AGO3 Antibody

Code CSB-PA875700LA01HU
Size US$166
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Image
  • IHC image of CSB-PA875700LA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) AGO3 Polyclonal antibody
Uniprot No.
Target Names
AGO3
Alternative Names
Protein argonaute-3 (Argonaute3) (hAgo3) (Argonaute RISC catalytic component 3) (Eukaryotic translation initiation factor 2C 3) (eIF-2C 3) (eIF2C 3), AGO3, EIF2C3
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein argonaute-3 protein (251-400aa)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The AGO3 Antibody (Product code: CSB-PA875700LA01HU) is Non-conjugated. For AGO3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA875700LB01HU AGO3 Antibody, HRP conjugated ELISA
FITC CSB-PA875700LC01HU AGO3 Antibody, FITC conjugated
Biotin CSB-PA875700LD01HU AGO3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:100-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
Gene References into Functions
  1. Data show that recombinant AGO3 loaded with miR-20a cleaves complementary target RNAs. PMID: 29040713
  2. Study uncovers a first example of a vertebrate protein factor, Argonaute-3, specifically affecting the guide-to-passenger-strand ratio of the miRNA let-7a. A multi-layered mechanism for the observed impact of Ago3 on the let-7a-3p passenger strand expression and activity is proposed. PMID: 24100239
  3. The N-terminal domain of Ago3 inhibits cleavage activity. AAs 1-64 are largely unstructured & form a large loop surrounding an area containing two helices & another unstructured loop. PMID: 23665583
  4. The Ago3 PIWI domain is slicing competent but our Ago3 model suggests multiple interactions between residues in the PIWI & N domains that may increase protein rigidity or invoke other properties that contribute to Ago3's observed slicing deficiency. PMID: 23748378
  5. DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. PMID: 23064648
  6. Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression PMID: 22474261
  7. reliable predictions of miRNA affinity to Ago2 AND Ago3 proteins were made. PMID: 21634124
  8. The specificity of RNA interference depends on the concentration of Ago1, Ago3, and Ago4 relative to Ago2. PMID: 18771919
  9. EIF@C3 protein is expressed in both Schwann and neuron-type differentiating cells. PMID: 19393748

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Subcellular Location
Cytoplasm, P-body.
Protein Families
Argonaute family, Ago subfamily
Database Links

HGNC: 18421

OMIM: 607355

KEGG: hsa:192669

STRING: 9606.ENSP00000362287

UniGene: Hs.657659

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