Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2 μg/mL can bind Human CRTAM (CSB-MP005998HU1), the EC50 is 17.82-21.71 ng/mL.

Target Names

CADM1

Uniprot No.

Q9BY67

Alternative Names

Cell adhesion molecule 1 ; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Spermatogenic immunoglobulin superfamily; SgIgSF; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

45-374aa

Target Protein Sequence

QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDH

Mol. Weight

38.5 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The gene fragment encoding the 45-374aa of the human CADM1 protein is fused with a 10xHis-tag gene at the C-terminus and then inserted into a plasmid vector. The recombinant vector is introduced into mammalian cells, and the transfected mammalian cells are selected and cultured for protein expression. The product is the recombinant human CADM1 protein. Its purity is greater than 95% determined by SDS-PAGE and its endotoxin level is less than 1.0 EU/ug measured by the LAL method. Its bioactivity is validated in a function ELISA. Immobilized human CADM1 at 2 μg/mL can bind human CRTAM (CSB-MP005998HU1), with the EC₅₀ of 17.82-21.71 ng/mL.

CADM1 is expressed in neurons, mast cells, alveolar cells, and pancreatic secretory cells. It is also expressed in fetal and cirrhotic adult bile duct cells, but absent in healthy adult bile ducts. CADM1 mediates cell-cell adhesion through calcium-independent homophilic interactions, contributing to cell interactions and the formation and maintenance of epithelial structures. Overexpression of CADM1 can inhibit epithelial-mesenchymal transition (EMT) induced by growth factors, while loss of CADM1 function can lead to invasion and metastasis of epithelial-derived cancer cells. CADM1 was initially identified as a tumor suppressor gene in human non-small cell lung cancer through functional complementation assays. Currently, a large body of evidence suggests that CADM1 is involved in the formation and development of various human tumors, and downregulation of CADM1 contributes to tumorigenesis in various cancers. Developing stable and active CADM1 proteins can help further investigate the underlying mechanisms and provide new clues for monitoring, diagnosing, and treating tumors.

Target Background

Function

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node. Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells. Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. May contribute to the less invasive phenotypes of lepidic growth tumor cells.

Gene References into Functions

miRNA-873 promoted Hepatocellular Carcinoma progression by targeting TSLC1. PMID: 29734164
High CADM1 expression is associated with KSHV infection. PMID: 29698475
the immunohistochemical expression of CADM1 has the potential to contribute as an auxiliary tool to the differential diagnosis between nevi and melanomas. PMID: 28843749
These findings suggest that CADM1 expression is a novel prognostic factor for peripheral T cell lymphoma, not otherwise specified PMID: 28940014
miR-21 enhances chemo-resistance in tongue cancer cells via directly targeting CADM1, and an inverse correlation between miR-21 and CADM1 expression in vivo. MiR-21 overexpression is attributed to MYCN-mediated transcriptional regulation, which is also predictive for a worse prognosis in tongue cancer PMID: 27055844
We conclude that CADM1 was not associated with cell-cell interaction between lymphoma cells and macrophages, although CADM1 may be a useful marker of ATLL for diagnostic procedures. PMID: 28420814
miR-21 overexpression promotes cardiac fibrosis via STAT3 signaling pathway by decrease CADM1 expression, indicating miR-21 as an important signaling molecule for cardiac fibrotic remodeling and atrial fibrillation. PMID: 28335740
Pathway analysis of the genes associated with the 46 CpG sites revealed an enrichment of immune system process genes, including LYST (cg16962115, FDR = 1.24E-04), CADM1 (cg21933078, FDR = 1.22E-02) and NFATC1 (cg06784563, FDR = 1.46E-02) PMID: 28637314
CADM1 mRNA expression was highly upregulated in normal tissues compared to esophageal squamous cell carcinoma tissues, indicating that the loss of CADM1 expression influenced the pathogenesis, invasion, and metastasis of ESCC. PMID: 28510248
Eight and five of the nine samples were negative for cell adhesion molecule 1 and Osterix respectively. The other markers showed no statistical significance(CD151,ALP). osteoblastic differentiation can occur in carcinoma cells and that cell adhesion molecule 1 could be a useful marker for identifying this phenomenon in carcinoma tissues PMID: 28651491
Our results suggest that, as in the CNS, CADM1 interactions drive exocytic site assembly and promote actin network formation. These results support the broader hypothesis that the effects of cell-cell contact on beta-cell maturation and function are mediated by the same extracellular protein interactions that drive the formation of the presynaptic exocytic machinery. These interactions may be therapeutic targets for re... PMID: 27072493
High expression of CADM1 on most HTLV-1-infected cells in the face of enhanced cytotoxic T lymphocyte counterselection implies that CADM1 confers a strong benefit on the virus. PMID: 27105228
Decreased expression of TSLC1 is associated with the differentiation in ovarian epithelial cancer. TSLC1 might be used as a molecular marker of severity in early stage ovarian cancer, and to help differentiating benign and malignant ovarian tumors. PMID: 27146728
Suppression of tumorigenesis by CADM1 may be mediated by the Rb-E2F1 pathway. PMID: 27298105
CADM1 prevents squamous cell carcinoma progression by reducing STAT3 activity PMID: 27035095
Decreased expression of TSLC1 is correlated with the malignancy of non-muscle invasive bladder cancer tissues and low TSLC1 expression may serve as a predictor for bladder cancer recurrence and progression. PMID: 26894268
CADM1 deletion is associated with neuroblastoma and ganglioneuroma. PMID: 26463893
Hypermethylation of CADM1 gene may be highly associated with the development of cervical cancer. PMID: 26552048
Our study showed that miR-126, by down-regulation CADM1, enhances migration and invasion in gastric cancer cells. PMID: 26464628
This study revealed that Merkel cell polyomavirus -negative Merkel cell carcinomas significantly expressed higher CADM1 and lower MAL than Merkel cell polyomavirus -positive Merkel cell carcinomas PMID: 26772392
CADM1/MAL methylation increases with severity of cervical intraepithelial neoplasia PMID: 26219541
SynCAM1 delineates the postsynaptic perimeter of the synaptic cleft as determined by immunoelectron microscopy. PMID: 26687224
increased CADM1 shedding appeared to contribute to the development of emphysema by generating alphaCTF and the ICD in lung epithelial cells. PMID: 26259600
CADM1 shedding is increased in idiopathic interstitial pneumonia and contributes to alveolar epithelial cell apoptosis. PMID: 26231557
Results show that CADM1 is downregulated in HPV-positive cervical cancers and that epigenetic alteration of CADM1 was more frequent in this type of cancer. The restoration of CADM1 expression may be a potential strategy for cervical cancer therapy. PMID: 25845528
The use of CADM1, MAL, and MIR124-2 as biomarkers for full molecular screening in HIV infected women who are also positive for human papillomavirus. PMID: 26473640
CADM1 induction was repressed in primary samples from AML patients at relapse. PMID: 25491945
Our study reveals a novel mechanism of chronic NF-kappaB activation by CADM1 in HTLV-1 infected cells PMID: 25774694
Trans-homophilic interaction of CADM1 activates PI3K by forming a complex with MAGuK-family proteins MPP3 and Dlg. PMID: 25268382
In the absence of SynCAMs, selective axon-axon interactions are perturbed resulting in aberrant pathfinding of sensory axons. PMID: 25335893
Increased ectodomain shedding of cell adhesion molecule 1 from pancreatic islets in type 2 diabetic pancreata. PMID: 24964098
In a population-based association study, we confirm a role for CADM1 variants in increasing the risk of VT by interaction with protein C pathway abnormalities. PMID: 25306186
The significant association of IGSF4 with HPV-positive head and neck squamous cell carcinoma suggests that HPV-positive tumors are to a greater extent driven by promoter hypermethylation in this tumor suppressor gene. PMID: 25569158
HPV-positive oropharyngeal squamous cell carcinoma is associated with TIMP3 and CADM1 promoter hypermethylation. PMID: 25065733
CADM1/TSLC1 is an antiinvasive gene, the overexpression of which inhibits the invasion of A375 cells. PMID: 25215547
Cytology and bi-marker CADM1/MAL-methylation analysis perform complementary for CIN2+/CIN3+ detection when used as triage tool on cervical scrapes of HPV positive women. PMID: 25667975
TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. PMID: 25031725
The study showed that CADM1 is a direct target of miR-1246 leading to its down regulation in hepatocellular carcinoma cells. PMID: 25159494
DNA methylation analysis of CADM1, MAL and miR124-2 in cervical scrapes consistently detects cervical cancer PMID: 25281766
Loss of CADM1 expression is an independent prognostic factor especially associated with the development of brain metastases in breast cancer patients. PMID: 24833255
We showed that the CADM1 versus CD7 plot is capable of discriminating clonally expanding HTLV-I-infected cells in indolent and acute-type T-cell leukemia-lymphomas and in asymptomatic carriers PMID: 24727323
MPP3 and Dlg, membrane-associated guanylate kinase homologs (MAGuK) proteins, connect CADM1 with p85 of PI3K by forming a multi-protein complex at the periphery of cells. PMID: 24503895
Data suggest that cell adhesion molecule 1 (CADM1) is a key adhesion receptor, which regulates mast cells (MCs) net adhesion. PMID: 24465823
TGF-beta upregulates miR-182 expression to promote gallbladder cancer metastasis by targeting CADM1. PMID: 24445397
CADM1 ectodomain shedding appeared to cause alveolar cell apoptosis in emphysematous lungs by producing alphaCTF that accumulated in mitochondria. PMID: 24092566
The paper shows that CADM1 regulates mast cell net adhesion, both directly through CADM1-dependent adhesion, and indirectly through the regulation of other adhesion receptors. The latter is likely to occur via docking of KIT and polymerization of cortical F-actin. CADM1 is involved in docking of KIT to the cell surface and phosphotyrisine signaling, and polymerization of cortical F-actin. PMID: 24465823
SynCAM, a novel putative tumor suppressor, suppresses growth and invasiveness of glioblastoma. PMID: 23907434
Infection of NCI-H460, A549, and H1299 lung cancer cells with Ad.sp-E1A(Delta24)-TSLC1 induced apoptosis. PMID: 23503473
Data suggest that tumor suppressor in lung cancer 1 (TSLC1) as tumor suppressor may play prominent roles in development and progression of cutaneous squamous cell carcinoma (CSCC) via regulation of different biological processes. PMID: 23812766
The cell adhesion molecule Necl-2 competitively binds the immune receptor CRTAM. PMID: 23871486

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cell junction, synapse.

Protein Families

Nectin family

Database Links

HGNC: 5951

OMIM: 605686

KEGG: hsa:23705

STRING: 9606.ENSP00000395359

UniGene: Hs.370510

Code	CSB-MP004425HU
Abbreviation	Recombinant Human CADM1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$102
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2μg/mL can bind Human CRTAM (CSB-MP005998HU1)，the EC₅₀ is 17.82-21.71 ng/mL. Biological Activity Assay
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Recombinant Human Cell adhesion molecule 1 (CADM1), partial (Active)

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