Recombinant Macaca mulatta Semaphorin-4D(SEMA4D),partial (Active)

In Stock
Code CSB-MP3947MOW
Size
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC50 is 1.943-2.724 ng/mL. Biological Activity Assay
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Product Details

Description

SEMA4D Macaca mulatta recombinant protein produced in mammalian cells is a single polypeptide containing the amino acid sequence that encodes Phe24 - Arg734 of the Macaca mulatta SEMA4D and having a molecular weight of approximately 90 kDa on SDS-PAGE. This recombinant SEMA4D protein is expressed a 10xHis-tag at C-Terminus. Its purity is greater than 95% measured by SDS-PAGE. It contains low endotoxin, less than 1.0 EU/ug determined by the LAL method. In the functional ELISA, this SEMA4D protein can bind to the anti-SEMA4D recombinant antibody, with EC50 of 1.943-2.724 ng/mL, confirming it is an active protein.

SEMA4D, also known as CD100, plays an important role in the immune and nervous systems. It is involved in platelet inactivation, angiogenesis stimulation, and bone formation regulation. SEMA4D expressed on osteoclasts is a coupling factor that suppresses osteoblast differentiation. As a result, inhibiting SEMA4D could be beneficial to bone growth. SEMA4D is also highly expressed in a variety of tumor tissues, including squamous cell carcinoma of the head and neck, prostate cancer, and colon cancer. SEMA4D-Plexin B1 signaling is frequently dysregulated in malignancies, implying that SEMA4D plays a role in cancer progression, invasion, tumor angiogenesis, tumor-associated macrophage modulation, and skeletal metastasis.

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC50 is 1.943-2.724 ng/mL.
Target Names SEMA4D
Uniprot No. H9FNS0
Alternative Names (EGK_07847)
Molecular Characterization
Species Macaca mulatta (Rhesus macaque)
Source Mammalian cell
Expression Region 24-734aa
Target Protein Sequence FAPIPRITWEHREVRLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSSLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSIAEEVFSHGKYMQSATVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGRRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALYQTESPSRGLVQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPVPTSTSCEPKIVINTVPQLHSEKTMYLKSSDNR
Mol. Weight 81.9 kDa
Protein Length Partial
Tag Info C-terminal 10xHis-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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