Recombinant Macaca fascicularis Fibroblast activation protein alpha (FAP), partial (Active)

Fibroblast activation protein alpha plays a central role in tumor stromal remodeling and has emerged as a promising target for cancer immunotherapy and diagnostic imaging. This cynomolgus macaque ortholog, spanning residues 26–760 with an N-terminal 10xHis tag, demonstrates specific antibody binding in functional ELISA with an EC50 of 0.6459–0.8172 ng/mL, confirming that the tag placement preserves epitope accessibility for immunoassay development and inhibitor screening campaigns. The >95% purity determined by SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for kinetic parameter analysis, IC50 determination in drug candidate evaluation, and use as a positive control in enzyme-linked assays where contaminants would confound quantitative measurements. The validated binding activity supports applications in substrate specificity profiling and inhibition mechanism studies, providing researchers with a non-human primate model protein suitable for translational oncology investigations where species-specific differences in FAP structure and function are experimentally relevant.