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ABCB9 Antibody

Datasheet
Code CSB-PA937099
Size US$299
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA937099(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA937099(ABCB9 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Hela cells, Primary antibody: CSB-PA937099(ABCB9 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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Product Details

Uniprot No. Q9NP78
Target Names ABCB9
Alternative Names ABCB9 antibody; KIAA1520ATP-binding cassette sub-family B member 9 antibody; ATP-binding cassette transporter 9 antibody; ABC transporter 9 protein antibody; hABCB9 antibody; TAP-like protein antibody; TAPL antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Synthetic peptide of Human ABCB9
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method Antigen affinity purification
Concentration It differs from different batches. Please contact us to confirm it.
Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Form Liquid
Tested Applications ELISA,WB,IHC
Recommended Dilution
Application Recommended Dilution
ELISA 1:1000-1:2000
WB 1:200-1:1000
IHC 1:15-1:50
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.
Gene References into Functions
  1. The structure of the unique domain of TAPL, is reported. PMID: 24013930
  2. LAMP proteins retain TAPL on the limiting membrane of endosomes and prevent its sorting to intraluminal vesicles. PMID: 22641697
  3. These results suggest that TAPL may be localized to the microdomains (lipid rafts) of lysosomal membranes enriched in cholesterol. PMID: 21212514
  4. By dissecting ABCB9, distinct functions were assigned to the core complex and the extra N-terminal transmembrane domain. PMID: 20377823
  5. is part of the peptide-loading complex in the classic route of antigen processing via major histocompatibility complex class I molecules PMID: 15863492
  6. These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast. PMID: 16554024
  7. the TAPL-specific translocation of peptides into isolated lysosomes strictly requires ATP hydrolysis PMID: 17977821
  8. The ATP-binding of TAPL required Mg(2+), and was observed at neutral pH. Chemical cross-linking experiments suggested that TAPL forms a homodimer in the membrane and under the solubilized conditions. PMID: 18175933
  9. analysis of lipid activation of the lysosomal transport complex ABCB9 PMID: 18434309
  10. These results suggest that the sorting signal for lysosomes is present within the amino-terminal transmembrane domain (Met(1)-Arg(141)) of the TAPL molecule. PMID: 18952056

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Subcellular Location Lysosome membrane, Multi-pass membrane protein
Protein Families ABC transporter superfamily, ABCB family, MHC peptide exporter (TC 3.A.1.209) subfamily
Tissue Specificity Highly expressed in testis, and at moderate levels in brain, spinal cord, and thyroid. Not expressed in monocytes but strongly expressed during differentiation of monocytes to dendritic cells and macrophages.
Database Links

HGNC: 50

OMIM: 605453

KEGG: hsa:23457

STRING: 9606.ENSP00000280560

UniGene: Hs.511951
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