TPBG Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA902876A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate All lanes: 5T4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47 kDa Observed band size: 50, 80 kDa IHC image of CSB-RA902876A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q13641

Target Names

TPBG

Alternative Names

Trophoblast glycoprotein (5T4 oncofetal antigen) (5T4 oncofetal trophoblast glycoprotein) (5T4 oncotrophoblast glycoprotein) (M6P1) (Wnt-activated inhibitory factor 1) (WAIF1), TPBG, 5T4

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human 5T4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6E9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Trophoblast glycoprotein, commonly known as 5T4 or TPBG, serves as a compelling target for researchers investigating oncofetal biology, tumor progression, and stem cell dynamics. This leucine-rich repeat glycoprotein shows restricted expression in normal adult tissues but becomes markedly upregulated across numerous carcinomas, making it an attractive focus for cancer immunotherapy development and tumor biology studies. Its role as a Wnt-activated inhibitory factor further positions 5T4 at the intersection of developmental signaling and malignant transformation.

This recombinant monoclonal antibody, generated from clone 6E9 in rabbit host, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production eliminates lot-to-lot variability, ensuring your optimization efforts translate reliably across extended studies and collaborative projects.

Validation testing demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects 5T4 in diverse human cancer cell lines including U-87, A549, MCF-7, PC-3, and U-251, as well as mouse brain tissue, confirming cross-species reactivity. The observed bands at approximately 50 and 80 kDa, compared to the predicted 47 kDa molecular weight, reflect the extensive glycosylation characteristic of this trophoblast glycoprotein. The 50 kDa band represents a partially glycosylated precursor protein that exists in the endoplasmic reticulum or Golgi apparatus and has not completed all glycosylation modifications. The 80 kDa band represents a fully glycosylated mature membrane protein, which is the functional active form located on the cell membrane surface. Immunohistochemistry validation in human placenta tissue, where 5T4 is physiologically expressed, confirms suitability for tissue-based studies with recommended dilutions between 1:50 and 1:200.

Whether you are characterizing 5T4 expression in tumor specimens, investigating its function in epithelial-mesenchymal transition, or developing immunotherapeutic strategies, this antibody provides a dependable tool for cancer research, developmental biology, immunology, and stem cell applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Gene References into Functions

In extravillous trophoblasts, 5T4 may function in epithelial-to-mesenchymal transition during placentation. The role of syncytiotrophoblast 5T4 is unknown, but its abundance in shed syncytial vesicles may signify route of sensitization of the maternal immune system. PMID: 28481180
Collectively, these findings demonstrate that an anti-5T4 antibody-drug conjugate reduces the fraction of cancer stem cells (CSC), and prevents local recurrence and suggest a novel therapeutic approach for patients with head and neck squamous cell carcinoma. PMID: 27780858
We obtained GWS evidence (P < 2.7 x 10(-6)) for gene-based association in the total sample with a novel locus, TPBG (P = 1.8 x 10(-6)). PMID: 28183528
In conclusion, the present study provided evidence that TPBG is involved in PDAC metastasis, and that TPBG and its associated signaling pathways may be a suitable target for pancreatic ductal adenocarcinoma therapy. PMID: 25738465
5T4 expression is mechanistically associated with the directional movement of cells through epithelial mesenchymal transition, facilitation of CXCL12/CXCR4 chemotaxis, blocking of canonical Wnt/beta-catenin while favouring non-canonical pathway signalling PMID: 25066861
Tyrosine 325 plus the leucine-rich repeat 1 surface centered on a second exposed aromatic residue, phenylalanine 97, are essential for inhibition of Wnt/beta-catenin signaling. PMID: 24582434
The surface expression of 5T4 marks the use of the CXCR4 rather than the CXCR7 receptor, with distinct consequences for CXCL12 exposure. PMID: 22956548
5T4 is a potential new antigen for targeted therapies such as immunotherapy in MPM, as it is overexpressed on mesothelioma cells and recognised by 5T4-specific cytotoxic T-cells. PMID: 22498111
Glycosylation and epitope mapping of the 5T4 glycoprotein oncofoetal antigen PMID: 11903056
Results sugest that 5T4 is a transient marker of human embryonic stem cell differentiation & that 5T4 phenotype, colony seeding density and culture conditions influence the maintenance of pluripotent hES cells and their differentiation to neural lineages. PMID: 16616918
there is a repertoire of CD8 T cell recognition of 5T4 in normal human donors and some candidate HLA-A*0201 epitopes have been identified PMID: 16646078
the mRNA expression of trophoblast glycoprotein is up-regulated in cells circulating within blood from women with preeclampsia. PMID: 17978129
5T4-based cancer vaccine: cytotoxic T lymphocyte epitopes identified in Trovax-vaccinated colorectal cancer patients PMID: 18567615
colorectal cancer patients who had preexisting cell proliferative responses to 5T4 were longer-term survivors PMID: 18833005

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Database Links

HGNC: 12004

OMIM: 190920

KEGG: hsa:7162

STRING: 9606.ENSP00000358765

UniGene: Hs.71947

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TPBG/5T4: the Oncofetal Protein, an Appealing Target for ADC or Vaccine Therapeutics in Tumors! Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy TGF-beta signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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