GPR20 Recombinant Monoclonal Antibody

GPR20 is an orphan G protein-coupled receptor that has garnered increasing attention in cancer research due to its restricted expression pattern in normal tissues and elevated presence in certain malignancies. This expression profile positions GPR20 as a potentially valuable target for therapeutic development and diagnostic applications, making reliable detection tools essential for researchers investigating this receptor's biology and clinical significance.

This recombinant monoclonal antibody against GPR20 offers the consistency and reproducibility that demanding research applications require. Produced using recombinant technology with a defined sequence, this clone 2C1 antibody eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The human IgG1 format and affinity-chromatography purification ensure high purity, as confirmed by SEC-HPLC analysis demonstrating greater than 95% purity.

Functional ELISA validation demonstrates robust binding activity against both human and Macaca fascicularis GPR20, providing researchers with cross-species reactivity that supports translational studies involving non-human primate models. The antibody exhibits excellent sensitivity, with EC50 values of 2.431 to 3.239 ng/mL for human GPR20 and 3.549 to 6.542 ng/mL for the cynomolgus macaque ortholog. For ELISA applications, dilutions ranging from 1:5000 to 1:50000 are recommended, offering flexibility to optimize signal-to-noise ratios across different experimental conditions.

Supplied in a stabilizing buffer containing 50% glycerol and PBS at pH 7.4, this antibody maintains activity through proper storage at -20°C or -80°C. Researchers exploring GPR20 as a potential immunotherapy target, investigating its role in tumor biology, or developing companion diagnostics will find this antibody a dependable tool for their discovery efforts.