Human Alpha-fetoprotein,AFP ELISA Kit

Instructions
Code CSB-E04770h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name alpha-fetoprotein
Alternative Names FETA, HPAFP, OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
Abbreviation AFP
Uniprot No. P02771
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 1.563 ng/mL-100 ng/mL
Sensitivity 1.332 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Involvement in disease Alpha-fetoprotein deficiency (AFPD); Alpha-fetoprotein, hereditary persistence (HPAFP)
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma. Synthesized by the fetal liver and yolk sac.
Database Links

HGNC: 317

OMIM: 104150

KEGG: hsa:174

STRING: 9606.ENSP00000379138

UniGene: Hs.518808

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.