Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2 μg/mL can bind Mouse Crtam, the EC50 is 54.36 -61.77 ng/mL.

Target Names

Crtam

Uniprot No.

Q149L7

Alternative Names

Class-I MHC-restricted T-cell-associated molecule;CD355

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

17-289aa

Target Protein Sequence

AFLKMETVTVEEGQTLTLTCVTSQTKNVSLQWLAPSGFTIFLNQHPALKSSKYQLLHHSATQLSISVSNVTLREEGVYTCLHYGSSVKTKQVRVTVLVTPFQPTVEALVLRRQNGEKSVVLKCSTERSKPPPQITWLLGEGLEIYGELNHEFEADGKICNTSSMLIARAYGKNSTVHCIIQHEGLHGRKLVAPFQFEDLVADQETSDQETSDAPEQSSLSSQALQQPTSTVSMMENSSIPETDKEEKEHATQDPGLSTEASAQHTGLARRKSG

Mol. Weight

58.9KDa

Protein Length

Partial

Tag Info

C-terminal hFc1-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant mouse Crtam is synthesized through a mammalian cell expression system. The gene fragment encoding mouse Crtam (17-289aa) is fused with a hFc-tag gene at the C-terminus and integrated into a plasmid vector. The recombinant vector is transfected into mammalian cells. Following transfection, protein expression is initiated through cell culture. The resulting recombinant mouse Crtam protein's purity surpasses 95%, verified using SDS-PAGE, and its endotoxin content measures below 1.0 EU/ug using the LAL method. Functionality is confirmed via binding assays with mouse Cadm1 (CSB-MP004425MO), with EC₅₀ of 54.36 -61.77 ng/mL.

Crtam, an emerging immune cell receptor for NK cells, is crucial for the retention of CD8⁺ T cells and the differentiation of lymph node resident CTL effector cells. When the receptor Crtam expressed on NK cells binds with the ligand Necl-2 expressed on target cells, it enhances NK cell cytotoxicity. Blocking the Crtam-Necl-2 interaction with antibodies could help control the production of inflammatory cytokines. The Crtam receptor and Necl-2 ligand are highly conserved between mice and humans, with homology of 72% and 98% respectively. Developing active and stable Crtam protein can contribute to further exploration of the mechanism and signaling pathway of Crtam-CADM1 interaction, aiding in the development of therapeutic antibodies.

Target Background

Function

Mediates heterophilic cell-cell adhesion which regulates the activation, differentiation and tissue retention of various T-cell subsets. Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. Regulates CD8+ T-cell proliferation in response to T-cell receptor (TCR) activation. Appears to be dispensable for CD8+ T-cell-mediated cytotoxicity. Interaction with SCRIB promotes the late phase of cellular polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG, IL17 and IL22 production. By interacting with CADM1 on CD8+ dendritic cells, regulates the retention of activated CD8+ T-cells within the draining lymph node. Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells. Interaction with CADM1 promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells.

Gene References into Functions

These results reveal that CRTAM is critical to instruct the differentiation of CD4(+)CTL through the induction of Eomes and CTL-related gene. PMID: 26694968
During pathogenic infection, Crtam-Cadm1 interactions regulate the dynamic equilibrium between newly formed CD4(+) T cells and their retention in the gut. PMID: 24687959
TCS upregulated the expression of tumor suppressor in lung cancer 1 (TSLC1) in 3LL tumor cells and the expression of its ligand, class I-restricted T cell-associated molecule (CRTAM), in effector T cells. PMID: 21572449
In reciprocal crosses involving alcohol-preferring and alcohol-avoiding mouse strains, Crtam is identified as a candidate gene for alcohol preference. PMID: 19413645
show that CRTAM has an unexpected constitutive expression in adult thymocytes and, remarkably, it is sustained during all stages of thymocyte development. PMID: 19799932
Necl2/CRTAM molecular pair could regulate a large panel of cell/cell interactions both within and outside of the immune system PMID: 15781451
NK cells and T8 cells recognize Necl-2 through CRTAM, expressed only on activated cells. CRTAM-Necl-2 interactions promote cytotoxicity of NK cells and IFN-gamma secretion of T8 cells as well as NK cell-mediated rejection of tumors expressing Necl-2 PMID: 15811952
CRTAM expression is critical for the accumulation of antigen-specific cytotoxic T lymphocytes and their subsequent proliferation within the draining lymph node. PMID: 19752223

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Nectin family

Tissue Specificity

In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT, NK and CD8+ T-cells (at protein level). Transiently expressed in activated CD8+ T-cells and a subset of activated CD4+ T-cells (at protein level). Exp

Database Links

KEGG: mmu:54698

STRING: 10090.ENSMUSP00000034519

UniGene: Mm.42791

Code	CSB-MP614820MO
Abbreviation	Recombinant Mouse Crtam protein, partial (Active)
MSDS	MSDS Datasheet
Size	$114
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Mouse Cadm1(CSB-MP004425MO) at 2μg/mL can bind Mouse Crtam，the EC₅₀ is 54.36 -61.77 ng/mL. Biological Activity Assay
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Recombinant Mouse Cytotoxic and regulatory T-cell molecule (Crtam), partial (Active)

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