Purity
Greater than 90% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2 μg/mL can bind Mouse Crtam, the EC50 is 54.36 -61.77 ng/mL.
Alternative Names
Class-I MHC-restricted T-cell-associated molecule;CD355
Species
Mus musculus (Mouse)
Expression Region
17-289aa
Target Protein Sequence
AFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQLLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCSTERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPFQFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQHTGLARRKSG
Tag Info
C-terminal hFc-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
PBS, pH 7.4, 6% trehalose
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant mouse Crtam is synthesized through a mammalian cell expression system. The gene fragment encoding mouse Crtam (17-289aa) is fused with a hFc-tag gene at the C-terminus and integrated into a plasmid vector. The recombinant vector is transfected into mammalian cells. Following transfection, protein expression is initiated through cell culture. The resulting recombinant mouse Crtam protein's purity surpasses 95%, verified using SDS-PAGE, and its endotoxin content measures below 1.0 EU/ug using the LAL method. Functionality is confirmed via binding assays with mouse Cadm1 (CSB-MP004425MO), with EC50 of 54.36 -61.77 ng/mL.
Crtam, an emerging immune cell receptor for NK cells, is crucial for the retention of CD8+ T cells and the differentiation of lymph node resident CTL effector cells. When the receptor Crtam expressed on NK cells binds with the ligand Necl-2 expressed on target cells, it enhances NK cell cytotoxicity. Blocking the Crtam-Necl-2 interaction with antibodies could help control the production of inflammatory cytokines. The Crtam receptor and Necl-2 ligand are highly conserved between mice and humans, with homology of 72% and 98% respectively. Developing active and stable Crtam protein can contribute to further exploration of the mechanism and signaling pathway of Crtam-CADM1 interaction, aiding in the development of therapeutic antibodies.
