ABCB1 Antibody

Code CSB-PA11737A0Rb
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA11737A0Rb at dilution of 1:50

  • Immunofluorescent analysis of HepG2 cells using CSB-PA11737A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA11737A0Rb at dilution of 1:100

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Product Details

Description
ABCB1 polyclonal antibody CSB-PA11737A0Rb was produced in the rabbit immunized by using the Recombinant Human Multidrug resistance protein 1 protein (236-297AA) as the immunogen. The target protein ABCB1, a member of the superfamily of ATP-binding cassette (ABC) transporters, is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells. Diseases associated with ABCB1 include Colchicine Resistance and Inflammatory Bowel Disease 13.
This Rabbit anti-Homo sapiens (Human) ABCB1 Polyclonal antibody was tested in the ELISA, IHC and IF applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It only reacts with the ABCB1 proteins of human-origin and may be used to detect the endogenous levels of ABCB1 protein.
Full Product Name Rabbit anti-Homo sapiens (Human) ABCB1 Polyclonal antibody
Uniprot No. P08183
Target Names ABCB1
Alternative Names ABC20 antibody; ABCB1 antibody; ATP binding cassette, sub family B (MDR/TAP), member 1 antibody; ATP-binding cassette sub-family B member 1 antibody; CD243 antibody; CLCS antibody; Colchicin sensitivity antibody; Doxorubicin resistance antibody; GP170 antibody; MDR1 antibody; MDR1_HUMAN antibody; Multidrug resistance 1 antibody; Multidrug resistance protein 1 antibody; P glycoprotein 1 antibody; P gp antibody; P-glycoprotein 1 antibody; PGY1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Multidrug resistance protein 1 protein (236-297AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential.. K Qiu,Cells,2020

Applications: Western Blot
Sample type: Muscle Myo-lineage cells
Review: In order to verify the reliability of proteomics data, 7 DEPs were randomly selected for Western blot analysis. As shownin FigureS1, there lativea bundance sof selected proteins between Myo-L and Myo-Y determined by Western blot were highly consistent with the data of TMT analysis.
PMID: 32331484

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Target Data

Function Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Gene References into Functions
  1. Conformational dynamics of P-glycoprotein in lipid nanodiscs and detergent micelles reveal complex motions on a wide time scale. PMID: 29511086
  2. Study demonstrated that TWIST protein expression was elevated in liver cancer tissue specimens and was positively correlated with MDR1 expression. Knockdown of TWIST increased the sensitivity of RHepG2 cells to antineoplastic agents through a reduction in MDR1 expression and drug efflux ability. PMID: 30066890
  3. the over-expression of SLC7A11, or supplementation with sufficiently cystine, or treatment with N-acetylcysteine significantly decreased P-gp expression and activity. It was suggested that ROS and SLC7A11/cystine were the two relevant factors responsible for the expression and function of P-gp, and that SLC7A11 might be a potential target modulating ADR resistance. PMID: 28630426
  4. Data provide a mechanistic explanation for the differential effects of ABCB1 haplotypes on its promoter activity and underscore the importance of evaluating genetic variants in the context of haplotypes rather than individual SNPs when investigating their effects on gene/protein expression and disease risk. PMID: 30277801
  5. CYP3A5, ABCB1 and two POR genotypes were assessed by real-time PCR. PMID: 28094348
  6. ABCB1 C3435T polymorphism can affect the elimination of some antipsychotic/antidepressant drugs. PMID: 29723928
  7. High BCL11A and MDR1 expression was associated with a poor response to chemotherapy. PMID: 29469608
  8. The methylation/expression ratios of ABCB1 were unaffected by increasing BMI values. PMID: 27897267
  9. Neither the ABCB1 C3435T nor the SLCO1B1 T521C polymorphism affected edoxaban PK. PMID: 27897269
  10. Our results show that ABCB1 C3435T polymorphism may modulate serum THC levels in chronic heavy cannabis users. The exact mechanisms and roles that this may play in cannabis dependence genesis and evolution remain to be elucidated. These results should be controlled in a replication study using a larger population. PMID: 28917442
  11. MDR1 is not expressed on erythrocyte membrane. PMID: 29098941
  12. The genetic polymorphisms of the multi-drug resistance-1 (MDR-1) and human cytochrome P450 3A (CYP3A4 and CYP3A5) genes were analyzed and compared between steroid sensitive, steroid resistant and control groups. PMID: 30143489
  13. This meta-analysis suggests that the MDR1 C > T polymorphism was not associated with the risk of MM. To confirm these findings, further comprehensive and well-designed studies are needed. PMID: 29495954
  14. High MDR1 expression is associated with chemoresistance in ovarian cancer. PMID: 29956807
  15. 13-cis-retinoic acid, retinol and retinyl-acetate inhibited the Pgp and ABCG2 mediated substrate transport as well as the substrate stimulated ATPase activity of these transporters. PMID: 28145501
  16. Haplotype analysis of ABCB1 conducted in patients with bullous pemphigoid demonstrated that the 1236T-2677G-3435T haplotype may protect against development of disease. PMID: 29948283
  17. High ABCB1 expression is associated with Bendamustine-resistance in Mantle Cell Lymphoma. PMID: 29695404
  18. Data show that multidrug resistance gene 1 (MDR1) expression was associated with worsen survival of esophageal squamous cell carcinoma (ESCC) patients with cisplatin-based chemotherapy. PMID: 29107111
  19. ABCB1 variation affected myelosuppression, progression-free survival and overall survival in ovarian cancer patients who were treated with paclitaxel and carboplatin PMID: 29504705
  20. Our data showed that the expression of the MDR1 gene was significantly higher in malignant tissue than in the normal tissues of patients with STS. In addition, high MDR1 expression was significantly associated with local advances, as well as poor response to treatment. PMID: 29689707
  21. These results indicated that MG132 reversed the MDR of hypopharyngeal carcinoma by downregulating Pgp/Pgp, and the underlying mechanism may be associated with the activation the of the JNK signaling pathway PMID: 29901180
  22. P-glycoprotein on peripheral blood leukocytes of systemic lupus erythematosus (SLE) patients with lupus arthritis may have a role in clinical response to methotrexate PMID: 28616661
  23. This study elaborated a novel UCA1/miR-129/ABCB1 regulatory axis underlying paclitaxel resistance of ovarian cancer cells. PMID: 29777711
  24. ABCB1 3435C>T and ATIC 347C>G SNPs were associated with abnormal hepatic enzyme elevation by methotrexate treatment in patients with rheumatoid arthritis. PMID: 29252093
  25. The role of multidrug resistance 1 gene (MDR1 or ABCB1) polymorphism G2677T was studied in relation to paroxetine therapeutic efficacy and its side effects, as well as its association with selected demographic and clinical characteristics of patients with depressive disorder. PMID: 29754150
  26. alterations in latrophilin expression occur in AML cells expressing P-gp PMID: 29938681
  27. This study demonstrated that the The G2677T T and C3435T T alleles as well as the TT, CTT and TTT haplotypes seemed to be significantly associated with drug-resistance epilepsy in Tunisian epileptic population. PMID: 29198163
  28. Pharmacokinetics of aripiprazole is affected by sex and ABCB1 gene phenotype PMID: 29325225
  29. There were no significant differences in the bosutinib C0 between genotypes for ABCB1, ABCG2, and CYP3A4 polymorphisms. PMID: 29736778
  30. Studies show a connection between UDP-glucose ceramide glucosyltransferase (UGCG) and multidrug resistance protein 1 (MDR1) overexpression and multidrug resistance development [Review]. PMID: 29409484
  31. Due to the downregulation of MDR1 and MRP1, the intracellular accumulation of ADM was increased in the transfected cells compared with that in the parental K562/ADM cells..Our research revealed that protein expression of the ERK signaling pathway was inhibited by downregulating TRIB2, indicating that the ERK pathway was involved in cell drug resistance and proliferation. PMID: 29436678
  32. Asiatic Acid strongly inhibited P-GP expression by blocking MDR1 gene transcription and increased the intracellular accumulation of the P-GP substrate Rhodamine 123 in MDR1-overexpressing cisplatin (DDP)-resistant lung cancer cells, A549/DDP. PMID: 29768255
  33. Ceritinib enhanced the efficacy of substrate chemotherapeutic agent in ABCB1-overexpressing K562/adr leukemia cells both in vitro and in vivo models, but neither in sensitive parental K562 leukemia cells nor in ABCC1-overexpressing HL-60/adr leukemia cells. PMID: 29742496
  34. Polymorphism of ABCB1 rs1045642-T variant allele is associated with colorectal cancer. PMID: 29282011
  35. There is a certain correlation between the APC gene and ovarian tumors, and the APC gene mediates the apoptosis of tumor cells through the MDR-1/CLCX-1 signaling pathway. PMID: 29921377
  36. ABCB1/Pgp expression, activity, and single nucleotide polymorphisms (SNPs) in chronic myeloid leukemia. [review] PMID: 29316665
  37. High MDR1 expression is associated with primary myelodysplastic syndrome. PMID: 27605311
  38. analysis of the structure of zosuquidar and UIC2-bound human-mouse chimeric ABCB1 PMID: 29440498
  39. ABCB1 gene polymorphisms are not associated with response to chemotherapy in breast cancer. PMID: 29113667
  40. Patients prescribed with short-term low-dose atorvastatin and carrying ABCB1 (rs1128503) or ABCG2 (rs2231142) SNPs did not show differences in LDL-C response (P>.05). PMID: 28833323
  41. Genotypes of UGT1A and ABCB1 were significantly associated with changes in moxifloxacin pharmacokinetic parameters. PMID: 29210323
  42. G2677T/A polymorphism of MDR1 may play a significant role in the development of drug-resistant epilepsy in the Polish patients PMID: 28608314
  43. No association was found between the ABCB1 SNPs and Crohn's disease in the two populations PMID: 28759738
  44. icaritin (ICT) decreased the mRNA and protein levels of multidrug resistance protein 1 (MDR1) in MG-63 doxorubicin-resistant (MG-63/DOX) cells. PMID: 29425587
  45. We demonstrate for the first time that the effect of ABCB1 diplotype on tacrolimus disposition is dependent on both CYP3A5 and CYP3A4 genotype. PMID: 27378609
  46. these results address key concerns in the field, including that of cross-resistance between taxanes and highlighting a mechanism of cabazitaxel resistance involving ABCB1 PMID: 28698198
  47. CYP3A and ABCB1 SNPs were detected in 521 recipients. PMID: 28952408
  48. MDR-1 C3435T gene polymorphism was associated with right ventricular dysfunction in patients with chronic obstructive pulmonary disease. PMID: 29791609
  49. data suggested that hypomethylation agent decitabine restores drug sensitivity in the P-gp-induced MDR phenotype by depressing of P-gp activity as drug pump partly through MAPK signaling pathway. PMID: 28405849
  50. None of the genotypes in ABCB1 1236 C>T, 2677 G>T/A, 3435 C>T, and 4036 A>G correlated with plasma dolutegravir concentration..The speculated peak level of plasma dolutegravir concentration was significantly higher in ABCG2 genetic variant holders, probably, at least in part, because of low expression levels of efflux transporters in the intestines associated with these genetic variants. PMID: 28858994

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Involvement in disease Inflammatory bowel disease 13 (IBD13)
Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
Tissue Specificity Expressed in liver, kidney, small intestine and brain.
Database Links

HGNC: 40

OMIM: 171050

KEGG: hsa:5243

STRING: 9606.ENSP00000265724

UniGene: Hs.489033

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