Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC50 is 11.89-24.13 ng/mL.
Research Area
Microbiology
Alternative Names
(STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(Intestinal guanylate cyclase)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
24-430aa
Target Protein Sequence
SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ
Tag Info
N-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant human GUCY2C protein (24-430 AA) was fused to a 10xHis-tag at the N-terminus and expressed in the mammalian cells. This GUCY2C protein is characterized by high purity (>95%, SDS-PAGE), low endotoxin content (<1.0 EU/ug of the protein, LAL method), and biological activity. Its activity has been validated by binding to the anti-GUCY2C recombinant antibody in a functional ELISA, with the EC50 of 11.89-24.13 ng/mL. The GUCY2C protein has a calculated molecular weight of 49.6 kDa. It migrates as 60-90 kDa under reducing conditions (SDS-PAGE) due to glycosylation. And it is available now.
GUCY2C, an intestine-specific expressed transmembrane protein, participates in regulating intestinal function and Na+ dynamic balance, and promoting food digestion and absorption after being activated by STa, guanosine, and uroguanosine. GUCY2C is considered a colorectal cancer-specific antigen due to its high intestinal tissue specificity. To date, the GUCY2C protein has been used in vaccines, immunotoxins, and CAR-T cells, among other immunotherapies. Some trials have achieved good clinical results. The development and research of GUCY2C recombinant protein can promote and push the development of vaccines and treatment of GUCY2C-expressing tumors of the intestine.