Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active)

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Code CSB-MP010053HUd9
Size $228
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/ml can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 3.049-4.660 ng/mL. Biological Activity Assay
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Product Details

Greater than 90% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 3.049-4.660 ng/mL.
Target Names
Uniprot No.
Research Area
Alternative Names
(STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(Intestinal guanylate cyclase)
Molecular Characterization
Homo sapiens (Human)
Mammalian cell
Expression Region
Target Protein Sequence
Mol. Weight
74.9 kDa
Protein Length
Tag Info
C-terminal hFc-tagged
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

CUSABIO's product CSB-MP010053HUd9 is a recombinant human heat-stable enterotoxin receptor (GUCY2C) labeled with a human Fc tag at the C-terminus. It is generated through the expression of the gene fragment encoding amino acids 24-430 of the human GUCY2C in mammalian cells. The purity of this recombinant GUCY2C protein is greater than 90% determined by the SDS-PAGE. On the gel, there appeared a band with molecular weight ranging from 90 kDa to 116 kDa. The endotoxin of this protein is less than 1.0 EU/ug measured by the LAL method. Moreover, this recombinant protein has also been validated to be active. In the functional ELISA, this GUCY2C protein can bind to the anti-GUCY2C recombinant antibody. The EC50 is 3.049-4.660 ng/mL. This GUCY2C protein is available now.

GUCY2C is a transmembrane receptor exclusively expressed in the intestinal epithelium. High expression of GUCY2C has been found all along the rostral-caudal axis. Activated by three cognate ligands guanylin, uroguanylin, and ST, GUCY2C promotes the conversion of GTP to cGMP, which increases intracellular cGMP levels, leading to activation of downstream effectors, including PKGs, PDEs, and CNG channels, thus regulating electrolyte and fluid balance, promoting proliferation and differentiation along the intestinal crypt-villus axis, sustaining genomic integrity via DNA damage repair, and modulating cellular metabolism and the tight junctional barrier function of the intestinal epithelium. All these physiologic processes ultimately help to maintain intestinal homeostasis.

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Target Background

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.
Gene References into Functions
  1. High Expressions of guanylyl cyclase C is associated with rectal cancer. PMID: 28418917
  2. The findings support that the activating mutation in GUCY2C creates an intestinal environment with a major influence on the microbiota, which could contribute to the increased susceptibility to inflammatory bowel disease in patients with Familial GUCY2C diarrhea syndrome. PMID: 28902124
  3. the expression of GCC is maintained throughout the process of tumor progression and formation of metastatic disease. PMID: 29261789
  4. To investigate gut motility and hormones before and after a meal in Familial GUCY2C diarrhea syndrome patients and compare with healthy controls PMID: 28957388
  5. Mutations in GUCY2C indicate a role for this receptor in the pathogenesis of sporadic Congenital secretory diarrhea. PMID: 25994218
  6. these data support Ad5-GUCY2C-PADRE as a safe and effective vaccination strategy in preclinical models and position Ad5-GUCY2C-PADRE for Phase I clinical testing in colorectal cancer patients. PMID: 27903079
  7. Familial GUCY2C diarrhoea syndrome is caused by an activating mutation in the GUCY2C gene, which causes impaired contractility and fluid stagnation in the small bowel PMID: 27338166
  8. Findings show how caloric suppression of the guanylin-GUCY2C signaling axis links obesity to negation of a universal tumor suppressor pathway in colorectal cancer. PMID: 26773096
  9. Data show that individuals affected with meconium ileus (MI) had either homozygous or compound heterozygous variants in enterotoxin receptor GUCY2C. PMID: 25370039
  10. High guanylyl cyclase C expression is associated with colon cancer recurrence. PMID: 24919572
  11. Transmission disequilibrium tests (TDT), case-control studies and quantitative analyses indicated association between GUC2C with Attention-deficit/hyperactivitydisorder and its core symptoms. PMID: 25064385
  12. Intestinal cell proliferation and senescence are regulated by receptor guanylyl cyclase C and p21. PMID: 24217248
  13. GUCY2C signaling constitutes a direct link between the initiation of colorectal cancer and the induction of its associated desmoplastic stromal niche. PMID: 24085786
  14. Data show that lower survival (OS) and disease-free survival (DFS) rates were significantly associated with guanylate cyclase C (GCC) and CK20 mRNA levels. PMID: 23150200
  15. Interaction with VIP36 is dependent on glycosylation at the same sites that allow GC-C to fold and bind ligand. PMID: 23269669
  16. GUCY2C opposes systemic genotoxic tumorigenesis by regulating AKT-dependent intestinal barrier integrity PMID: 22384056
  17. Mutations in GUCY2C were associated with the Meconium ileus phenotype. downregulation of GUCY2C leads to a cystic fibrosis-like intestinal phenotype. PMID: 22521417
  18. We identified a heterozygous missense mutation (c.2519G-->T) in GUCY2C in all family members with familial diarrhea syndrome. PMID: 22436048
  19. High guanylyl cyclase C is associated with colon cancer. PMID: 21533822
  20. Data suggest that monitoring the peripheral blood GCC expressions may allow employing different treatment options to metastatic CRC patients. PMID: 21901559
  21. Lymph nodes (range: 2-159) from 291 prospectively enrolled node-negative colorectal cancer patients were analyzed by histopathology and GUCY2C quantitative RT-PCR. PMID: 21307149
  22. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
  23. A novel PDZ protein regulates the activity of guanylyl cyclase C, the heat-stable enterotoxin receptor. PMID: 11950846
  24. guanylyl cyclase c is ectopically expressed in primary and metastatis adenocarcinoma of the esophagus and stomach PMID: 12163327
  25. SH3 domain -mediated protein-protein interaction with the catalytic domain of guanylyl cyclase C inhibited the cyclase activity and might be pivotal for the desensitization phenomenon of the guanylyl cyclase C receptor PMID: 12649275
  26. gelatinolytic activity of GCC in colorectal tumor tissues may facilitate the hepatic metastatic process in the steps after intravasation but not during or before intravasation PMID: 14581363
  27. a novel role for glycosidic modification of GC-C during its biosynthesis, in imparting subtle conformational changes in the receptor that allow for ligand-mediated activation and perhaps regulation of basal activity PMID: 14748740
  28. Lack of guanylyl cyclase c expression assoeciated with significant reduction in adenomas in Apc(Min/+);Gucy2c(-/-) mice PMID: 15825168
  29. Transformation associated with reflux at the gastroesophageal junction reflects activation by bile acid and acid of a transcriptional program involving NF-kappaB and Cdx2, which mediate intestinal metaplasia and ectopic expression of GC-C. PMID: 16618413
  30. This validated assay is being applied to approximately 10,000 lymph nodes in a prospective trial to define the sensitivity of Guanylyl cyclase C(GCC) qRT-PCR for staging patients with colorectal cancer. PMID: 16899600
  31. docking of the c-src SH2 domain to phosphorylated GC-C results in colocalization and further activation of c-src. PMID: 19620276
  32. the role of the linker region in receptor guanylyl cyclases PMID: 19648115
  33. GCC is a specific marker for differentiating primary and secondary metatstatic ovarian mucinous neoplasms. PMID: 19694825
  34. Results suggest that GUCY2C quantitative reverse transcriptase (qRT)-PCR can be used to detect occult lymph node metastases in pN0 colorectal cancer patients. PMID: 19895223
  35. Expression of the receptor guanylyl cyclase C and its ligands in reproductive tissues of the rat: a potential role for a novel signaling pathway in the epididymis. PMID: 12444076
  36. The protein kinase-like domain in receptor guanylyl cyclase C provides a critical structural link between the extracellular domain and the catalytic domain in regulation of this family of receptors. PMID: 11478887

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Involvement in disease
Diarrhea 6 (DIAR6); Meconium ileus (MECIL)
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.
Protein Families
Adenylyl cyclase class-4/guanylyl cyclase family
Database Links

HGNC: 4688

OMIM: 601330

KEGG: hsa:2984

STRING: 9606.ENSP00000261170

UniGene: Hs.524278

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