Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ACTL6A Polyclonal antibody

Uniprot No.

O96019

Target Names

ACTL6A

Alternative Names

53 kDa BRG1 associated factor A antibody; 53 kDa BRG1-associated factor A antibody; ACL6A_HUMAN antibody; Actin like 6A antibody; Actin like protein 6A antibody; Actin related protein 4 antibody; Actin related protein antibody; Actin related protein Baf53a antibody; Actin-like protein 6A antibody; Actin-related protein Baf53a antibody; ACTL 6 antibody; ACTL 6A antibody; ACTL6 antibody; ACTL6A antibody; Arp4 antibody; ARPN BETA antibody; ArpNbeta antibody; BAF 53A antibody; BAF complex 53 kDa subunit antibody; BAF53 antibody; BAF53A antibody; BRG1 associated factor antibody; BRG1-associated factor 53A antibody; hArpN beta antibody; INO80 complex subunit K antibody; INO80K antibody; MGC5382 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse,Rat

Immunogen

Synthesized peptide derived from internal of Human ACL6A.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,WB,IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:3000
IF	1:100-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Gene References into Functions

hese results suggest that BAF53a may be a novel prognostic factor for glioma patients, and that BAF53 may facilitate glioma progression by promoting proliferation, invasion, and associate with EMT. Therefore, BAF53a could be a potential promising biomarker and a target for the treatment of glioma. PMID: 29039584
ACTL6A gene mutation analysis should be considered in patients with intellectual disability, learning disabilities, or developmental language disorder. PMID: 28649782
ACTL6A and p63 collaborate as oncogenic drivers in head and neck squamous cell carcinoma (HNSCC). PMID: 28041841
Study shows high expression level of ACTL6A in osteosarcoma tissues and associates with poor survival providing evidence that ACTL6A promotes osteosarcoma cell metastasis through epithelial-mesenchymal transition. PMID: 28260090
ACTL6A protein expression predicts poor prognosis of hepatocellular carcinoma and metastasis. PMID: 26698646
BAF53 is prerequisite for maintaining the structural integrity of chromosomal subdomains. PMID: 26242195
These results indicate that failure to downregulate the BAF53a subunit may contribute to the pathogenesis of rhabdomyosarcoma, and suggest that BAF53a may represent a novel therapeutic target for this tumor. PMID: 23728344
Data show that ACTL6a prevents SWI/SNF complex binding to promoters of KLF4 and other differentiation genes and that SWI/SNF catalytic subunits are required for full induction of KLF4 targets. PMID: 23395444
The beta-actin-Arp4 complex formation might be a crucial feature in some chromatin-modifying enzyme complexes, such as the Brg1 complex. PMID: 22573825
These results suggest that activated expression of the E6 and E7 genes of integrated HPV is dependent on BAF53-dependent higher-order chromatin structure or nuclear motor activity. PMID: 21821000
study finds Baf53 and Baf170 are highly regulated in HIV-1-infected cells;innate function of Baf53-containing complexes appears to be transcriptionally suppressive PMID: 21699904
data show that BAF53 shuttles between the cytoplasm and nucleus in an energy-dependent manner, and suggest that BAF53 can play a role distinct from its previously recognized function in transcriptional regulation PMID: 14503849

Hide All

Involvement in disease

ACTL6A mutations have been found in patients with intellectual disability of variable severity, developmental delay, dysmorphic features and digit abnormalities. Additional features may include genitourinary and cardiac defects. The disease phenotype resembles Coffin-Siris syndrome and brachymorphism-onychodysplasia-dysphalangism syndrome.

Subcellular Location

Nucleus.

Protein Families

Actin family

Database Links

HGNC: 24124

OMIM: 604958

KEGG: hsa:86

STRING: 9606.ENSP00000397552

UniGene: Hs.435326

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-PA294225
Size	US$297
	Order now
Image	Western blot analysis of extracts from Jurkat cells and COLO205 cells, using ACL6A antibody. Immunofluorescence analysis of HUVEC cells, using ACL6A antibody.
Have Questions?	Leave a Message or Start an on-line Chat

ACTL6A Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background