AFP Antibody

Datasheet
Code CSB-PA09987A0Rb
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P02771
Image
  • IHC image of CSB-PA09987A0Rb diluted at 1:750 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunogen Recombinant Human Alpha-fetoprotein protein (19-609AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC; Recommended dilution: IHC:1:500-1:1000
Relevance Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Alpha-fetoprotein (Alpha-1-fetoprotein) (Alpha-fetoglobulin), AFP, HPAFP
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cardiovascular
Target Names AFP
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Involvement in disease Alpha-fetoprotein deficiency (AFPD); Alpha-fetoprotein, hereditary persistence (HPAFP)
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Database Links

HGNC: 317

OMIM: 104150

KEGG: hsa:174

STRING: 9606.ENSP00000379138

UniGene: Hs.518808

Pathway Hippo signaling pathway

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