Product Details

Purity

≥98% as determined by SDSPAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3?E1 cells is 5-20 μg/ml.

Target Names

Dhh

Uniprot No.

Q61488

Research Area

Stem Cells?

Alternative Names

DhhDesert hedgehog protein; DHH; HHG-3) [Cleaved into: Desert hedgehog protein N-product; Desert hedgehog protein C-product]

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

24-198aa

Complete Sequence

IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASG PAEGRVTRGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG

Mol. Weight

20.0 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered PBS, pH 7.4,1 mM DTT, 0.05 % Tween-80

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Desert hedgehog (Dhh) plays a critical role in male gonad differentiation and peripheral nerve sheath development, making it a key target in stem cell and developmental biology research. This tag-free recombinant mouse Dhh, covering residues 24–198 of the mature signaling domain, demonstrates confirmed biological activity with an ED50 of 5–20 μg/ml in inducing alkaline phosphatase production in MC3T3-E1 cells—a functional readout directly relevant to osteogenic differentiation studies and hedgehog pathway activation assays. The E. coli expression system yields a non-glycosylated protein that provides a suitable basis for receptor-ligand binding experiments, including SPR and competition ELISAs, where glycan-independent interactions are being characterized. Purity exceeding 98% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for cell-based proliferation assays, stem cell differentiation protocols, and use as a quantitative ELISA standard.

Target Background

Function

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Gene References into Functions

the critical role of Dhh in maintaining blood nerve barrier integrity and demonstrates for the first time that endothelial dysfunction is sufficient to induce neuropathy PMID: 26645982
In organogenesis of the ovary, production of Dhh/Ihh in granulosa cells requires growth differentiation factor 9 from the oocyte. PMID: 25917826
increased expression in cell bodies of cutaneous sensory nerves of dorsal root ganglia as compared with skin epithelia PMID: 25842978
The results demonstrate a pivotal role for dhh in maintof this study aining myelination. Furthermore, dhh signaling reveals a potential target for therapeutic intervention to prevent and treat demyelination of peripheral nerves in compression neuropathies PMID: 25936873
Hedgehog signaling is impaired in aged mice. Hegdehog-dependent regulation of angiogenesis and myogenesis involves distinct mechanisms. PMID: 24135022
Data indicate that the centrosomal protein DZIP1 interacts with GLI3 transcription factor in the cytoplasm and regulates Hedgehog signaling and ciliogenesis. PMID: 23955340
Desert hedgehog promotes ischemia-induced angiogenesis by ensuring peripheral nerve survival. PMID: 23343527
Desert hedgehog-dependent transcription in T cells promotes T helper (Th)2 cell transcriptional programs and differentiation, exacerbating allergic disease. PMID: 23408837
During recovery of hematopoiesis after irradiation, and under conditions of stress-induced erythropoiesis, erythrocyte differentiation was accelerated in both spleen and bone marrow of Dhh-deficient mice compared with wild type. PMID: 22461491
The findings suggest that Sertoli cells coordinate Dhh-dependent spermatogenesis events via Ptc1 and Smo prior to the first meiotic division and in postmeiotic (haploid) cells, particularly during the first half of spermiogenesis. PMID: 19484749
Desert Hedgehog/Patched 1 signaling specifies fetal Leydig cell fate in testis organogenesis PMID: 12050120
transgenic skin developed lesions reminiscent of human basal cell carcinoma PMID: 15371305
Prominent role of hedgehog signaling in the communication between granulosa cells and developing theca cells. PMID: 15878962
A single identified glial signal, desert hedgehog, plays a critical role in controlling the integrity of peripheral nervous tissue, in line with its critical role in nerve sheath development. PMID: 16763045
results demonstrate a functional desert hedgehog(HH) pathway in the follicle and identify granulosa cells as at least one of the potential targets of HH signaling PMID: 17392501
combinatorial expression of the paracrine factor Dhh and nuclear transcription factor Sf1 is required for Leydig cell development PMID: 17495005
Inactivation of Nf1 in the glial lineage in vitro at embryonic day 12.5 + 1, but not earlier (neural crest) or later (mature Schwann cell), results in colony-forming cells capable of multilineage differentiation. PMID: 18242511

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Subcellular Location

[Desert hedgehog protein N-product]: Cell membrane; Lipid-anchor; Extracellular side.; [Desert hedgehog protein C-product]: Secreted, extracellular space.

Protein Families

Hedgehog family

Tissue Specificity

Expressed in adult testes. Not expressed in limb buds.

Database Links

KEGG: mmu:13363

STRING: 10090.ENSMUSP00000023737

UniGene: Mm.324688

Code	CSB-AP000541MO
Abbreviation	Recombinant Mouse Dhh protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse Desert hedgehog protein (Dhh), partial (Active)

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Target Background

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