Purity

≥97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of >1.0x10⁶ IU/mg.

Target Names

FLT3LG

Uniprot No.

H9Z6V7

Research Area

Immunology

Species

Macaca mulatta (Rhesus macaque)

Source

E.coli

Expression Region

27-185aa

Complete Sequence

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP

Mol. Weight

18.0 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FLT3 ligand drives the expansion and differentiation of hematopoietic progenitors and dendritic cell precursors, making it an essential tool for immunology research in nonhuman primate models. This tag-free recombinant rhesus macaque FLT3LG (aa 27–185) demonstrates exceptional signaling potency, with an ED50 below 1.0 ng/ml in a human AML5 cell proliferation assay corresponding to a specific activity exceeding 1.0 × 10⁶ IU/mg—supporting use in dendritic cell differentiation protocols, hematopoietic progenitor expansion assays, and JAK/STAT or MAPK signaling pathway studies. Endotoxin levels below 1.0 EU/μg ensure that immune cell responses in functional assays or in vivo primate inflammation models remain attributable to FLT3LG activity rather than LPS-driven artifacts. Purity exceeding 97% by SDS-PAGE, combined with this endotoxin profile, satisfies the criteria typical for reference standards in cytokine detection assays and as a positive control in antibody validation workflows.

Code	CSB-AP003231MOW
Abbreviation	Recombinant Rhesus macaque FLT3LG protein, partial (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Rhesus Macaque Fms-related tyrosine kinase 3 ligand protein (FLT3LG), partial (Active)

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