Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC₅₀ is 4.878-7.446 ng/ml.

Target Names

Sema4d

Uniprot No.

O09126

Research Area

Immunology

Alternative Names

(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)

Molecular Characterization

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

24-733aa

Target Protein Sequence

FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKMVINTVPQLHSEKTVYLKSSDNR

Mol. Weight

108.1 kDa

Protein Length

Partial

Tag Info

C-terminal mFc-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Semaphorin-4D plays a pivotal role in immune cell regulation, influencing T cell activation, B cell responses, and dendritic cell migration through interactions with its receptors Plexin-B1 and CD72. This recombinant mouse Sema4D (aa 24–733) is produced in mammalian cells to preserve native-like post-translational modifications and carries a C-terminal mFc tag that facilitates detection and purification in binding assays. Functional ELISA validation demonstrates robust interaction with immobilized Pepinemab, yielding an EC50 of 4.878–7.446 ng/ml, which supports use in antibody characterization studies, receptor-ligand binding assays, and as a reliable positive control in immunological screening platforms. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for functional cell-based assays investigating Sema4D-mediated immune signaling and for in vitro studies requiring minimal innate immune interference.

Target Background

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.

Gene References into Functions

These results reveal a novel signaling network, the Sema4D-RhoA-Akt signal cascade, that coordinates cellular function and morphology and highlights the importance of specific spatiotemporally restricted components of a signaling pathway in the regulation of ameloblast differentiation. PMID: 27218883
our findings revealed for the first time that Sema4D positively regulated both the adaptive and innate immune responses in contact hypersensitivity PMID: 27614265
SEMA4D-/- neutrophils show impaired NET formation when in contact with endothelial cells. PMID: 28416516
Absence of Sema4D improves oligodendrocyte recovery after cerebral ischemia/reperfusion injury in mice PMID: 26752319
Sema4D-deficiency affected ischemia-induced microglial activation in morphology, iNOS expression, and proliferation, and this deficiency most likely resulted in moderation of cytotoxicity and locomotor behavioral abnormality after cerebral ischemia PMID: 26202989
The addition of recombinant Sema4D to Sema4D/ vaginal epithelial cells in culture significantly enhanced apoptosis of the vaginal epithelial cells. PMID: 25351707
Sema4D may be an essential apoptosis-inducing ligand that acts downstream of estrogen action in vaginal epithelium during pubertal tissue remodeling. PMID: 24841081
therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. PMID: 25252878
CD100-mediated signals are critical for effective activation of gammadelta IEL to produce growth factors, including KGF-1, that are required for healing of the colon epithelium during colitis. PMID: 23695512
Deletion of plexin-B1, the high affinity receptor for semaphorin 4D, significantly (one-way ANOVA; p < 0.001) reduced thrombus weight (- 40%) in oxidative venous thrombosis. PMID: 23642655
Data indicate neuroimmune semaphorin 4D (Sema4D) as an important regulator of Th2-driven lung pathophysiology and as a potential target for a combinatory disease immunotherapy. PMID: 23911404
Sema 4D/CD100 is not only a ligand for plexin B1 but also a direct signaling receptor in mediating Sema 4D/CD100-plexin B's biological functions. PMID: 23262975
CD100 deficiency in vivo resulted in delayed repair of cutaneous wounds due to a disrupted gamma-delta T cell response to keratinocyte damage. PMID: 22902232
Sema4D may act as an intrinsic inhibitory regulator of oligodendrocyte differentiation by promoting apoptosis. PMID: 22198439
in vivo, Sema4D is an indirect regulator of bone resorption, which acts via its effect on reproductive function PMID: 22046317
Data show that osteoclasts express semaphorin 4D (Sema4D), previously shown to be an axon guidance molecule, which potently inhibits bone formation. PMID: 22019888
Studies identify Plxdc1, Sema4D, and neuropilin-1 as novel LRP1-regulated cell-signaling proteins. PMID: 20919742
Loss of Semaphorin 4D is associated with defect in collagen-induced platelet aggregation and an impaired vascular injury response. PMID: 20855865
Findings suggest that Sema4D is involved in the progression phase of atherosclerosis by accelerating intimal neovascularization, resulting in enhanced macrophage infiltration in atherosclerotic plaques. PMID: 20514420
Sema4D enhances inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in microglia. PMID: 20038643
Sema4D-plexin-B2 interactions regulate mechanisms underlying cell specification, differentiation, and migration during corticogenesis. PMID: 19948886
Sema4D is involved in oligodendrogenesis during development and during recovery from ischemic injury. PMID: 19472224
CD100, which enhances the maturation of DCs, is essential in the activation and differentiation of Ag-specific T cells. PMID: 12133937
Semaphorin 4D triggers invasive growth, a complex programme that includes cell dissociation, anchorage-independent growth and branching morphogenesis. PMID: 12198496
enhances the in vitro activation and differentiation of T cells and the in vivo generation of antigen-specific T cells PMID: 12374982
Sema4D is a novel inhibitory factor for axonal regeneration expressed in myelin PMID: 14534257
Sema4D may function as a neurite-outgrowth stimulating factor in embryonic sensory neurons. PMID: 15330859
Strength of B-cell antigen receptor signals is strictly tuned by the interaction of CD100 with CD72; this interaction is essential for maintaining immunological homeostasis as well as generating a proper immune response. PMID: 16113236
CD100 enhances pathogenetic humoral immune responses and promotes the activation of Antigen-Presenting Cells by up-regulating CD86 expression PMID: 16920982
proteolytic cleavage of Semaphorin 4D may provide a novel molecular mechanism by which membrane type 1-matrix metalloproteinase controls tumor-induced angiogenesis PMID: 17204469
semaphorin 4D has a role in platelet responses to vascular injury PMID: 17244710
In the ovarian follicle, the effect of Plexin-B1 is mediated by sema-4D. PMID: 17376242
Sema4D modulates T cell-APC interactions in allogeneic responses both in vivo and in vitro. PMID: 17950916
that tumor-associated macrophages (TAMs) are the main cells producing Sema4D within the tumor stroma and that their ability to produce Sema4D is critical for tumor angiogenesis and vessel maturation. PMID: 18559453
A functional role for CD100 antigen/Plxnb1 interactions in epithelial branching morphogenesis during organogenesis is reported. PMID: 18799546
loss of sema4D (semaphorin 4D) expression reduces the platelet hyperactivity otherwise found in dyslipidemia, and confers protection against the development of atherosclerosis PMID: 19390055
These results suggest that SHP2-mediated tyrosine dephosphorylation is an important step in Sema4D-induced axon repulsion. PMID: 19433062
these findings suggest a novel role of Sema4D/CD100 in the regulation of microglia activation providing a valuable neuroprotective tool PMID: 19467284
Mouse behavioral analyses revealed enhanced motor activity in Sema4D-deficient mice, suggesting the crucial involvement of Sema4D in the neurodevelopmental processes of the central structures mediating motor behavior in mice. PMID: 19534337

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Semaphorin family

Tissue Specificity

Strongly expressed in lymphoid tissues, especially in the thymus, as well as in the nervous tissues. Expressed in neurons and glia in the developing hippocampus.

Database Links

KEGG: mmu:20354

STRING: 10090.ENSMUSP00000021900

UniGene: Mm.33903

Code	CSB-MP020990MO
Abbreviation	Recombinant Mouse Sema4d protein, partial (Active)
MSDS	MSDS Datasheet
Size	$116
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC₅₀ is 4.878-7.446 ng/ml. Biological Activity Assay
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Recombinant Mouse Semaphorin-4D (Sema4d), partial (Active)

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Target Background

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