ADAM29 Antibody

Datasheet

Code	CSB-PA891722LA01HU
Size	US$166
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Image	IHC image of CSB-PA891722LA01HU diluted at 1:500 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA891722LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ADAM29 Polyclonal antibody

Uniprot No.

Q9UKF5

Target Names

ADAM29

Alternative Names

A disintegrin and metalloproteinase domain 29 antibody; ADA29_HUMAN antibody; ADAM 29 antibody; ADAM metallopeptidase domain 29 antibody; ADAM29 antibody; Cancer/testis antigen 73 antibody; CT73 antibody; Disintegrin and metalloproteinase domain-containing protein 29 antibody; Metallaproteinase disintegrin (ADAM29) antibody; svph1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Disintegrin and metalloproteinase domain-containing protein 29 protein (701-820AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ADAM29 Antibody (Product code: CSB-PA891722LA01HU) is Non-conjugated. For ADAM29 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891722LB01HU	ADAM29 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891722LC01HU	ADAM29 Antibody, FITC conjugated
Biotin	CSB-PA891722LD01HU	ADAM29 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

May be involved in spermatogenesis and fertilization. Seems to be a non catalytic metalloprotease-like protein.

Gene References into Functions

Increased transcript expression of ADAM29 was observed in breast cancer tissues compared to normal ones. The expression of ADAM29 and its mutations in different domains significantly influenced proliferation, migration and invasion of breast cancer cells in vitro. PMID: 26977022
PCMC expression of ADAM29, FLRT2, and SLC18A3 could be assessed as part of a routine screen to help identify individuals at risk of severe Obstructive sleep apnea in Asian populations PMID: 24732660
mutated ADAM27 and ADAM7 genes could play a role in melanoma progression. PMID: 21618342
quantification of LPL and ADAM29 gene expression is a strong prognostic indicator in CLL, providing better prognostic assessment than ZAP-70 in advanced stages of the disease PMID: 15802535
Results support the use of LPL and ADAM29 gene expression associated to IGHV mutational status for predicting the clinical outcome of patients treated by oral fludarabine + cyclophosphamide and could be considered for treatment strategies. PMID: 19340428

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Involvement in disease

Has been found to be frequently mutated in melanoma. ADAM7 mutations may play a role in melanoma progression and metastasis.

Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed specifically in testes.

Database Links

HGNC: 207

OMIM: 604778

KEGG: hsa:11086

STRING: 9606.ENSP00000352177

UniGene: Hs.126838

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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