Subcellular Location	Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families	Peptidase M2 family
Tissue Specificity	Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidn
Database Links	HGNC: 13557 OMIM: 300335 KEGG: hsa:59272 STRING: 9606.ENSP00000252519 UniGene: Hs.178098
Pathway	Renin-angiotensin system

ACE2 Antibody

ACE2 Protein

ACE2 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA001150GA01HU	ACE2 Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA040178	ACE2 Antibody	Human,Mouse	WB,ELISA
CSB-PA111496	ACE2 Antibody	Human	ELISA,IHC
CSB-PA025395	ACE2 Antibody	Human,Mouse,Rat	ELISA,IHC
CSB-PA445794	ACE2 Antibody	Human,Mouse,Rat	ELISA,IHC

ACE2 Antibodies for Human

Code	Product Name	Species Reactivity	Application
CSB-PA866317LA01HU	ACE2 Antibody	Human	ELISA, IHC, IF
CSB-PA866317LB01HU	ACE2 Antibody, HRP conjugated	Human	ELISA
CSB-PA866317LC01HU	ACE2 Antibody, FITC conjugated	Human	ELISA
CSB-PA866317LD01HU	ACE2 Antibody, Biotin conjugated	Human	ELISA

ACE2 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-AP005671HU	Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial (Active)	Mammalian cell
CSB-YP866317HU CSB-EP866317HU CSB-BP866317HU CSB-MP866317HU	Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.