Recombinant Human SIN3-HDAC complex-associated factor (SINHCAF) (Active)

SINHCAF functions as a component of the SIN3-HDAC chromatin remodeling complex and has emerged as a regulator of gene repression programs implicated in cancer biology. This full-length recombinant human SINHCAF (aa 1–221), expressed in *E. coli* with an N-terminal GST tag, demonstrates validated binding to immobilized LCN2 in a functional ELISA, yielding an EC50 of 31.54–38.59 μg/ml—a result that supports use in protein-protein interaction assays, binding competition studies, and as a positive control in ELISA-based screening platforms. The protein's greater than 85% purity by SDS-PAGE provides a suitable basis for biochemical pull-down experiments and antibody development campaigns targeting SINHCAF in oncology research. Researchers should note that endotoxin levels have not been tested, so additional endotoxin removal steps may be necessary before any cell-based functional assays.