Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 3.049-4.660 ng/mL.
Research Area
Microbiology
Alternative Names
(STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(Intestinal guanylate cyclase)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
24-430aa
Target Protein Sequence
SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ
Tag Info
C-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO's product CSB-MP010053HUd9 is a recombinant human heat-stable enterotoxin receptor (GUCY2C) labeled with a human Fc tag at the C-terminus. It is generated through the expression of the gene fragment encoding amino acids 24-430 of the human GUCY2C in mammalian cells. The purity of this recombinant GUCY2C protein is greater than 90% determined by the SDS-PAGE. On the gel, there appeared a band with molecular weight ranging from 90 kDa to 116 kDa. The endotoxin of this protein is less than 1.0 EU/ug measured by the LAL method. Moreover, this recombinant protein has also been validated to be active. In the functional ELISA, this GUCY2C protein can bind to the anti-GUCY2C recombinant antibody. The EC50 is 3.049-4.660 ng/mL. This GUCY2C protein is available now.
GUCY2C is a transmembrane receptor exclusively expressed in the intestinal epithelium. High expression of GUCY2C has been found all along the rostral-caudal axis. Activated by three cognate ligands guanylin, uroguanylin, and ST, GUCY2C promotes the conversion of GTP to cGMP, which increases intracellular cGMP levels, leading to activation of downstream effectors, including PKGs, PDEs, and CNG channels, thus regulating electrolyte and fluid balance, promoting proliferation and differentiation along the intestinal crypt-villus axis, sustaining genomic integrity via DNA damage repair, and modulating cellular metabolism and the tight junctional barrier function of the intestinal epithelium. All these physiologic processes ultimately help to maintain intestinal homeostasis.