Greater than 95% as determined by SDS-PAGE.
Less than 1.0 EU/ug as determined by LAL method.
Measured by its binding ability in a functional ELISA. Immobilized Pepinemab at 2 μg/ml can bind Mouse Sema4d, the EC50 is 4.878-7.446 ng/ml.
(M-Sema G)(Semaphorin-C-like 2)(Semaphorin-J)(Sema J)(CD100)
Mus musculus (Mouse)
Target Protein Sequence
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The Sema4d was produced as a fusion protein with a C-terminal mFc-tag in mammalian cells. Its expression region corresponds to the amino acid 24-733 of the mouse Sema4d protein. This recombinant Sema4d protein is an active protein and its activity has been validated through the ELISA. In the functional ELISA, this mouse Sema4d protein can bind to the Pepinemab, with the EC50 of 4.878-7.446 ng/ml. The purity of this Sema4d protein is greater than 95% measured by SDS-PAGE. On the gel, this Sema4d protein migrated to the molecular weight band of about 117 kDa. It contains less endotoxin, <1.0 EU/ug determined by the LAL method. And it is available now.
Sema4d is a transmembrane homodimer involved in the inactivation of platelets, stimulation of angiogenesis, and regulation of bone formation. It also possesses immune regulatory activity. Studies have shown that Sema4d is hyper-expressed in some malignancies and facilitates tumor growth and angiogenesis.