FLT3LG, or FMS-associated tyrosine kinase 3 ligand, alias FLT3 ligand.FLT3LG is a protein that in humans is encoded by the FLT3LG gene. Its mechanism of action is to promote early hematopoietic cell proliferation by activating its receptor FLT3, which has a good synergy with various other colony-stimulating factors and interleukins.FLT3LG initiates growth and differentiation by binding to the FLT3 receptor and triggering homodimerization and autophosphorylation of the receptor, which activates the downstream signaling pathways such as PI3K/AKT and Ras/MAPK, initiating a growth and differentiation The biological significance of FLT3LG lies mainly in its regulation of the proliferation and differentiation of hematopoietic stem and progenitor cells (HSPC).
Mutations in the FLT3LG gene can lead to severe immunodeficiencies manifested by myelodysplasia, a significant reduction in HSPC, and a lack of monocytes and dendritic cells. In addition, FLT3LG is essential for the differentiation of monocytes and dendritic cells, which play a key role in the body's immune defense.The discovery of FLT3LG not only extends our understanding of the molecular mechanisms controlling the development of the hematopoietic spectrum, but also underscores the important role of FLT3LG in the immune defense, particularly in the immune response against a variety of pathogens including HPV in the immune response against various pathogens, including HPV.
Validated Data
FLT3LG Antibody (CSB-PA008734LA01HU)
Validated Data
Immunofluorescence staining of MCF-7 cells with CSB-PA008734LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of CSB-PA008734LA01HU diluted at 1:800 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
The following FLT3LG reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
FLT3LG Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA008734LA01HU | FLT3LG Antibody | Human | ELISA, IHC, IF |
CSB-PA008734LB01HU | FLT3LG Antibody, HRP conjugated | Human | ELISA |
CSB-PA008734LC01HU | FLT3LG Antibody, FITC conjugated | Human | |
CSB-PA008734LD01HU | FLT3LG Antibody, Biotin conjugated | Human | ELISA |
CSB-PA778110 | FLT3LG Antibody | Human | ELISA,WB |
CSB-PA002551 | FLT3LG Antibody | Human | WB, IHC, ELISA |
CSB-PA006021 | FLT3LG Antibody | Human | WB, ELISA |
FLT3LG Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP008734HU1 CSB-BP008734HU1 CSB-MP008734HU1 CSB-EP008734HU1-B |
Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP008734HU1 | Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG), partial | E.coli |
CSB-AP002201HU | Recombinant Human Fms-related tyrosine kinase 3 ligand protein (FLT3LG), partial (Active) | E.Coli |
CSB-CF008734HU | Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG) | in vitro E.coli expression system |
CSB-AP005901HU | Recombinant Human Fms-related Tyrosine Kinase 3 Ligand (FLT3LG), partial (Active) (GMP) | E.Coli |
CSB-MP008734HU-WD | Recombinant Human Fms-related tyrosine kinase 3 ligand (FLT3LG),Partial (Active) | Mammalian cell |
FLT3LG Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF008734MO | Recombinant Mouse Fms-related tyrosine kinase 3 ligand (Flt3lg) | in vitro E.coli expression system |
CSB-YP008734MO1 CSB-EP008734MO1 CSB-BP008734MO1 CSB-MP008734MO1 CSB-EP008734MO1-B |
Recombinant Mouse Fms-related tyrosine kinase 3 ligand (Flt3lg), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FLT3LG ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04554h | Human FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit | serum, plasma, tissue homogenates | 1.95 pg/mL |
FLT3LG ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04555m | Mouse FMS-like tyrosine kinase 3 ligand,Flt-3L ELISA Kit | serum, plasma, tissue homogenates | 3.9 pg/mL |