Product Details

Purity

>95% as determined by SDSPAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF?1 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10⁶ IU/mg.

Target Names

Ctf1

Uniprot No.

Q60753

Research Area

Immunology

Alternative Names

Ctf1Cardiotrophin-1; CT-1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-203aa

Complete Sequence

MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA

Mol. Weight

21.5 kDa

Protein Length

Full Length

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Cardiotrophin-1 drives gp130-mediated JAK/STAT signaling with roles in cardiac hypertrophy, neuronal survival, and hematopoietic cell regulation, making precise functional studies dependent on highly potent recombinant material. This tag-free, full-length mouse Ctf1 (aa 1–203) demonstrates an ED50 below 0.5 ng/ml in TF-1 cell proliferation assays, corresponding to a specific activity exceeding 2.0 × 10⁶ IU/mg—potency that supports use in JAK/STAT and MAPK pathway activation studies, immune cell differentiation assays, and dose-response calibration as a reference standard in cytokine detection platforms. Endotoxin levels below 1.0 EU/μg ensure that downstream responses in sensitive cell-based systems, including primary immune cell proliferation and in vivo inflammation models, reflect genuine CT-1 signaling rather than LPS-driven artifacts. Purity exceeding 95% by SDS-PAGE, combined with this endotoxin profile, provides a suitable basis for antibody development workflows including immunization, ELISA coating, and Western blot positive controls.

Target Background

Function

Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.

Gene References into Functions

These results suggest that CT-1 may be a potent candidate for the early prevention and treatment of Alzheimer disease. PMID: 28731433
Identify cardiotrophin-1 as a powerful cytokine promoting mesenchymal stromal cell engraftment and thus improving cell therapy of the infarcted myocardium. PMID: 28754835
CT-1 mRNA levels in adipose tissue showed significant circadian fluctuations in young WT mice. Clock genes displayed a circadian rhythm in adipose tissue of both wild-type (WT) and CT-1-/- mice. However, the pattern was altered in CT-1-/- mice toward a lower percentage of the rhythm or lower amplitude, especially for Bmal1 and Clock. PMID: 28096235
Cardiotrophin-1 modulates the production of adipokines in vitro and in vivo, suggesting that the regulation of the secretory function of adipocytes could be involved in the metabolic actions of this cytokine. PMID: 27608275
Nuclear translocation of CT-1 regulates cardiomyogenesis of ES cells and involves calcium, NO, ROS as well as CT-1 regulated signaling pathways. PMID: 26005169
Data suggest that Ctf1 up-regulates lipolysis in white adipocytes via 1) induction of perilipin, 2) activation of hormone sensitive lipase (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of G0S2). PMID: 25351614
Cardiotrophin-1 (CT-1) is a hepatoprotective cytokine that modulates fat and glucose metabolism. Here we analyzed the changes in hepatic fat stores induced by recombinant CT-1 and its therapeutic potential in non-alcoholic fatty liver disease. PMID: 24362075
The transgenic expression of CTF1 brought about a marked improvement on cognitive functioning in the APPswe/PS1dE9 transgenic mouse model of Alzheimer's disease. PMID: 23541490
CT-1 improves beta cell function and survival, and protects mice against STZ-induced diabetes PMID: 23358882
CT-1 may be a major regulator of arterial stiffness with a major impact on the aging process. PMID: 23172930
Hypoxia increased cardiotrophin-1 levels in cardiac cells through a direct regulation of CT-1 promoter by HIF-1alpha, protecting cells from apoptosis. PMID: 21771897
Data suggest a key role for CT-1 in cardiac remodeling induced by aldosterone independent of changes in blood pressure levels. PMID: 21926338
We conclude that CT-1 is a master regulator of fat and glucose metabolism with potential applications for treatment of obesity and insulin resistance. PMID: 21803294
Treatment of embryoid bodies grown from pluripotent murine embryonic stem (ES) cells with cardiotrophin 1 significantly stimulated cardiomyogenesis and increased nuclear expression of the proliferation marker Ki-67. PMID: 15023522
The embryonic neurons of mice regulate the onset of cortical gliogenesis via cardiotrophin-1. PMID: 16242406
In embryonic deficient mice, preganglionic sympathetic neurons were reduced. PMID: 16467531
Induction of interleukin-6 mRNA after infarct was significantly abrogated in CT-1 null mice compared to wild-type mice PMID: 17150369
CT-1 is an essential endogenous defense of the liver against ischemia-reperfusion injury and is a key mediator of the protective effect induced by ischemic preconditioning. PMID: 17178916
Our data show that CT-1 is a natural defense of the liver against apoptosis. This cytokine may have therapeutic potential. PMID: 17326158
adipose tissue can be recognized as a source of CT-1, which could account for the high circulating levels of CT-1 in patients with metabolic syndrome X PMID: 17940213
aldosterone induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms PMID: 18566129
Cardiotrophin-1 is an osteoclast-derived stimulus of bone formation required for normal bone remodeling PMID: 18665789

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Subcellular Location

Secreted.

Protein Families

IL-6 superfamily

Tissue Specificity

Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen.

Database Links

KEGG: mmu:13019

STRING: 10090.ENSMUSP00000049161

UniGene: Mm.389954

Code	CSB-AP000531MO
Abbreviation	Recombinant Mouse Ctf1 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Mouse Cardiotrophin-1 protein (Ctf1) (Active)

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Target Background

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