β-hydroxybutyryl-HIST1H1D (K75) Antibody

Datasheet
Code CSB-PA010379OA75bhbHU
Size US$319
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate (all treated with 30mM sodium butyrate for 4h)
    All lanes: HIST1H1D antibody at 1.3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • Immunocytochemistry analysis of CSB-PA010379OA75bhbHU diluted at 1:10 and staining in Hela cells (treated with 50mM sodium 3-hydroxybutyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIST1H1D Polyclonal antibody
Uniprot No. P16402
Target Names HIST1H1D
Alternative Names Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of β-hydroxybutyryl-Lys (75) derived from Human Histone H1.3
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, ICC
Recommended Dilution
Application Recommended Dilution
WB 1:100-1:1000
ICC 1:10-1:100
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Subcellular Location Nucleus, Chromosome
Protein Families Histone H1/H5 family
Database Links

HGNC: 4717

OMIM: 142210

KEGG: hsa:3007

STRING: 9606.ENSP00000244534

UniGene: Hs.136857

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