Code | CSB-RA010053A3HU |
Size | US$210 |
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Application | Recommended Dilution |
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FC | 1:50-1:200 |
The synthesis of the GUCY2C recombinant monoclonal antibody follows a meticulous process to ensure its exceptional quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, using the recombinant human GUCY2C protein as the immunogen. RNA is then extracted from the B cells and converted into cDNA through reverse transcription. The GUCY2C antibody genes are amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, enabling the production of the GUCY2C recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. Rigorous characterization assays, including ELISA and FC analysis, are performed to validate the antibody's specificity and functionality in detecting human GUCY2C protein.
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