Code | CSB-MP764932HU |
MSDS | |
Size | $318 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Recombinant human IGFL1 protein is produced by a mammalian expression system. The target protein is expressed with a DNA fragment (Ala25-Ser110) of human IGFL1 fused with a hFc tag at the C-terminus. This active IGFL1 protein has been measured its activity in a functional ELISA. Immobilized human IGFLR1 binds to this IGFL1 protein with EC50 of 32.33-47.52 ng/mL. The SDS-PAGE showed that the purity of this IGFL1 protein was greater than 95%. The IGFL1 protein has an apparent molecular weight of approximately 40 kDa due to glycosylation. The LAL method showed that the protein contained endotoxin less than 1.0 EU/ug. This human IGFL1 protein is in stock now.
IGFL1 is expressed in cancer cell lines, fetal skin, head and neck tumors, normal breast, squamous cell carcinoma, and uterine tumors. IGFL1 expression is up-regulated in human psoriasis skin. Studies showed that human IGFL1 produced may regulate T cell response. Xiaorong Ji et al. found that high IGFL1 expression can be used as an independent prognostic factor of serous ovarian carcinoma.
Applications : Binding assay/Protein-protein interaction
Review: I used IGFL1 (CSB-MP764932HU) and another product Human IGFLR1 (CSB-MP862025HU) I purchased for functional ELISA experiments, which showed good binding activity, and the EC50 was 4.640-5.722 ng/mL. There is no significant difference between different batches, and the repeatability is good!
By Anonymous