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Product Name:

ACTL6A Antibody

Code : CSB-PA001236ESR2HU
Size : US$167Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot NO. : O96019
Storage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image :
  • All lanes: ACTL6A antibody at 4 ug/ml
    Lane 1: mouse thymus tissue
    Lane 2: mouse spleen tissue
    Lane 3: mouse heart tissue
    Lane 4: Jurkats whole cell lysate
    Lane 5:K562 whole cell lysate
    Lane 6:Hale whole cell lysate
    Lane 7:A549 whole cell lysate
    Lane 8: MCF-7 whole cell lysate
    Secondary
    Goat polyclonal to rabbit at 1/10000 dilution
    Predicted band size: 48,44 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry of paraffin-embedded human ovary using CSB-PA001236ESR2HU at dilution 1:100

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001236ESR2HU at dilution 1:100

  • Chromatin Immunoprecipitation MCF-7(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-ACTL6A or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers(CSB-PP001236HU) against the TFF1 promoter.

Immunogen : Recombinant human Actin-like protein 6A protein (1-280AA)
Raised in : Rabbit
Species Reactivity : Human,Mouse
Tested Applications : ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
Relevance : Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Form : Liquid
Conjugate : Non-conjugated
Storage Buffer : PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification method : Antigen Affinity Purified
Isotype : IgG
Clonality : Polyclonal
Alias : 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, BAF53A,INO80 complex subunit K, ACTL6A, BAF53, BAF53A, INO80K
Species : Human
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