Recombinant Macaca fascicularis Legumain (LGMN) (Active)

Legumain, a lysosomal cysteine protease that cleaves substrates after asparagine residues, plays critical roles in antigen processing, extracellular matrix remodeling, and tumor progression, making it a target of interest in cancer biology and immunology. This full-length cynomolgus macaque ortholog (aa 18–433) demonstrates robust catalytic function with a specific activity exceeding 500 pmol/min/µg against the fluorogenic substrate Z-AAN-AMC, providing a quantitative benchmark for enzymatic activity assays, kinetic parameter determination (Km, Vmax, kcat/Km), and substrate specificity profiling. The C-terminal His tag preserves access to the active site while enabling straightforward purification, and the >95% purity combined with endotoxin levels below 1.0 EU/µg supports accurate kinetic measurements and inhibitor screening campaigns where contaminants could confound IC50 values. Researchers conducting drug candidate evaluation, inhibition mechanism studies, or using legumain as a positive control in enzyme-linked assays will find this preparation meets the quality thresholds commonly required for reproducible enzymology.