Code | CSB-RA024093MA1HU |
Size | US$210 |
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CUSABIO produced the TPBG recombinant monoclonal antibody through a series of steps. Firstly, B cells were obtained from the spleen of the immunized animal, where the recombinant human TPBG protein served as the immunogen during the immunization process. RNA was then isolated from the B cells and converted into cDNA through reverse transcription. Using the cDNA as a template, the gene encoding the TPBG antibody was extended with a degenerate primer and then inserted into a recombinant vector. The vector was introduced into host cells via transfection to enable antibody expression. The TPBG recombinant monoclonal antibody was harvested from the cell culture supernatant and purified using affinity chromatography. To validate the antibody, it underwent testing for reactivity with human and macaca mulatta TPBG proteins in ELISA, confirming its specificity and functionality.
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