Recombinant Human G-protein coupled receptor 20 (GPR20)-VLPs (Active)

GPR20 remains an orphan G-protein coupled receptor with poorly defined ligand specificity, making validated full-length constructs essential for deorphanization efforts and functional characterization. This mammalian-expressed construct spans the complete 358-residue sequence and demonstrates specific antibody binding in functional ELISA with an EC50 of 2.431–3.239 ng/mL, confirming that native epitopes are accessible and properly folded. The quantified binding kinetics support use in therapeutic antibody epitope mapping, competitive inhibition screening to identify blocking antibodies, and ligand-binding assays designed to identify endogenous or synthetic agonists. Endotoxin levels below 1.0 EU/μg meet the contamination thresholds commonly required for cell-based functional assays and receptor-ligand interaction studies by surface plasmon resonance or biolayer interferometry, where low background interference is critical for accurate affinity measurements.