Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.230-1.519 ng/mL.
Alternative Names
(5T4 oncofetal antigen)(5T4 oncofetal trophoblast glycoprotein)(5T4 oncotrophoblast glycoprotein)(M6P1)(Wnt-activated inhibitory factor 1)(WAIF1)
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
32-355aa
Target Protein Sequence
SSPTSSASSFSSSAPFLASAVSAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPAYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRAGAFEHLPSLRQLDLSHNPLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS
Tag Info
N-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Human TPBG recombinant protein was produced in mammalian cell, where the gene sequence encoding Human TPBG (32-355aa) was expressed with the N-terminal 10xHis tag. The purity of this TPBG protein was greater than 95%. The activity was validated.
TPBG was first discovered in embryonic development. It is highly expressed in placental trophoblast cells and is basically absent in normal tissues. It only exists in some special epithelia, such as basal stratified squamous epithelium, glandular and ductal epithelium, and retina. Secondary neurons and olfactory bulbs. Later, it was found that TPBG is expressed in many cancers, including uterine cancer, ovarian cancer, colorectal cancer, gastric cancer, kidney cancer, prostate cancer and so on. In colon, gastric, or ovarian cancer, there is evidence that the expression of TPBG is associated with low cancer cure rates. In the tissues of non-small cell lung cancer, kidney cancer or pancreatic cancer, the expression of TPBG is as high as more than 95%. Overexpression of TPBG in tumors is associated with disease progression. It may play a role in affecting the connections between tumor cells, affecting cell morphology, motility and adhesion, and then promoting distant metastasis.
