Recombinant Mouse Cardiotrophin-1 protein(Ctf1) (Active)

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Code CSB-AP000531MO
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Product Details

Purity >95% as determined by SDS?PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF?1 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x106 IU/mg.
Target Names Ctf1
Uniprot No. Q60753
Research Area Immunology
Alternative Names Ctf1Cardiotrophin-1; CT-1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1-203aa
Complete Sequence MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA
Mol. Weight 21.5 kDa
Protein Length Full Length
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 150mM NaCl
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor.
Gene References into Functions
  1. These results suggest that CT-1 may be a potent candidate for the early prevention and treatment of Alzheimer disease. PMID: 28731433
  2. Identify cardiotrophin-1 as a powerful cytokine promoting mesenchymal stromal cell engraftment and thus improving cell therapy of the infarcted myocardium. PMID: 28754835
  3. CT-1 mRNA levels in adipose tissue showed significant circadian fluctuations in young WT mice. Clock genes displayed a circadian rhythm in adipose tissue of both wild-type (WT) and CT-1-/- mice. However, the pattern was altered in CT-1-/- mice toward a lower percentage of the rhythm or lower amplitude, especially for Bmal1 and Clock. PMID: 28096235
  4. Cardiotrophin-1 modulates the production of adipokines in vitro and in vivo, suggesting that the regulation of the secretory function of adipocytes could be involved in the metabolic actions of this cytokine. PMID: 27608275
  5. Nuclear translocation of CT-1 regulates cardiomyogenesis of ES cells and involves calcium, NO, ROS as well as CT-1 regulated signaling pathways. PMID: 26005169
  6. Data suggest that Ctf1 up-regulates lipolysis in white adipocytes via 1) induction of perilipin, 2) activation of hormone sensitive lipase (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of G0S2). PMID: 25351614
  7. Cardiotrophin-1 (CT-1) is a hepatoprotective cytokine that modulates fat and glucose metabolism. Here we analyzed the changes in hepatic fat stores induced by recombinant CT-1 and its therapeutic potential in non-alcoholic fatty liver disease. PMID: 24362075
  8. The transgenic expression of CTF1 brought about a marked improvement on cognitive functioning in the APPswe/PS1dE9 transgenic mouse model of Alzheimer's disease. PMID: 23541490
  9. CT-1 improves beta cell function and survival, and protects mice against STZ-induced diabetes PMID: 23358882
  10. CT-1 may be a major regulator of arterial stiffness with a major impact on the aging process. PMID: 23172930
  11. Hypoxia increased cardiotrophin-1 levels in cardiac cells through a direct regulation of CT-1 promoter by HIF-1alpha, protecting cells from apoptosis. PMID: 21771897
  12. Data suggest a key role for CT-1 in cardiac remodeling induced by aldosterone independent of changes in blood pressure levels. PMID: 21926338
  13. We conclude that CT-1 is a master regulator of fat and glucose metabolism with potential applications for treatment of obesity and insulin resistance. PMID: 21803294
  14. Treatment of embryoid bodies grown from pluripotent murine embryonic stem (ES) cells with cardiotrophin 1 significantly stimulated cardiomyogenesis and increased nuclear expression of the proliferation marker Ki-67. PMID: 15023522
  15. The embryonic neurons of mice regulate the onset of cortical gliogenesis via cardiotrophin-1. PMID: 16242406
  16. In embryonic deficient mice, preganglionic sympathetic neurons were reduced. PMID: 16467531
  17. Induction of interleukin-6 mRNA after infarct was significantly abrogated in CT-1 null mice compared to wild-type mice PMID: 17150369
  18. CT-1 is an essential endogenous defense of the liver against ischemia-reperfusion injury and is a key mediator of the protective effect induced by ischemic preconditioning. PMID: 17178916
  19. Our data show that CT-1 is a natural defense of the liver against apoptosis. This cytokine may have therapeutic potential. PMID: 17326158
  20. adipose tissue can be recognized as a source of CT-1, which could account for the high circulating levels of CT-1 in patients with metabolic syndrome X PMID: 17940213
  21. aldosterone induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms PMID: 18566129
  22. Cardiotrophin-1 is an osteoclast-derived stimulus of bone formation required for normal bone remodeling PMID: 18665789

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Tissue Specificity Highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels in testis and brain. No expression in spleen.
Database Links

KEGG: mmu:13019

STRING: 10090.ENSMUSP00000049161

UniGene: Mm.389954

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