Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active)

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Code CSB-MP020990MO
Size $228
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC50 is 4.878-7.446 ng/ml. Biological Activity Assay
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Product Details

Greater than 95% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC50 is 4.878-7.446 ng/ml.
Target Names
Uniprot No.
Research Area
Alternative Names
(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)
Molecular Characterization
Mus musculus (Mouse)
Mammalian cell
Expression Region
Target Protein Sequence
Mol. Weight
108.1 kDa
Protein Length
Tag Info
C-terminal mFc-tagged
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

The Sema4d was produced as a fusion protein with a C-terminal mFc-tag in mammalian cells. Its expression region corresponds to the amino acid 24-733 of the mouse Sema4d protein. This recombinant Sema4d protein is an active protein and its activity has been validated through the ELISA. In the functional ELISA, this mouse Sema4d protein can bind to the Pepinemab, with the EC50 of 4.878-7.446 ng/ml. The purity of this Sema4d protein is greater than 95% measured by SDS-PAGE. On the gel, this Sema4d protein migrated to the molecular weight band of about 117 kDa. It contains less endotoxin, <1.0 EU/ug determined by the LAL method. And it is available now.

Sema4d is a transmembrane homodimer involved in the inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. It also possesses immune regulatory activity. Studies have shown that Sema4d is hyper-expressed in some malignancies and facilitates tumor growth and angiogenesis.

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Target Background

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Gene References into Functions
  1. These results reveal a novel signaling network, the Sema4D-RhoA-Akt signal cascade, that coordinates cellular function and morphology and highlights the importance of specific spatiotemporally restricted components of a signaling pathway in the regulation of ameloblast differentiation. PMID: 27218883
  2. our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity PMID: 27614265
  3. SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells. PMID: 28416516
  4. Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice PMID: 26752319
  5. Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia PMID: 26202989
  6. The addition of recombinant Sema4D to Sema4D/ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells. PMID: 25351707
  7. Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during pubertal tissue remodeling. PMID: 24841081
  8. therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. PMID: 25252878
  9. CD100-mediated signals are critical for effective activation of gammadelta IEL to produce growth factors, including KGF-1, that are required for healing of the colon epithelium during colitis. PMID: 23695512
  10. Deletion of plexin-B1, the high affinity receptor for semaphorin 4D, significantly (one-way ANOVA; p < 0.001) reduced thrombus weight (- 40%) in oxidative venous thrombosis. PMID: 23642655
  11. Data indicate neuroimmune semaphorin 4D (Sema4D) as an important regulator of Th2-driven lung pathophysiology and as a potential target for a combinatory disease immunotherapy. PMID: 23911404
  12. Sema 4D/CD100 is not only a ligand for plexin B1 but also a direct signaling receptor in mediating Sema 4D/CD100-plexin B's biological functions. PMID: 23262975
  13. CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted gamma-delta T cell response to keratinocyte damage. PMID: 22902232
  14. Sema4D may act as an intrinsic inhibitory regulator of oligodendrocyte differentiation by promoting apoptosis. PMID: 22198439
  15. in vivo, Sema4D is an indirect regulator of bone resorption, which acts via its effect on reproductive function PMID: 22046317
  16. Data show that osteoclasts express semaphorin 4D (Sema4D), previously shown to be an axon guidance molecule, which potently inhibits bone formation. PMID: 22019888
  17. Studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. PMID: 20919742
  18. Loss of Semaphorin 4D is associated with defect in collagen-induced platelet aggregation and an impaired vascular injury response. PMID: 20855865
  19. Findings suggest that Sema4D is involved in the progression phase of atherosclerosis by accelerating intimal neovascularization, resulting in enhanced macrophage infiltration in atherosclerotic plaques. PMID: 20514420
  20. Sema4D enhances inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in microglia. PMID: 20038643
  21. Sema4D-plexin-B2 interactions regulate mechanisms underlying cell specification, differentiation, and migration during corticogenesis. PMID: 19948886
  22. Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury. PMID: 19472224
  23. CD100, which enhances the maturation of DCs, is essential in the activation and differentiation of Ag-specific T cells. PMID: 12133937
  24. Semaphorin 4D triggers invasive growth, a complex programme that includes cell dissociation, anchorage-independent growth and branching morphogenesis. PMID: 12198496
  25. enhances the in vitro activation and differentiation of T cells and the in vivo generation of antigen-specific T cells PMID: 12374982
  26. Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin PMID: 14534257
  27. Sema4D may function as a neurite-outgrowth stimulating factor in embryonic sensory neurons. PMID: 15330859
  28. Strength of B-cell antigen receptor signals is strictly tuned by the interaction of CD100 with CD72; this interaction is essential for maintaining immunological homeostasis as well as generating a proper immune response. PMID: 16113236
  29. CD100 enhances pathogenetic humoral immune responses and promotes the activation of Antigen-Presenting Cells by up-regulating CD86 expression PMID: 16920982
  30. proteolytic cleavage of Semaphorin 4D may provide a novel molecular mechanism by which membrane type 1-matrix metalloproteinase controls tumor-induced angiogenesis PMID: 17204469
  31. semaphorin 4D has a role in platelet responses to vascular injury PMID: 17244710
  32. In the ovarian follicle, the effect of Plexin-B1 is mediated by sema-4D. PMID: 17376242
  33. Sema4D modulates T cell-APC interactions in allogeneic responses both in vivo and in vitro. PMID: 17950916
  34. that tumor-associated macrophages (TAMs) are the main cells producing Sema4D within the tumor stroma and that their ability to produce Sema4D is critical for tumor angiogenesis and vessel maturation. PMID: 18559453
  35. A functional role for CD100 antigen/Plxnb1 interactions in epithelial branching morphogenesis during organogenesis is reported. PMID: 18799546
  36. loss of sema4D (semaphorin 4D) expression reduces the platelet hyperactivity otherwise found in dyslipidemia, and confers protection against the development of atherosclerosis PMID: 19390055
  37. These results suggest that SHP2-mediated tyrosine dephosphorylation is an important step in Sema4D-induced axon repulsion. PMID: 19433062
  38. these findings suggest a novel role of Sema4D/CD100 in the regulation of microglia activation providing a valuable neuroprotective tool PMID: 19467284
  39. Mouse behavioral analyses revealed enhanced motor activity in Sema4D-deficient mice, suggesting the crucial involvement of Sema4D in the neurodevelopmental processes of the central structures mediating motor behavior in mice. PMID: 19534337

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Semaphorin family
Tissue Specificity
Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. Expressed in neurons and glia in the developing hippocampus.
Database Links
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