Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2 μg/mL can bind Human CRTAM (CSB-MP005998HU1), the EC50 is 17.82-21.71 ng/mL.
Alternative Names
Cell adhesion molecule 1 ; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Spermatogenic immunoglobulin superfamily; SgIgSF; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1
Species
Homo sapiens (Human)
Expression Region
45-374aa
Target Protein Sequence
QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDH
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The gene fragment encoding the 45-374aa of the human CADM1 protein is fused with a 10xHis-tag gene at the C-terminus and then inserted into a plasmid vector. The recombinant vector is introduced into mammalian cells, and the transfected mammalian cells are selected and cultured for protein expression. The product is the recombinant human CADM1 protein. Its purity is greater than 95% determined by SDS-PAGE and its endotoxin level is less than 1.0 EU/ug measured by the LAL method. Its bioactivity is validated in a function ELISA. Immobilized human CADM1 at 2 μg/mL can bind human CRTAM (CSB-MP005998HU1), with the EC50 of 17.82-21.71 ng/mL.
CADM1 is expressed in neurons, mast cells, alveolar cells, and pancreatic secretory cells. It is also expressed in fetal and cirrhotic adult bile duct cells, but absent in healthy adult bile ducts. CADM1 mediates cell-cell adhesion through calcium-independent homophilic interactions, contributing to cell interactions and the formation and maintenance of epithelial structures. Overexpression of CADM1 can inhibit epithelial-mesenchymal transition (EMT) induced by growth factors, while loss of CADM1 function can lead to invasion and metastasis of epithelial-derived cancer cells. CADM1 was initially identified as a tumor suppressor gene in human non-small cell lung cancer through functional complementation assays. Currently, a large body of evidence suggests that CADM1 is involved in the formation and development of various human tumors, and downregulation of CADM1 contributes to tumorigenesis in various cancers. Developing stable and active CADM1 proteins can help further investigate the underlying mechanisms and provide new clues for monitoring, diagnosing, and treating tumors.
