Recombinant Human Angiotensin-converting enzyme 2(ACE2),partial(Active)

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Code CSB-AP005671HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Immobilized Human ACE-2 at 10μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-mFc .The ED50 of Recombinant 2019-nCoV S Protein RBD-mFc is 62 ng/ml .
Target Names ACE2
Uniprot No. Q9BYF1
Research Area Cardiovascular
Alternative Names ACE 2; ACE related carboxypeptidase; ACE-related carboxypeptidase; ACE2; ACE2_HUMAN; ACEH; Angiotensin converting enzyme 2; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2; Angiotensin I converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZP434A014; EC 3.4.17; metalloprotease MPROT 15; Metalloprotease MPROT15; OTTHUMP00000022963; Processed angiotensin-converting enzyme 2
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 18-740aa
Complete Sequence QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
Mol. Weight 84.63 kDa
Protein Length Partial
Tag Info C-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Buffer 0.2 μm Filtered 20 mM Tris-HCl, 300 mM NaCl, 1 mM ZnCl2, 10% Glycerol, pH 7.4
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Datasheet & COA Please contact us to get it.

Target Data

Function Angiotensin-converting enzyme 2 (ACE2), also known as ACAH, is an essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system, which is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. ACE2 is a suppressor of renin-angiotensin system (RAS). ACE2 converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, a vasodilator. Besides the role in the cardiovascular system, ACE2 also plays a role in lung diseases as a receptor for SARS-CoV-2. Recently, with the COVID19 caused by SARS-CoV-2 spreads around the world, ACE2 functions as a receptor on the cell surface to bind with spike protein of SARS-CoV-2 in SARS-CoV-2 infection. And the mechanism of SARS-CoV-2 invasion via ACE2 are gradually being revealed. Based on these studies, there are more coronavirus-host interactome targets which have been found, such as TMPRSS2 and CD147. All of these works are critical to SARS-CoV-2 treatment, and provide potential targets of drug development for SARS-CoV-2 therapy.
Gene References into Functions
  1. Report no influence of ACE2 gene polymorphism on stroke recurrence and only find possible interaction between hypertension history and the ACE2 gene in male stroke patients. PMID: 30056001
  2. In a Chinese Han population, five single-nucleotide polymorphisms (SNP) (rs1514283, rs4646155, rs4646176, rs2285666, and rs879922) in ACE2 gene were determined to significantly associate with essential hypertension in female participants, while no SNP locus was linked to male group. PMID: 30335025
  3. ACE2 and other enzymes can form ANG-(1-7) directly or indirectly from either the decapeptide ANG I or from ANG II. [review] PMID: 29351514
  4. Suggest a potential role for ACE2 polymorphism in postexercise hypotension in hypertensive medicated individuals. PMID: 27925380
  5. ACE2 SNPs and haplotypes are associated with circulating angiotensin-(1-7) levels. PMID: 28895159
  6. we found an interaction between ACE2 and AGTR1 in structuralatrial fibrillation patients in a Chinese Han population PMID: 29441892
  7. results suggest that ACE2, TNNI3K and CALM3 polymorphisms are associated with increased risk of hypertrophic cardiomyopathies and dilated cardiomyopathies and may act as disease modifiers of these diseases. PMID: 28744816
  8. Elevated plasma ACE2 is significantly associated with more advanced LA structural remodeling in atrial fibrillation. PMID: 27738071
  9. Overexpression of ACE2 in monocytes led to reduced endothelial adhesion, transmigration and downregulation of adhesion-related molecules and results in atherosclerosis. PMID: 28186543
  10. These results indicated that aberrant methylation of the ACE2 promoter may be associated with EH risk. In addition, sex may significantly influence ACE2 methylation. PMID: 28440441
  11. ACE2 rs2106809 is an important predictive factor of the response to antihypertensive treatment with ACE inhibitors in Chinese female hypertensive patients. PMID: 27121444
  12. In islets from db/db mice, ACE2 over-expression increased intracellular calcium influx and restored impaired mitochondrial oxidation, potentially causing an increase in GSIS. These results shed light on the potential roles of ACE2 in mitochondrial metabolism, moreover, may improve our understanding of diabetes. PMID: 29128354
  13. ACE2 may have a role in silent atherosclerosis in patients with chronic kidney disease; it counterbalances the vasoconstrictor adverse effects of angiotensin II by its conversion PMID: 27615597
  14. This study reveals an elevated serum concentration of ACE2 and independent associations between serum ACE2 and echocardiographic parameters in hypertensive patients. PMID: 28223093
  15. The findings of this study indicate that ACE-2 activity is reduced in AD and is an important regulator of the central classical ACE-1/Ang II/AT1R axis of renin-angiotensin system, and also that dysregulation of this pathway likely plays a significant role in the pathogenesis of Alzheimer's disease. PMID: 27884212
  16. Overexpression of ACE2 ameliorates Abeta-induced inflammatory response by activating the ACE2/Ang-(1-7)/Mas axis in human RPE cells. PMID: 28605813
  17. Although further studies are required to determine how clusterin suppresses non-specific cellular uptake in phagocytes, our data suggest that clusterin plays a key role in the stealth effect of not only pegylated nanoparticles but also non-pegylated nanoparticles. PMID: 27983983
  18. The ACE2 G8790A polymorphism in type 2 diabetes mellitus patients was correlated with cerebral stroke, and the A allele might be a risk factor of type 2 diabetes mellitus combined with cerebral stroke. PMID: 27500554
  19. ollectrin, an ACE2 homolog with no catalytic activity, regulates blood pressure through an NO-dependent mechanism. Large body of experimental data confirmed sustained beneficial effects of ACE2/Ang-(1-7)/Mas receptor axis activation on hypertension and vascular injury. PMID: 27889958
  20. The potential contribution of the ACE2 to cardiovascular disease progression was addressed. PMID: 27965422
  21. Serum ACE2 activity was significantly lower in acute ischemic stroke as compared to both control and stroke-alert patients, followed by an increase to control levels at three days. PMID: 27488276
  22. Here, we review the role and effects of ACE2, ACE2 activators, Ang-(1-7) and synthetic Mas receptor agonists in the control of inflammation and fibrosis in cardiovascular and renal diseases and as counter-regulators of the ACE-Ang II-AT1 axis. PMID: 26995300
  23. ACE2 overexpression inhibited cell growth. PMID: 27460845
  24. Downregulation of ACE2/Ang-(1-7)/Mas axis stimulates breast cancer metastasis through the activation of store-operated calcium entry and PAK1/NF-kappaB/Snail1 pathways. PMID: 27063099
  25. The circulating ACE2 and Ang-(1-7) levels were related to neither rs4646155 nor rs879922 in female or male patients.In conclusion, the rs2106809 polymorphism of the ACE2 gene may be a determinant of the circulating Ang-(1-7) level in female patients with hypertension, suggesting a genetic association between circulating Ang-(1-7) levels and ACE2 gene polymorphisms in patients with hypertension. PMID: 27310975
  26. These results indicated that angiotensin-(1-7)/ACE2/Mas axis may reduce liver lipid accumulation partly by regulating lipid-metabolizing genes through ATP/P2 receptor/CaM signaling pathway. PMID: 26883384
  27. ACE-2 significantly increased when IMR-90 were hypoxic prior to hyperoxic exposure with no recovery. PMID: 27093376
  28. Imbalanced down-regulation of ACE and ACE2 mRNA expression levels may play an important role in the development and progression of thoracic aortic aneurysmal dilatation and subsequently dissection. PMID: 25237166
  29. These results suggest that the ACE2 G8790A and rs2106809 polymorphisms may be associated with essential hypertension risk. PMID: 25237167
  30. ACE and ACE2 expression at the mRNA and protein levels are significantly increased in the myocardium of patients with heart failure. PMID: 25869724
  31. Multivariable regression analysis revealed that urinary L-FABP and urinary albumin/ creatinine ratio were significantly associated with urinary ACE2 levels. PMID: 26067610
  32. ACE2 and Ang-(1-7) significantly inhibit early atherosclerotic lesion formation via protection of endothelial function and inhibition of inflammatory response. PMID: 25721616
  33. ACE-2 is expressed in fetal human lung fibroblasts but is significantly decreased by hyperoxic gas PMID: 25665060
  34. urinary ACE2 increased in type 2 diabetic patients with various degrees of albuminuria PMID: 25791940
  35. soluble ACE2 is involved in the pathomechanism of hypertension and heart failure. PMID: 24691269
  36. Decrease in circulating ACE2 activity was associated with cardiovascular disease in patients with chronic kidney disease. PMID: 25813276
  37. By genetic replenishment of ACE2 and pharmaceutical use of ARB, restored ACE2 level mitigated GBC growth. Our results supported the rationale for the use of ARB in GBC patients for potential therapeutic benefit PMID: 25663464
  38. There is no clear association between ACE2 gene A9570G polymorphisms and childhood primary nephrotic syndrome. PMID: 25815490
  39. Our results revealed that SNPs rs2074192 and rs714205 in ACE2 gene were associated with the susceptibility of DR and PDR. PMID: 25359286
  40. Olmesartan may uniquely increase urinary ACE2 level in hypertensive patients, which could potentially offer additional renoprotective effects. PMID: 24842388
  41. Hepatocellular carcinoma patients with higher level of ACE2 expression had longer survival time than those with lower level of ACE2 expression. PMID: 25701390
  42. Urinary ACE2 activity and protein expression are increased in type 1 diabetes patients prior to the onset of clinical complications. PMID: 24920267
  43. Brain endoplasmic reticulum stress does not contribute to DOCA-salt hypertension and ACE2 blunts neurogenic hypertension independently of ER stress. PMID: 25519733
  44. Partial loss of ACE2 is sufficient to enhance the susceptibility to heart disease. PMID: 24728465
  45. These data demonstrate that MSCs modified to overexpress the ACE2 gene can produce biologically active ACE2 protein over a sustained period of time and have an enhanced ability to promote endothelial repair after LPS challenge PMID: 25200929
  46. ACE2 cleavage is regulated by influenza A H1N1 neuraminidase. PMID: 24662240
  47. among females ACE2 rs2106809 polymorphisms,and among males ACE2 rs2106809 polymorphism and alcohol consumption are associated with essential hypertension PMID: 24112034
  48. Data suggest angiotensin (ANG) converting enzyme 2/ANG-II-(1-7)/proto-oncogene protein Mas axis regulates inflammation/fibrosis, cell proliferation, and leukocyte recruitment/activation; main topic here is kidney/inflammatory renal disease. [REVIEW] PMID: 23488800
  49. ACE2 and FZD1 are prognosis markers in squamous cell/adenosquamous carcinoma and adenocarcinoma of gallbladder. PMID: 23921915
  50. ACE2 overexpression in the rostral ventrolateral medulla attenuates the enhanced tonically active glutamatergic input in SHRs, which may be an important mechanism underlying the beneficial effect of central ACE2 to hypertension. PMID: 24838502
  51. Serum ACE2 level was significantly increased in acute MI patients compared with that of non-MI controls. PMID: 24134599
  52. ACE2 expression in glomeruli is associated with mesangial hypercellularity in early lesions of pediatric IgA nephropathy PMID: 24158104
  53. The results indicate that polymorphism at ACE2 gene is associated with male lone AF in a Chinese Han population. PMID: 24342297
  54. Up-regulation of the ACE2/Ang-(1-7)/Mas axis protected against pulmonary fibrosis by inhibiting the MAPK/NF-kappaB pathway. PMID: 24168260
  55. ACE2 may be subject to post-transcriptional regulation and reveals a novel potential therapeutic target, miR-421, which could be exploited to modulate ACE2 expression in disease PMID: 24564768
  56. In patients admitted with acute decompensated heart failure, increasing serum ACE2 activity levels during intensive medical therapy predict improved outcomes. PMID: 24054336
  57. The objective of this research was to determine whether invasively measured central pulse pressure (PP) in patients indicated for coronarography is associated with two common polymorphisms in the ACE2 region. PMID: 23077079
  58. Genetic polymorphism of G8790A in ACE2 gene plays an important role on the pathogenesis of male patients with type 2 diabetes mellitus and coronary heart disease. PMID: 24142614
  59. A cellular protective role of ACE2 in Ang II-mediated oxidative stress and vascular smooth muscle cell proliferation. PMID: 23816468
  60. TMPRSS2 competing with the ADAM17 for ACE2 processing, but only cleavage by TMPRSS2 resulted in augmented SARS-S-driven entry. PMID: 24227843
  61. Angiotensin converting enzyme 2 in type 2 diabetes mellitus and the therapeutic possibilities of regulating the renin-angiotensin system. [Review] PMID: 23893738
  62. established that the expression of the ACE2 transcript is controlled by the activity of SIRT1 under conditions of energy stress PMID: 24147777
  63. Several ACE2 gene SNPs are associated with blood pressure or blood pressure change in persons of European descent. PMID: 24191856
  64. conclude that HNF1alpha can induce the expression of ACE2 in pancreatic islet cells via evolutionarily conserved HNF1 binding sites in the ACE2 promoter PMID: 24100303
  65. a bat SARS-like coronavirus can use the human ACE2 receptor for viral entry into human cells PMID: 24172901
  66. ADAM17-mediated shedding impairs brain ACE2 compensatory activity, thus contributing to the development of neurogenic hypertension. PMID: 24014829
  67. Serum sACE2 activity rises in relation to infarct size, left ventricular systolic dysfunction and is associated with the occurrence of left ventricular remodeling following myocardial infarction. PMID: 23630610
  68. ACE2 expression attenuates the metastasis of lung cancer in a mouse model. PMID: 23545945
  69. This review summarizes and discusses the structure and multiple functions of ACE2 and the relevance of this key enzyme in disease pathogenesis. PMID: 23328447
  70. The patients with essential hypertension carrying the A/AA allele of the ACE2 gene have a higher risk of stroke than patients with a different allele. PMID: 20813695
  71. Treatment with rhACE2 inhibited Ang II-mediated phosphorylation of the myocardial extracellular signal-regulated kinase 1/2 pathway in WKY rats, with congruent results seen in spontaeously hypertensive rat hearts. PMID: 22750422
  72. Expression of ACE2 and its end product Ang1-7 is increased in diabetic atheroma, along with overexpression of AT1R, IL6, TNF-alpha, macrophage infiltration, and neovascularization. PMID: 22749485
  73. This study showed that ACE2/Ang-(1-7)\Mas pathway activation corrects existing diabetes-induced CD34(+) cell dysfunction and also confers protection from development of this dysfunction.[Mas receptor] PMID: 23230080
  74. podocyte-specific overexpression of ACE2 transiently attenuates the development of diabetic nephropathy. PMID: 22475818
  75. The polymorphisms in the AT1R (rs5186) and ACE2 (rs2285666) genes seem to play lesser roles in the development of type 2 diabetes compared to ACE1. PMID: 21670585
  76. Our data suggest that the urinary ACE2 level is closely associated with type 2 diabetes mellitus and is an independent risk factor for microalbuminuria. PMID: 23144053
  77. The ACE/ACE2 ratio combined with MMP-9 activity in pleural fluid may be potential biomarkers for diagnosing tuberculous pleurisy. PMID: 23091417
  78. urinary ACE2 is increased in renal transplant recipients with diabetes, possibly due to increased shedding from tubular cells. PMID: 22629438
  79. study identified three SNPs (rs1514283, rs4646176, and rs879922)in the ACE2 gene that were significantly associated with diastolic blood pressure responses to cold stress in the Chinese female population PMID: 22647782
  80. Downregulation of ACE2 expression is dependent on the efficiency of HCoV-NL63 replication. PMID: 22718567
  81. The ACE2 gene polymorphism G8790A is probably a genetic risk factor for essential hypertension across different ethnic populations in female subjects and in Han-Chinese male subjects. [Meta-analysis] PMID: 22297693
  82. both ACE and ACE2 bind integrin subunits, in an RGD-independent manner, and that they can act as cell adhesion substrates. We show that cellular expression of ACE2 enhanced cell adhesion PMID: 22523556
  83. MasR was significantly upregulated in colon adenocarcinoma compared with non-neoplastic colon mucosa, which showed little or no expression of it. ACE gene expression and enzymatic activity were also increased in the tumors. PMID: 22048948
  84. Mutations in the receptor-binding domain of SARS-CoV strengthen favorable interactions with two virus-binding hot spots on ACE2. PMID: 22291007
  85. In Caucasians with type 2 diabetes, genetic variation in ACE2 was associated with hypertension and reduced systolic function in men, and hypertension and increased left ventricular mass in women. PMID: 21993363
  86. ACE2 activity increases with increasing vascular tone and in T1D has microvascular or macrovascular disease, indicating that ACE2 may participate as a compensatory mechanism in the regulation of vascular and renal function in patients with T1D PMID: 22179088
  87. These findings complement those found in other studies of single nucleotide polymorphisms in the ACE2 gene in relation to cardiovascular disease risk. PMID: 21490025
  88. the II genotype was found to be significantly associated with NS in the Indian and Malay populations and in the Pakistani population described here. PMID: 22033511
  89. angiotensin-converting enzyme 2 has a role in cardiac hypertrophy induced by nitric oxide synthase inhibition PMID: 21946695
  90. ACE2 overexpression in the paraventricular nucleus has beneficial effects in counteracting Ang II-induced hypertension. PMID: 21952934
  91. Data highlight the importance of increased expression and subsequent interaction of ACE2 and nNOS within the paraventricular nucleus, leading to a reduction in sympathetic outflow in congestive heart failure. PMID: 21963832
  92. The nucleotide sequences -516/-481 of human ace2 may be a binding domain for an as yet unidentified regulatory factor(s) that regulates ace2 expression and is associated with angiotensin II stimulation. PMID: 21864606
  93. Central recombinant human ACE2 overexpression has a protective effect in murine congestive heart failure by attenuating sympathetic outflow thru ngiotensin (1-7) mas signaling & a decrease in angiotensin II type 1 receptor signaling in the medulla. PMID: 22025374
  94. Hypoxia down-regulates the protein and gene expression of ACE2 in HUVECs. PMID: 21265092
  95. Results demonstrate that mouse and rat ACE2 are less sensitive to inhibition by DX600 than human ACE2. PMID: 21880865
  96. Urinary ACE2 is up-regulated in patients with chronic kidney disease. PMID: 21457402
  97. Increased myocardial ACE2 expression in patients with end-stage heart failure is associated with the degree of left ventricular dilatation and may therefore be an adaptive mechanism. PMID: 20060185
  98. ACE and ACE2 may play an important role in the pathogenesis and progression of hypertensive nephrosclerosis PMID: 21346373
  99. Report expression (pro)renin receptors and angiotensin converting enzyme 2/angiotensin-(1-7)/Mas receptor axis in human aortic valve stenosis. PMID: 21316680
  100. The ACE2 gene G9570A polymorphisms may be associated with the occurrence of stroke in essential hypertension patients in South China. PMID: 20117991

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Subcellular Location Processed angiotensin-converting enzyme 2: Secreted, SUBCELLULAR LOCATION: Cell membrane, Single-pass type I membrane protein, Cytoplasm
Protein Families Peptidase M2 family
Tissue Specificity Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidn
Database Links

HGNC: 13557

OMIM: 300335

KEGG: hsa:59272

STRING: 9606.ENSP00000252519

UniGene: Hs.178098

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