Name: Recombinant Human Heat-stable enterotoxin receptor(GUCY2C),partial (Active)
Brand: CUSABIO
SKU: CSB-MP010053HU

Product Details

Description	Recombinant human GUCY2C protein (24-430 AA) was fused to a 10xHis-tag at the N-terminus and expressed in the mammalian cells. This GUCY2C protein is characterized by high purity (>95%, SDS-PAGE), low endotoxin content (<1.0 EU/ug of the protein, LAL method), and biological activity. Its activity has been validated by binding to the anti-GUCY2C recombinant antibody in a functional ELISA, with the EC₅₀ of 11.89-24.13 ng/mL. The GUCY2C protein has a calculated molecular weight of 49.6 kDa. It migrates as 60-90 kDa under reducing conditions (SDS-PAGE) due to glycosylation. And it is available now. GUCY2C, an intestine-specific expressed transmembrane protein, participates in regulating intestinal function and Na+ dynamic balance, and promoting food digestion and absorption after being activated by STa, guanosine, and uroguanosine. GUCY2C is considered a colorectal cancer-specific antigen due to its high intestinal tissue specificity. To date, the GUCY2C protein has been used in vaccines, immunotoxins, and CAR-T cells, among other immunotherapies. Some trials have achieved good clinical results. The development and research of GUCY2C recombinant protein can promote and push the development of vaccines and treatment of GUCY2C-expressing tumors of the intestine.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/mL can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC₅₀ is 11.89-24.13 ng/mL.
Target Names	GUCY2C
Uniprot No.	P25092
Research Area	Microbiology
Alternative Names	(STA receptor)(hSTAR)(Guanylyl cyclase C)(GC-C)(Intestinal guanylate cyclase)
Molecular Characterization
Species	Homo sapiens (Human)
Source	Mammalian cell
Expression Region	24-430aa
Target Protein Sequence	SQVSQNCHNGSYEISVLMMGNSAFAEPLKNLEDAVNEGLEIVRGRLQNAGLNVTVNATFMYSDGLIHNSGDCRSSTCEGLDLLRKISNAQRMGCVLIGPSCTYSTFQMYLDTELSYPMISAGSFGLSCDYKETLTRLMSPARKLMYFLVNFWKTNDLPFKTYSWSTSYVYKNGTETEDCFWYLNALEASVSYFSHELGFKVVLRQDKEFQDILMDHNRKSNVIIMCGGPEFLYKLKGDRAVAEDIVIILVDLFNDQYFEDNVTAPDYMKNVLVLTLSPGNSLLNSSFSRNLSPTKRDFALAYLNGILLFGHMLKIFLENGENITTPKFAHAFRNLTFEGYDGPVTLDDWGDVDSTMVLLYTSVDTKKYKVLLTYDTHVNKTYPVDMSPTFTWKNSKLPNDITGRGPQ
Mol. Weight	49.6 kDa
Protein Length	Partial
Tag Info	N-terminal 10xHis-tagged
Form	Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	3-7 business days
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function	Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.
Gene References into Functions	High Expressions of guanylyl cyclase C is associated with rectal cancer. PMID: 28418917 The findings support that the activating mutation in GUCY2C creates an intestinal environment with a major influence on the microbiota, which could contribute to the increased susceptibility to inflammatory bowel disease in patients with Familial GUCY2C diarrhea syndrome. PMID: 28902124 the expression of GCC is maintained throughout the process of tumor progression and formation of metastatic disease. PMID: 29261789 To investigate gut motility and hormones before and after a meal in Familial GUCY2C diarrhea syndrome patients and compare with healthy controls PMID: 28957388 Mutations in GUCY2C indicate a role for this receptor in the pathogenesis of sporadic Congenital secretory diarrhea. PMID: 25994218 these data support Ad5-GUCY2C-PADRE as a safe and effective vaccination strategy in preclinical models and position Ad5-GUCY2C-PADRE for Phase I clinical testing in colorectal cancer patients. PMID: 27903079 Familial GUCY2C diarrhoea syndrome is caused by an activating mutation in the GUCY2C gene, which causes impaired contractility and fluid stagnation in the small bowel PMID: 27338166 Findings show how caloric suppression of the guanylin-GUCY2C signaling axis links obesity to negation of a universal tumor suppressor pathway in colorectal cancer. PMID: 26773096 Data show that individuals affected with meconium ileus (MI) had either homozygous or compound heterozygous variants in enterotoxin receptor GUCY2C. PMID: 25370039 High guanylyl cyclase C expression is associated with colon cancer recurrence. PMID: 24919572 Transmission disequilibrium tests (TDT), case-control studies and quantitative analyses indicated association between GUC2C with Attention-deficit/hyperactivitydisorder and its core symptoms. PMID: 25064385 Intestinal cell proliferation and senescence are regulated by receptor guanylyl cyclase C and p21. PMID: 24217248 GUCY2C signaling constitutes a direct link between the initiation of colorectal cancer and the induction of its associated desmoplastic stromal niche. PMID: 24085786 Data show that lower survival (OS) and disease-free survival (DFS) rates were significantly associated with guanylate cyclase C (GCC) and CK20 mRNA levels. PMID: 23150200 Interaction with VIP36 is dependent on glycosylation at the same sites that allow GC-C to fold and bind ligand. PMID: 23269669 GUCY2C opposes systemic genotoxic tumorigenesis by regulating AKT-dependent intestinal barrier integrity PMID: 22384056 Mutations in GUCY2C were associated with the Meconium ileus phenotype. downregulation of GUCY2C leads to a cystic fibrosis-like intestinal phenotype. PMID: 22521417 We identified a heterozygous missense mutation (c.2519G-->T) in GUCY2C in all family members with familial diarrhea syndrome. PMID: 22436048 High guanylyl cyclase C is associated with colon cancer. PMID: 21533822 Data suggest that monitoring the peripheral blood GCC expressions may allow employing different treatment options to metastatic CRC patients. PMID: 21901559 Lymph nodes (range: 2-159) from 291 prospectively enrolled node-negative colorectal cancer patients were analyzed by histopathology and GUCY2C quantitative RT-PCR. PMID: 21307149 Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013 A novel PDZ protein regulates the activity of guanylyl cyclase C, the heat-stable enterotoxin receptor. PMID: 11950846 guanylyl cyclase c is ectopically expressed in primary and metastatis adenocarcinoma of the esophagus and stomach PMID: 12163327 SH3 domain -mediated protein-protein interaction with the catalytic domain of guanylyl cyclase C inhibited the cyclase activity and might be pivotal for the desensitization phenomenon of the guanylyl cyclase C receptor PMID: 12649275 gelatinolytic activity of GCC in colorectal tumor tissues may facilitate the hepatic metastatic process in the steps after intravasation but not during or before intravasation PMID: 14581363 a novel role for glycosidic modification of GC-C during its biosynthesis, in imparting subtle conformational changes in the receptor that allow for ligand-mediated activation and perhaps regulation of basal activity PMID: 14748740 Lack of guanylyl cyclase c expression assoeciated with significant reduction in adenomas in Apc(Min/+);Gucy2c(-/-) mice PMID: 15825168 Transformation associated with reflux at the gastroesophageal junction reflects activation by bile acid and acid of a transcriptional program involving NF-kappaB and Cdx2, which mediate intestinal metaplasia and ectopic expression of GC-C. PMID: 16618413 This validated assay is being applied to approximately 10,000 lymph nodes in a prospective trial to define the sensitivity of Guanylyl cyclase C(GCC) qRT-PCR for staging patients with colorectal cancer. PMID: 16899600 docking of the c-src SH2 domain to phosphorylated GC-C results in colocalization and further activation of c-src. PMID: 19620276 the role of the linker region in receptor guanylyl cyclases PMID: 19648115 GCC is a specific marker for differentiating primary and secondary metatstatic ovarian mucinous neoplasms. PMID: 19694825 Results suggest that GUCY2C quantitative reverse transcriptase (qRT)-PCR can be used to detect occult lymph node metastases in pN0 colorectal cancer patients. PMID: 19895223 Expression of the receptor guanylyl cyclase C and its ligands in reproductive tissues of the rat: a potential role for a novel signaling pathway in the epididymis. PMID: 12444076 The protein kinase-like domain in receptor guanylyl cyclase C provides a critical structural link between the extracellular domain and the catalytic domain in regulation of this family of receptors. PMID: 11478887 Show More Hide All
Involvement in disease	Diarrhea 6 (DIAR6); Meconium ileus (MECIL)
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.
Protein Families	Adenylyl cyclase class-4/guanylyl cyclase family
Database Links	HGNC: 4688 OMIM: 601330 KEGG: hsa:2984 STRING: 9606.ENSP00000261170 UniGene: Hs.524278

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Code	CSB-MP010053HU
Size	$428
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized human GUCY2C at 5 μg/ml can bind Anti-GUCY2C recombinant antibody (CSB-RA010053A2HU), the EC₅₀ is 11.89-24.13 ng/mL. Biological Activity Assay
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Recombinant Human Heat-stable enterotoxin receptor(GUCY2C),partial (Active)

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