Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CDCP1 at 2 μg/mL can bind Anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), the EC50 is 1.861-2.330 ng/mL.
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Expression Region
30-667aa
Target Protein Sequence
FEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHTTMLSIKPGERILFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQFGPGESCPDGVTYSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVIPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQNPGILRLQFQVLVQHPQNESNKIYVVDLSNERATSLTIEPRPVKQSRKFVPGCFVCLESRTCSTNLTLTSGSKHKISFLCDNLTRLWMNVEKNISCTDHRYCQRKSYSLQVPSDIFHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIEQIQVKQNISVTLRTFAPSFRQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERTGVVCQTGRAFMIIQEQRTRAEEIFSLDEDALPKPRFHHHSFWVNISNCSPASGKQLDLLFWVTLTPRTVDLT
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO produced the recombinant Macaca fascicularis CUB domain containing protein 1 (CDCP1) in the mammalian cell expression system. The gene fragment corresponding to the 30-667aa of the Macaca fascicularis CDCP1 protein is fused with a 10xHis-tag gene at the C-terminus and then cloned into a plasmid vector, followed by transfected into mammalian cells. The transfected mammalian cells are cultured for protein expression. The recombinant Macaca fascicularis CDCP1 protein is harvested from the cell lysate. Its purity is over 95% determined by SDS-PAGE. It contains low endotoxin, less than 1.0 EU/ug measured by the LAL method. Its activity is validated in a functional ELISA. Immobilized Macaca fascicularis CDCP1 at 2 μg/mL can bind anti-CDCP1 recombinant antibody (CSB-RA884474MA1HU), with the EC50 of 1.861-2.330 ng/mL.
CDCP1, a novel human tumor-associated gene reported in 2001, is a transmembrane glycoprotein that exhibits limited expression in normal human tissues but is upregulated in most cancers. It plays a crucial role in the initiation, progression, and recurrence of tumors. Clinical studies have indicated an association between elevated CDCP1 expression and poor prognosis in lung cancer, pancreatic cancer, renal cell carcinoma, ovarian cancer, and hepatocellular carcinoma. The CDCP1-regulated signaling pathways are significant in promoting cancer and mediating resistance to chemotherapy and targeted therapy, making it a potential new target for early cancer treatment. The monkey CDCP1 is highly homologous with human CDCP1, with similar structure and functions. Currently, there are limited studies and no clinical drugs targeting CDCP1. Therefore, the development of Macaca fascicularis CDCP1 protein could aid in both clinical drug research and fundamental research related to CDCP1, facilitating the development of clinical treatments and allowing for animal model testing.
